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Stomata length and Density as an Indicator of Ploidy level in Sweet Pepper (Capsicum annuum L.)

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Author Affiliations

  • 1Horticulture Research Division, NARC, Khumaltar, Nepal
  • 2Kangwon National University, Republic of Korea

Res. J. Recent Sci., Volume 5, Issue (ISC-2015), Pages 4-10, -----Select----,2 (2016)

Abstract

Ploidy level determination in plant is normally done by either chromosome number count in the cell or flow cytometry analysis methods which are tedious and complicated. Hence, the suitability of the stomatal length and density as a criterion in the distinction between haploid (2n=12) and diploid (2n=24) plants of Sweet Pepper (Capsicum annuum L.) was tested in anther culture derived plants from seven cultivars; Derby, Special, Bossanova, Boggie and Minipaprika with 170 plants. Plants were grown in screen house and at least ten fully developed leaves from a plant were measured for its length, width and stomata impression were prepared. Stomata number and length were recorded in 1000x microscope. Result showed that mean stomata length and number were 26.4±2.4µm and 7.4±1.80µm in haploid, 35.2±2.5µm and 5.8±0.8µm in diploid that was 33.3 percent (%) and -30% higher in diploid as compared to haploid. Likewise, mean leaf length and width in haploid was 7.2±1.2 cm and 3.98±0.97 cm and in diploid 9.66±1.7 cm and 6.3±1.0cm that was 34.2% and 65.8% respectively higher in diploid as compared to haploid. Hence, haploid and diploid were significantly different in these parameter and measurement of stomata length is a rapid technique for identifying ploidy level in Sweet pepper.

References

  1. Osuji J.O. and Okoli B.E. (1996)., An improvedprocedure for meiotic studies of the Eumusa section ofthe genus Musa L. (Musaceae), , Info Musa, 5,12-14.
  2. Tenkouano A., Crouch J.H., Crouch H.K. and VuylstekeD. (1998)., Ploidy determination in Musagerm plasmusing pollen and chloroplast characteristics, , Amer J. HortSci., 33(5), 889-890
  3. Dalezel J. (1997)., Application of flow cytometry for thestudy of plant genomes., J. of Appl Genet 38(30), 285-302.
  4. Tarn G.Y. and Dunn G.M. (1973)., Relationship of meterto ploidy level in Bromusinermis Leyss., Crop Science,13, 332-334.
  5. Borrino E.M. and Powell W. (1988)., Stomatal guard celllength as an indicator of ploidy in microspore derivedplants of Barley., Genome, 30, 158-160.
  6. Cohen D. and Yao J.L. (1996)., In vitro chromosomedubling of nine Zantedeschia cultivars., Plant Cell TissueOrgan Cult., 47, 43-49.
  7. Thomas T.D., Bhatnagar A.K. and Bhojwani S.S. (2000)., Production of triploid plants of mulberry (Morusalba L.)by endosperm culture., Plant Cell Rpt, 19, 395-399.
  8. Czabajska W. (1963)., Tetraplodalne form yrumiankupospolitego (Matricaria chamomilla L.)orazichprzydatnosc w hodowli nowych odmianuprawowych (Tetraploid forms of chamomile(Matricaria chamomilla L.) and their usefulness forbreeding of new cultivars), Ph.D. dissertation,Wydawnictwo IRiPZ, Pozan.,
  9. Letchamo W., Marquard R. and Friedt W. (1994)., Alternative methods for determination of ploidy level inchamomile (Chamomilla recutita (L.) Rausch.) breeding., J. Herbs Spices Med. Plants, 2, 19.
  10. Dabrowska J. (1996)., The number of chloroplasts instomata guard cells a useful character for separatingpolyploids and diploids, Beitragezur Zuchtungforschung, ,1, 239.
  11. Przywara L., Pandey K.K. and Sanders P.M. (1988)., Length of stomata as an indicator of ploidy level inActinidiadeliciosa., Newland Journal of Botany, 26, 179-182.
  12. Van Duren M., Morpugo R., Dolezel J. and Afza R.(1996)., Induction and verification of autotetraploids indiploid banana (Musaacuminata) by in vitro techniques., Euphytica, 88, 25-34.
  13. Dolezel J. (1998)., Flow cytometry, its application andpotential for plant breeding., In: Lelley, T.(ed) Currenttopics in plant cytogenetics related to plant improvement,Uinversita stsverlag. Vienna, p.80-90.
  14. Munoz M., Riegel R. and Seemann P. (2006)., Use ofimage cytometry for the early screening of inducedautopolyploids, Plant Breeding, , 125, 414-416.
  15. Burbulis N., Kuprience R. and Zilenaite L. (2004)., Embryogenesis, callogenesis and plant regeneration fromanther cultures of spring rape (Brassica rapus L.). ActaUniv., Biol., 676, 153-158.
  16. Lozykowska K.S. (2003)., Determination of the ploidylevel in chamomile (Chamomillarecutita L. Rausch)strains rich in ʆ-bisabolel. J.Appl. Gent, , 44(2), 151-155.
  17. Gresens I. (1996)., Polyploidisierung durchColchicinierung von Saturejahortensis L. and Origanummajorana L. Drogenreport, , 15, 36.
  18. Evans A. (1955)., The production and identification ofpolyploids in red clover, white clover and Lucerne., Newphytologist,54, 149-162.
  19. Speckmann G.T., Post J. and H. Dijkstra (1965)., Thelength of stomata as an indicator for polyploidy in ryegrasses., Euphytica, 14, 225-230.
  20. Geok-Yong T. and Dunn G.M. (1973)., Relationship ofstomatal length and frequency and pollen-grain diameterto ploidy level in Bromusinermis Leyss., Crop Science,13,332-334.
  21. Dumas D.V.R., Chmbonnet D. and Pochard E. (1981)., Culture in vitro D’antheres de piment (Capsicum annuumL.): amelioration des tuaxd’obtention de plantes chezdifferents genotype par des traitments a +350C,Agronomice, , 1, 859-864.