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Isolation of Rhizobacteria from Paddy Field and Their Traits for Plant Growth Promotion

Author Affiliations

  • 1Department of Microbiology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat, Gujarat. INDIA

Res. J. Recent Sci., Volume 4, Issue (IVC-2015), Pages 34-41, (2015)


Rice (Oryza sativa L.) is among the three most important cereal crop of the world and its production exceeds from that of wheat. Rhizobacteria has profound effect on plant growth. Present study was carried out to know the effect of rhizobacteria on growth of paddy and specifically the availability of nutrients mainly Nitrogen, Phosphate and Potassium to the Rice. Total 63 such bacteria were isolated on various media from the rhizospheric soil, Ectorhizospheric and Endorhizospheric region along with Bulk soil of paddy field. The isolates were screened in vitro for significant traits viz., qualitative detection of their nitrogen fixing ability, phosphate solubilization and potassium solubilization, Catalase production and enzyme production viz, Protease, Amylase, Lipase Cellulase etc. The plant growth promoting hormones like Indole Acetic Acid (IAA) and Gibberellic Acid (GA) have also been tested quantitatively. Qualitatively nitrogen was fixed (42), phosphate solubilized (57) and potassium solubilized (21). Rhizobacteria also control growth of plant pathogens by producing enzymes like Protease (30), Amylase (30), Lipase (29) and Cellulase (29). Significant amount of Indole Acetic Acid ranging from 5.79 µg/ml to 43.03 µg/ml and Gibberellic Acid ranging from 118.41 µg/ml to 198.18 µg/ml were produced by the isolates. Results indicates that these rhizobacteria may be exploited further for their ability to increase availability of major nutrient and phytohormone supply to the rice and controlling plant pathogens so as to increase productivity of paddy field..


