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Biochemical Analysis of Lipids and Proteins in three Freshwater Teleosts (Clarias batrachus, Channa punctatus, Anabas testudineus)

Author Affiliations

  • 1P.G. Dept of Zoology, Berhampur University-760007, Odisha, India
  • 2P.G. Dept of Zoology, Berhampur University-760007, Odisha, India
  • 3P.G. Dept of Zoology, Utkal University-751004, Odisha, India

Res. J. Recent Sci., Volume 5, Issue (6), Pages 24-33, June,2 (2016)


The study deals with the separation and estimation of protein and lipids in different tissues like liver, pancreases, gills and muscles of Clarias batrachus, Channa punctatus and Anabas testudineus. The protein and lipid contents were measured in the three species irrespective of their sex and sizes. The annual mean of protein content in liver was found to be 13.92 2.326, 11.87 0.811, 10.25 0.52 and in pancreases 11.410.794 ,10.63 0.494, 8.85 0.67 gms per 100 grams of tissues in Clarias batrachus, Channa punctatus and Anabas testudineus respectively. It was also observed that the proximate composition of fish depends mostly on season, size, age, sex, reproductive cycle as well as breeding season. Similarly, the mean protein in muscle and gills was estimated to be 19.49 0.732, 17.50 0.865, 18.22 0.931 and 6.079 2.472, 14.779 1.084, 7.80 0.596 respectively among the above species. Lipids content was more in liver and muscles. The mean lipids of liver and muscle were 1.08 0.460 , 1.10 0.118, 0.88 0.462 and 1.46 0.262,1.44 0.39 , 1.19 0.27 respectively among the three species. Comparatively less quantity of lipids are estimated in the pancreases and gills. The mean lipids content of pancreases and gills was found to be 0.789 0.371 ,1.23 0.484 , 1.06 0.298 and 0.81 0.109 , 0.67 0.192 , 1.04 0.328 respectively. All biochemical parameters differ significantly (P<0.001) among the three species under investigation.


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