International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 2 2012 June 10 Potential Antimicrobial Agent from Cocos nucifera mesocarp extract; Development of a New Generation Antibiotic 48 54 EN Muhammad Jamshed Khan 1 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Asad Abbas 2 University of Salmanca, SPAIN Mazhar Ayaz 3 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Naeem Muhammad 4 Institute of Applied and Pure Biology, Bahauddin Zakariya University, Multan, PAKISTAN Saleem Qadir 5 University of Agriculture, Faisalabad, PAKISTAN Muhammad Saleem Akhter 6 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Zia Waseem 7 Livestock and Dairy department, Government of Punjab, PAKISTAN P. Muthusamy 1 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA S. Murugan 2 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA Smitha Manothi 3 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA Nishi K. 1 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA N.K. Gupta 2 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA S.C. Sharma 3 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA Kumar Praveen G.N. 1 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA Sumangala K. Bhat 2 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA Nacua Sherwin S. 1 Biology Department, Mindanao State University, Marawi City, PHILIPPINES Torres Mark Anthony J. 2 Department of Biological Sciences, MSU-Iligan Institute of Technology, 9200 Iligan City, PHILIPPINES Cesar G. Demayo 3 Department of Biological Sciences, MSU-Iligan Institute of Technology, 9200 Iligan City, PHILIPPINES Chaitra R. Hegde 1 Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA Madhuri M. 2 Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA Nishitha Swaroop T. 3 Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA Arijit Das 4 Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA Sourav Bhattacharya 5 Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA Rohit K.C. 6 Sapthagiri College of Engineering, Bangalore, INDIA Manju Junwal 1 Department of Zoology, Govt. Madhav Science College, Ujjain, MP, INDIA Ismail Bhai 2 Department of Zoology, Govt. Madhav Science College, Ujjain, MP, INDIA Jayshree Jatwa 1 Department of Zoology Govt. Madhav Science College Ujjain-456010 INDIA Ismail Bhai 2 Department of Zoology Govt. Madhav Science College Ujjain-456010 INDIA V. Verma 1 Presently Molecular Sciences and Nanotechnology, College of Eng. and Science, Louisiana Tech University, Ruston 71270 USA A. Bhardwaj 2 Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA S. Rathi 3 1Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA R.B. Raja 4 1Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA > Research Paper 2012 5 23 2012 June 10 Cocos nuciferamesocarp has been used from time immemorial in the treatment of various skin ailments. Cocos nuciferamesocarp extract can also be used as an antimicrobial agent against clinical pathogens. The purpose of this study was to confirm the anti-bacterial effect of cocos nuciferamesocarp powder using escherichia coli and salmonella typhi. For this six different solvent extracts of cocos nuciferamesocarp powder were produced and the anti-bacterial activity was determined using disc diffusion method. The specific therapeutic compounds were isolated using thin layer chromatography (TLC) and qualitative analysis was performed by high performance liquid chromatogaphy (HPLC) and fourier transform infra-red spectroscopy (FTIR). The antimicrobial activity was found to be highest in case of benzene solvent against E.coli. In the case of salmonella typhi the antimicrobial activity was found to be highest with diethyl ether. TLC provided two fractions of the coconut shell extract powderwhichwere eluted further. FTIR graphs provided characteristic peaks which represented the components responsible for antimicrobial activity. HPLC helped to identify the active biocomponents as tocopherol, palmitoleyl alcohol, cycloartanol and -sitosterol. The Cocos nuciferamesocarp powder can be utilized to develop indigenous antibiotics which can replace conventional antibiotics. Copyright@ International Science Community Association