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Stomata length and Density as an Indicator of Ploidy level in Sweet Pepper (Capsicum annuum L.)

Author Affiliations

  • 1Horticulture Research Division, NARC, Khumaltar, Nepal
  • 2Kangwon National University, Republic of Korea

Res. J. Recent Sci., Volume 5, Issue (ISC-2015), Pages 4-10, -----Select----,2 (2016)


Ploidy level determination in plant is normally done by either chromosome number count in the cell or flow cytometry analysis methods which are tedious and complicated. Hence, the suitability of the stomatal length and density as a criterion in the distinction between haploid (2n=12) and diploid (2n=24) plants of Sweet Pepper (Capsicum annuum L.) was tested in anther culture derived plants from seven cultivars; Derby, Special, Bossanova, Boggie and Minipaprika with 170 plants. Plants were grown in screen house and at least ten fully developed leaves from a plant were measured for its length, width and stomata impression were prepared. Stomata number and length were recorded in 1000x microscope. Result showed that mean stomata length and number were 26.4±2.4µm and 7.4±1.80µm in haploid, 35.2±2.5µm and 5.8±0.8µm in diploid that was 33.3 percent (%) and -30% higher in diploid as compared to haploid. Likewise, mean leaf length and width in haploid was 7.2±1.2 cm and 3.98±0.97 cm and in diploid 9.66±1.7 cm and 6.3±1.0cm that was 34.2% and 65.8% respectively higher in diploid as compared to haploid. Hence, haploid and diploid were significantly different in these parameter and measurement of stomata length is a rapid technique for identifying ploidy level in Sweet pepper.


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