International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 3 2012 07 10 Micropropagation of an Anti diabetic Plant - Stevia rebaudiana Bertoni, (Natural Sweetener) in Hadoti Region of South-East Rajasthan, India 37 42 EN Patel AnuradhaJitendra 1 Department of Zoology, Bhavans College, Andheri, Mumbai University, Mumbai, INDIA Thakor AkankshaPravin 2 Department of Biotechnology, N.B. Mehta Science College, Bordi, Mumbai University, Mumbai, INDIA Avlessi Félicien 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN AlitonouGuy Alain 2 Institut des Biomolécules Max Mousseron, IBMM UMR, CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE DjenontinT. Sébastien 3 Tchobo Fidele 4 Yèhouénou Boniface 5 MenutChantal 6 Sohounhloué Dominique 7 Radha K. 1 Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA Mahima R. 2 Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA RamanathanG. 3 Thangap 4 ian V. 5 Bhatt T.K. 1 Department of Biotechnology, Central University of Rajasthan, Kishangarh, INDIA DjenontinTindo S. 1 1Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey – Calavi (EPAC), Cotonou, BENIN Amusant N. 2 CIRAD, UMR 016 GPEB, Montpellier, FRANCE Dangou J. 3 CIRAD, UMR ECOFOG, Equipe Matériaux et Molécules, Kourou, FRANCE Wotto D.V. 4 CIRAD, UR 40 Tropical Wood Products, Biorefinery team, Montpellier, FRANCE Avlessi F. 5 Dahouénon-Ahoussi E. 6 Lozano P. 7 PiochD. 8 Sohounhloué K.C.D. 9 Majumdar Tania 1 Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata-700032, INDIA ChakrabortyRunu 2 Raychaudhuri Utpal 3 Mehta Jitendra 1 Plant Tissue Culture Lab. and Dept. of Biotech., Vital Biotech Research Inst., University of Kota, Kota-Rajasthan, INDIA Sain Monika 2 SharmaDev Ratan 3 Gehlot Priyanka 4 SharmaPriyanka 5 DhakerJayraj Kiran 6 > Research Paper 2012 6 8 2012 07 10 This review highlights the recent development and achievements made for the micropropagation of Stevia rebaudiana Bertoni (an antidiabatic sweetener herb) in Hadoti region of south-east Rajasthan. Shootlets were regenerated from nodal explants of stem through auxiliary shoot proliferation. The induction of multiple shoots from nodal segments was the highest in MS medium supplemented with 0.5 mg/l BAP+2.0 mg/l Kn. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium with 1.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to misthouse. Copyright@ International Science Community Association