International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 1 2012 05 10 Decolorization of Reactive Black HFGR by Aspergillus sulphureus 55 61 EN Bi Shaziya 1 School of Studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, INDIA Goyal P.K. 2 Mishra P.C. 1 P.G.Department of Environmental Sciences, Sambalpur University, Jyoti Vihar, Orissa, INDIA Dash A.K. 2 Environmental Engineering, Department of Civil Engineering, ITER ,SOA University ,Bhubaneswar, INDIA Khageswar Pradhan 3 NTPC ,Kaniha ,Angul ,Odisha, INDIA Namdhari B.S. 1 Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, INDIA Rohilla S.K. 2 Department of Energy and Environmental Sciences, Chaudhary Devi Lal University, Sirsa, INDIA Salar R.K. 3 Gahlawat S.K. 4 Bansal P. 5 Saran A.K. 6 Goswami A.P. 1 Department of Biology, M.V.M. Science and Home Science College, Rajkot, Gujarat, INDIA Mankodi P.C. 2 Department of Zoology, Faculty of Science, The M.S. University of Baroda, Vadodara, Gujarat, INDIA Bhosale P.R. 1 Department of Environmental Science, Shivaji University, Kolhapur, MS, INDIA Chonde S.G. 2 Department of Microbiology, Rajaram College, Kolhapur, MS, INDIA Nakade. D.B 3 Raut P.D. 4 Bhatt T.K. 1 Department of Biotechnology, Central University of Rajasthan, Kishangarh, INDIA Dangwal L.R. 1 Herbarium and Plant Systematic Lab., Department of Botany, H.N.B. Garhwal University (a Central University), SRT Campus, Badshahithaul, Tehri Garhwal, Uttarakhand, INDIA Singh Tajinder 2 Salar RajKumar 1 Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, INDIA Rohilla SureshKumar 2 Department of Molecular Biology and Biotechnology, CCS HAU, Hisar, INDIA Rohilla JitenderKumar 3 > Research Paper 2012 1 25 2012 05 10 The strain Aspergillus sulphureus was evaluated for its ability to decolorize a textile dye, Reactive Black HFGR. The effect of physiochemical parameters (shaking vs static, pH, dye concentration and different carbon source) on the degradation of textile dye was studied in C-limited Czapek Dox broth. It was found that shaking favored to dye decolorization efficiency of fungal strain showed highest decolorization (93.04±1.86%). Effect of different concentration of Reactive Black HFGR dyes ranging from 50 to 300 mg/L had a significant effect on decolorization (69.94±1.09 - 93.04±1.86%) their maximal limits at 200 mg/L and increase in concentration of dye showed a negative effect on the decolorization percentage of the tested fungal strain. There was significant decolorization by the fungal strain with different pH ranging from 4 to 8. However, optimum pH was found to be pH 5. There was an influence of carbon source on decolorization as the fungus showed considerable variation in decolorization activity in the carbon supplemented medium. The fungal strain showed maximum activity with sucrose (93.04±1.86%) followed by glucose (85.31±0.59%) and fructose (78.71±1.44%). Therefore, Aspergillus sulphureus is an efficient strain for the decolorization of reactive textile dyes, and it might be a practical alternative in the dyeing wastewater treatment. Copyright@ International Science Community Association