International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 3 2012 07 10 Survey on Drug Resistant Pattern of Clinical Isolates and Effect of Plant Extract on the Drug Resistant Pattern 14 19 EN Patel AnuradhaJitendra 1 Department of Zoology, Bhavans College, Andheri, Mumbai University, Mumbai, INDIA Thakor AkankshaPravin 2 Department of Biotechnology, N.B. Mehta Science College, Bordi, Mumbai University, Mumbai, INDIA Avlessi Félicien 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN AlitonouGuy Alain 2 Institut des Biomolécules Max Mousseron, IBMM UMR, CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE DjenontinT. Sébastien 3 Tchobo Fidele 4 Yèhouénou Boniface 5 MenutChantal 6 Sohounhloué Dominique 7 Radha K. 1 Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA Mahima R. 2 Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA RamanathanG. 3 Thangap 4 ian V. 5 > Research Paper 2012 5 11 2012 07 10 Drug resistance is an emerging fact of the human beings leading to an increase in mortality rate. For this instance clinical samples were collected such as urine, pus, sputum, and catheter samples from private clinical laboratories, Madurai. The following organisms were isolated and characterized as E. coli (49/111), Klebsiella pneumonia (28/111), proteus mirabilis (8/111), pseudomonas aeruginosa (18/111) and staphylococcus aureus (8/111). Further these clinical isolates were subjected to SDS treatment or pomegranate extract treatment for plasmid curing and continued with antibiotic susceptibility test. In our present study, ceftriaxone was found to be more active than any other antibiotics. On the contrary, penicillin G was found to be inactive against all the clinical isolates. The plasmid DNA was separated by agarose gel electrophoresis in E. coli and pseudomonas aeruginosa. Thus resistant organisms, which was examined as multidrug resistance was taken into account for molecular characterization of gene responsible. The plasmids were cured in all the isolates by sodium dodeyl sulphate (or) pomegranate extract. There was no correlation between plasmid pattern and their antibiogram. Copyright@ International Science Community Association