International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 3 2012 07 10 Antioxidant Activity and Phenolic Compounds of Vitex Trifolia Var, Simplicifolia Associated with Anticancer 65 68 EN Patel AnuradhaJitendra 1 Department of Zoology, Bhavans College, Andheri, Mumbai University, Mumbai, INDIA Thakor AkankshaPravin 2 Department of Biotechnology, N.B. Mehta Science College, Bordi, Mumbai University, Mumbai, INDIA Avlessi Félicien 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN AlitonouGuy Alain 2 Institut des Biomolécules Max Mousseron, IBMM UMR, CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE DjenontinT. Sébastien 3 Tchobo Fidele 4 Yèhouénou Boniface 5 MenutChantal 6 Sohounhloué Dominique 7 Radha K. 1 Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA Mahima R. 2 Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA RamanathanG. 3 Thangap 4 ian V. 5 Bhatt T.K. 1 Department of Biotechnology, Central University of Rajasthan, Kishangarh, INDIA DjenontinTindo S. 1 1Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey – Calavi (EPAC), Cotonou, BENIN Amusant N. 2 CIRAD, UMR 016 GPEB, Montpellier, FRANCE Dangou J. 3 CIRAD, UMR ECOFOG, Equipe Matériaux et Molécules, Kourou, FRANCE Wotto D.V. 4 CIRAD, UR 40 Tropical Wood Products, Biorefinery team, Montpellier, FRANCE Avlessi F. 5 Dahouénon-Ahoussi E. 6 Lozano P. 7 PiochD. 8 Sohounhloué K.C.D. 9 Majumdar Tania 1 Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata-700032, INDIA ChakrabortyRunu 2 Raychaudhuri Utpal 3 Mehta Jitendra 1 Plant Tissue Culture Lab. and Dept. of Biotech., Vital Biotech Research Inst., University of Kota, Kota-Rajasthan, INDIA Sain Monika 2 SharmaDev Ratan 3 Gehlot Priyanka 4 SharmaPriyanka 5 DhakerJayraj Kiran 6 Sharma Ch 1 Department of Biotechnology, Himachal Pradesh University, Shimla-171005, INDIA erK. 2 KanwarShamsher S. 3 Ben C.P. 1 Department of Botany, Nesamony Memorial Christian College, Marthandam, Kanyakumari – 629165, Tamilnadu, INDIA BeulaA. 2 Department of Biochemistry, Sri Adi Chunchanagiri Womens College, Cumbum-625516, Tamilnadu, INDIA NirmalaDevi S. 3 Somani Vaishali 1 Zoology Department, B.N. Bandodkar College of Science, Chendani, Thane 400 601, Maharashtra, INDIA QuadrosGoldin 2 Zoology Department, Maharishi Dayanand College, Parel, Mumbai 400 012, Maharashtra, INDIA PejaverMadhuri K. 3 Sálim Ali Centre for Ornithology and Natural History, Anaikatti Post, Coimbatore 641108, Tamil Nadu, INDIA Putri L.S.E. 1 Dept. of Bio., Faculty of Sci. and Tech., State Islamic University, Syarif Hidayatullah Jakarta, Ciputat, INDONESIA HidayatA.F. 2 Department of Biology, Jakarta State University, INDONESIA Suk 3 ar P. 4 Aweng E.R. 1 Universiti Malaysia Kelantan, Locked Bag No 100, 17600 Jeli, Kelantan, MALAYSIA Forest Research Institute Malaysia, 52110 Kepong, Selangor, MALAYSIA Nur Hanisah 2 MohdNawi M.A. 3 NurhananMurniY. 4 Shamsul M. 5 > Short Communication 2012 5 31 2012 07 10 Vitex trifolia var.simplicifolia plant was found growing wildly at the beach, especially at Pantai Sri Tujuh, Tumpat, Kelantan, Malaysia. The extracted leaves were commonly used to prepare traditional dessert among Siamese communities in Kelantan called “Khanom Bai Kunthi” for a long time. Water was used as an extraction agent by the peoples in this area. This extracted leaves were used by the Siamese communities in Tumpat area because it has natural color, flavor and fragrance properties. The Siamese communities who consumed “Khnom Bai Kunthi” was found to be healthy life and live longer. This is the starting point of the study. For the purpose of this study, Vitex trifolia var.simplicifolia leaves were extracted by using 80% methanol in the laboratory. The extracted V. trifolia var.simplicifolia was analyzed and found to be high in antioxidant activity of phenolic compound. The result shows that the extracted V. trifolia var. simplicifolia had high amount of total phenolic compound (44.66µg of GAE/mg of fresh weight). The plants secondary metabolites normally play role in cancer treatment. Cytotoxicity activity of the extracted V. trifolia var.simplicifolia was evaluated on MCF-7 (Breast cancer cell line), HT-29 (colorectal cancer cell line), and WRL-68 (normal liver cell line), and the results show that, cytotoxicity activity (IC50 values 78.87µg/ml, 77.50µg/ml, and 78.29µg/ml, respectively). Copyright@ International Science Community Association