International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Labscale Production and Purification of Cellulase Enzyme from Aspergillusniger

Author Affiliations

  • 1Department of Microbiology, Moolji Jaitha College, North Maharashtra University, Jalgaon, 425001, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 124-126, September,2 (2015)

Abstract

The aim of this paper is to determine the production of cellulase enzyme from fungal strain Aspergillusniger on labscale. Cellulase enzyme and various cellulosic product obtained from microorganisms have shown to have immense industrial potential by using to chemical cellulase as being traditionally used in industries such as a, food industry, textile industry, paper and pulp industry, biofuel production, brewing and agricultural industry etc. cellulase being broadly used for industrial purpose and the enzyme cellulase being an extremely complex system, research oriented work for its production has importance periodically. The availability of substrate for the production of this enzyme was abundant. The most abundant carbohydrate polymer in biosphere is cellulose, having an annual production that estimated to be over 7.5 1010tones. Cellulase is the inducible enzyme produced by various microorganisms like bacteria, fungi while there growth on carbon containing sources that is cellulose used as a substrate. As compare to bacteria; fungi was most suitable microbes for the enzyme production. .Filamentous fungi that is slender thread like structure of fungi is mostly preferred for cellulase enzyme production and it has good capacity to produced extracellular enzyme. The analysis of production of cellulase enzyme was done by Di-nitrosalycyclic acid (DNS) method .The present study aimed to screen fungi Aspergillus niger for the production of cellulase enzyme and further enzyme purification.

References

  1. Sri Lakshmi S. and Narasimha G., Production of cellulases by fungal cultures isolated from forest litter soil, Ann For. Res., 55(1), 85-92, (2012)
  2. Alzahrani Z., Salting in , Salting out and Dialysis of Protein., BCH, 233, (2010)
  3. Devi C.M, Kumar S.M., Production, optimization and partial purification of cellulose by A. niger fermented with paper and timber sawmill industrial wastes. J. Microbiol, Biotech. Res., 2(1), 120-128, (2012)
  4. Adney B. and Baker J., Measurement of Cellulase Activities, NREL/TP, 5(1),426-428, (1996)
  5. Ghosh T.K., Measurement of cellulase Activities. Pure and Appl Chem, 59(2), 257-268, (1987)
  6. Elad Y., Biological control of foliar pathogens by means of Trichodermaharzianumand potential modes of action. Crop Prot., 19, 709-714,(2000)
  7. Beguin P. and Aubert J.P., The biological degradation of cellulose. FEMS Microbiol. Rev., 13, 25-28, (1994)
  8. Bhat M.K., Cellulases and related enzymes in biotechnology, Biotechnol. Adv. 18, 355-383, (2000)
  9. Miller G.L., Use of Dinitrosalycylic Acid Reagent for Determination of Reducing sugar, Anal. Chem., 3(1), 426-428 (1959)
  10. Sohail M., Ahmad A., Khan S.A., Comparative study of production of cellulase from three strain of A. niger. Dipartment of microbiology, Karachi, 46(5), 1911-1914, 2014)
  11. Upadhyay A. et.al., Biophysical chemistry, Principles and Techniques, 301-343, (1993)