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Biodegradation of crude oil using efficient biosurfactant producing microorganisms

Author Affiliations

  • 1School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamilnadu, India
  • 2School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamilnadu, India

Res. J. Recent Sci., Volume 7, Issue (9), Pages 1-6, September,2 (2018)


Pollution of sea by crude oil caused by stranding of tankers is one of the urgent and serious environment issues over the world. Biosurfactant are formed as extracellular compounds or localized on cell surface of microorganisms. Therefore in the present study focus of attention was given with the foresight of using biosurfactant as a promising tool to emulsify the polluted oils prior to biodegradation. When microbes grow in hydrocarbon contaminated site it undergoes may adaptations. It influences the uptake of hydrocarbon as substrate. Crude biosurfactant helps the biodegradation of hydrocarbon using biosurfactnat producing bacteria to gain better access to their hydrophobic substrates since it bring reduction of surface tension of the marine ecosystem around the bacterium. In the present study, crude oil degradation with mineral salt medium (MSM) the Infrared spectrum of Pseudomonas aeruginosa, Pseudomonas stutzeri, Bacillus cereus, Bacillus licheniformis and control which produce biosurfactant was confirmed by FTIR analysis. The result on bacterial cell growth in all the experiments revealed that biosurfactant producing bacterial cells utilized crude oil as the carbon and energy source which was evident from cell growth observed with experimental Set up exhibited by extraction of crude oil revealed 81.4%, 85.6%, 77.2% and 68.3% of crude oil biodegradation by Pseudomonas aeruginosa, Pseudomonas stutzeri, Bacillus cereus, Bacillus licheniformis, standard and control. Synthetic biosurfactant as standard n-hexane revealed 88.6% during biodegradation of crude oil and complete absence of microorganisms served as control Set exhibited 12% respectively.


  1. Cunha C.D., Do Rosario M., Rosado A.S. and Leite S.G.F. (2004)., Serratia sp. SVGG16: a promising biosurfactant producer isolated from tropical soil during growth with ethanol-blended gasoline., Process Biochemistry, 39(12), 2277-2282.
  2. Kosaric N. (1992)., Biosurfactants in industry., Pure and Applied Chemistry, 64(11), 1731-1737.
  3. Atlas R. and Bragg J. (2009)., Bioremediation of marine oil spills: when and when not–the Exxon Valdez experience., Microbial biotechnology, 2(2), 213-221.
  4. Lal B., Reddy M.R.V., Agnihotri A., Kumar A., Sarbhai M.P., Singh N., Khurana S.K., Khazanchi S.K. and Misra T.R. (2009)., A process for enhanced recovery of crude oil from oil wells using novel microbial consortium., World Intellacetual Property Organization, Patent No.WO/2005/005773.
  5. Atlas R.M. (1995)., Petroleum biodegradation and oil spill bioremediation., Marine Pollution Bulletin, 31(4-12), 178-182.
  6. Delille D. (2000)., Response of Antarctic soil bacterial assemblages to contamination by diesel fuel and crude oil., Microbial Ecology, 40(2), 159-168.
  7. Yakimov M.M., Timmis K.N. and Golyshin P.N. (2007)., Obligate oil-degrading marine bacteria., Current opinion in biotechnology, 18(3), 257-266.
  8. Benincasa M. (2007)., Rhamnolipid produced from agroindustrial wastes enhances hydrocarbon biodegradation in contaminated soil., Current microbiology, 54(6), 445-449.
  9. Penet S., Vendeuvre C., Bertoncini F., Marchal R. and Monot F. (2006)., Characterisation of biodegradation capacities of environmental microflorae for diesel oil by comprehensive two-dimensional gas chromatography., Biodegradation, 17(6), 577-585.
  10. Rahman K.S.M., Banat I.M., Thahira J., Thayumanavan T. and Lakshmanaperumalsamy P. (2002)., Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant., Bioresource technology, 81(1), 25-32.
  11. Atlas R.M. and Bartha R. (1972)., Degradation and mineralization of petroleum in sea water: limitation by nitrogen and phosphorous., Biotechnology and Bioengineering, 14(3), 309-318.