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Isolation of indigenous glyphosate degrading microbes from selected agro ecological zones of Malawi

Author Affiliations

  • 1Ministry of Agriculture, Chitedze Agricultural Research Station box 158 Lilongwe, Malawi
  • 2Centre for Biotechnology and Bioinformatics, University of Nairobi P.O BOX 30197-00100, Nairobi, Kenya
  • 3University of Malawi, chancellor college box 208 Zomba, Malawi
  • 4CAVS University of Nairobi P.O BOX 30197-00100, Nairobi, Kenya

Res. J. Recent Sci., Volume 6, Issue (12), Pages 1-8, December,2 (2017)

Abstract

Weed infestation is one of the remarkably causes for the decrease in yield and yield components for all type crops in Malawi. To minimize losses herbicides control like glyphosate is used because it surpasses other methods employed by farmers since it’s supported by the current strategy of green revolution techniques, timely and cheap. The residuals of glyphosate compounds have an impact and effect on immunity, bio argumentation, bio-magnification, and environment. It is therefore important to investigate synchronized strategies that can degrade of glyphosate because no study has been performed showing prospect microbes in bioremediation study in Malawi. Microbes were isolated for their capacity to utilize glyphosate as sole carbon and phosphorous source complimented by presence of laccase gene. Biochemical test and molecular characterization using 16S rDNA and 18S rDNA genes for bacteria and fungus respectively were used in identification and this was supplemented by testing for the presence of Plant Growth Promoting (PGP). Several fungi and bacteria were found to degrade glyphosate; Mucor irregularis, Fusarium oxysporum, Meyerozyma caribbica, Aspergillus parasiticus for fungus and genus Achromobacter and Enterobacter and for bacteria. 90 % of strains had PGP traits besides potential in bioremediation. The study also adds new strains that can be used in degradation of glyphosate.

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