International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 3 2012 07 10 Study on Enzyme-Linked Immunoassay and Polymerase Chain Reaction for the Identification and Quantification of Staphylococcus aureus in Pure Culture and Food Samples 30 36 EN Patel AnuradhaJitendra 1 Department of Zoology, Bhavans College, Andheri, Mumbai University, Mumbai, INDIA Thakor AkankshaPravin 2 Department of Biotechnology, N.B. Mehta Science College, Bordi, Mumbai University, Mumbai, INDIA Avlessi Félicien 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN AlitonouGuy Alain 2 Institut des Biomolécules Max Mousseron, IBMM UMR, CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE DjenontinT. Sébastien 3 Tchobo Fidele 4 Yèhouénou Boniface 5 MenutChantal 6 Sohounhloué Dominique 7 Radha K. 1 Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA Mahima R. 2 Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA RamanathanG. 3 Thangap 4 ian V. 5 Bhatt T.K. 1 Department of Biotechnology, Central University of Rajasthan, Kishangarh, INDIA DjenontinTindo S. 1 1Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey – Calavi (EPAC), Cotonou, BENIN Amusant N. 2 CIRAD, UMR 016 GPEB, Montpellier, FRANCE Dangou J. 3 CIRAD, UMR ECOFOG, Equipe Matériaux et Molécules, Kourou, FRANCE Wotto D.V. 4 CIRAD, UR 40 Tropical Wood Products, Biorefinery team, Montpellier, FRANCE Avlessi F. 5 Dahouénon-Ahoussi E. 6 Lozano P. 7 PiochD. 8 Sohounhloué K.C.D. 9 Majumdar Tania 1 Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata-700032, INDIA ChakrabortyRunu 2 Raychaudhuri Utpal 3 > Research Paper 2012 6 5 2012 07 10 Search for rapid, accurate and sensitive method of detection for pathogen such as Staphylococcus aureus (S.aureus) has become a challenge to food and pharmaceutical industries. Our present work is a study on molecular methods of detection of food borne pathogen, S.aureus NCIM 2602. Studies showed that protein A (SpA) antigen of S.aureus actually contributed to the resistance of the organism to phagocytosis and opsonization. SpA was detected and the interaction between the SpA antigen and mammalian IgG was confirmed by Western blot and Enzyme linked immunosorbent assay (ELISA). Further, the technique was successfully applied to artificially contaminated food samples like milk, meat, and cheese with an inoculation level of 100 CFU/ml of S.aureus. Polymerase chain reaction (PCR) was also applied for the identification of the gene coding for SpA (spa) for the current strain. These techniques would be useful in analyzing safety of food samples especially meat and dairy based products which are prone to contamination. Copyright@ International Science Community Association