  1. Juanda J.I.H., Screening of soil bacteria for Plant Growth Promoting Activities in Vitro, J. Agri. Sci., 4, 27-31 (2005)
  2. Gandhi A., Muralidharan G., Sudhakar E., Isolation and identification of elite phosphate solubilizing bacteria from soil under paddy cultivation., International Letters of Natural Sciences., 11(1), 62-68 (2014)
  3. Shrivastava1 A.K., Khilendra Dewangan and Shrivastava D.K., Plant growth promoting rhizobacterial strains from rice rhizospheric soil., Int.J.Curr.Microbiol.App.Sci., 3(4), 774-779 (2014)
  4. Bhattacharyya P. and Jha D., Plant growth promoting rhozobacteria (PGPR): emergence in agriculture, World J Microb Biot., 28(4), 1327-1350 (2011)
  5. Khan M.S., Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities, 163, 173-181 (2006)
  6. Tan, K.Z., O. Radziah, M.S. Halimi, A.R. Khairuddin, S.H. Habib and Z.H. Shamsuddin., Isolation and characterization of rhizobia and plant growth-promoting rhizobacteria and their effects on growth of rice seedlings., American Journal of Agricultural and Biological Sciences 9(3), 342-360, (2014)
  7. Laskar Folguni, Sharma G.D. and Deb B., Characterization of Plant Growth Promoting Traits of Diazotrophic Bacteria and their Inoculating Effects on Growth and Yield of Rice Crops., GRA, 2(4), 2-5 (2013)
  8. Yasuyuki Hashidoko; Tada, Motohiko; Osaki, Mitsuru; Tahara, Satoshi., Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants., Biosci., Biotechnol., Biochem., 66(10), 2259-2263, (2002)
  9. Pikovsakaya RE., Mobilization of phosphorus in soil in connection with vital activity of some microbial species, Microbiologia.,17, 362-370 (1948)
  10. Aleksandrov V.G., Blagodyr R.N. and Iiiev I.P., Liberation of phosphoric acid from apatite by silicate bacteria. Mikrobiology Zh (Kiev), 29, 111-114 (1967)
  11. Gothwal R.K., Nigam V.K., Mohan M. K., Sasma D. and Ghosh P., Screening of nitrogen fixers from rhizospheric bacterial isolates associated with important desert plants, Appl. Ecol and Environ. Res. 6(2), 101-109 (2008)
  12. Smibert RM and Krieg NR., Phenotypic characterization. In: Methods for General and Molecular Biology,. Eds. GerhardtP., Murray R.G.E., Wood W.A, Krieg N.R. American Society for Microbiology, Washington, D.C., 607–654 (1994)
  13. Alariya Shyam Sunder, Sethi Sonia, Gupta Sakhsam and Gupta B. Lal., Amylase activity of a starch degrading bacteria isolated from soil, Arch. of Appl. Sci. Res., 5(1), 15-24 (2013)
  14. Sarkar Sarada B., Sreekanth, Kant Shreya, Banerjee Rintu and Bhattacharyya B.C., Production and optimization of microbial lipase, Bioprocess Eng., 19, 29-32 (1998)
  15. Aneja K.R. Experiments in Microbiology, Plant Pathology, Tissue Culture and Mushroom Production Technology. New Age International Limited, 2nd Edition, (2001)
  16. Gordon S.A and Paleg L.G., Quantitative measurement of Indole acetic acid, Physiol. Plantarum., 10, 347-348 (1957)
  17. Borrow A., Brain P.W., Chester U.E., Curtis P.J., Hemming H.G., Jeffereys E.C., Lloyd R.B., Nixon I.S., Norris G.L.F. and Radley N., Gibberellic acid a metabolic product of fungus Gibberella fujikuroi some observation on its production and isolation, Jour. Sci. Food. Agric., 6, 340-348 (1955)
  18. Reyes V.A. and Valduz Z., Phosphate solubilizing micro-organisms isolated from the rhizospheric and bulk soils of colonizer plants at an abandoned rock phosphate mine, Plant Soil, 287, 69-75 (2006)
  19. Kloepper J.W., Plant-growth-promoting rhizobacteria as biological control agents, in: Soil Microbial Ecology: applications in agricultural and environmental management, F.B. Jr., Metting, ed., Marcel Dekker inc., N.Y., 255-273 (1992)
  20. Ajay Kumar, Amit Kumar, Shikha Devi, Sandip Patil, Chandani Payal and Sushila Negi., Isolation, screening and characterization of bacteria from Rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study., RRST, 4(1), 01-05 (2012)
  21. Kai M., Haustein M.F., Petri A., Scholz B., Piechulla B., Bacterial volatiles and their action potential., Appl Microbiol Biotechnol, 81, 1001–1012 (2009)
  22. Spaepen S. and Vanderleyden J., Auxin and plant-microbe interactions, Cold Spring Harb. Perspect. Biol., 3(4), 1-14, (2011)
  23. Tsavkelova E.A., Klimova S.Y., Cherdyntseva T.A. and Netrusov A.I., Microbial producers of plant growth stimulators and their practical use: a review, Appl. Biochem. Microbiol., 42(2), 117–126, (2006)34-41 (2015)
  24. Crozier A, Kamiya Y, Bishop G and Yokota T., Biosynthesis of hormones and elicitor molecules. In: Biochemistry and molecular biology of plants. American Society of Plant Physiology, Rockville, 850–929 (2000)
  25. Davies P.J., The plant hormones: Their nature, occurrence and functions. In: Plant hormones: Physiology, Biochemistry and Molecular biology, P.J. Davis, ed, Kluwer, Dordrecht, 1–12 (1995)
  26. King R.W. and Evans L.T., Gibberellins and flowering of grasses and cereals: prising open the lid of the “Florigen” black box. Annu Rev Plant Physiol Plant Mol Biol., 54, 307–328 (2003)
  27. Pharis R.P and King R.W., Gibberellins and reproductive development in seed plants, Annu Rev Plant Physiol., 36, 517–568 (1985)
  28. Sponsel V.M., Gibberellins. In: Henry HL, Norman AW (eds) Encyclopedia of hormones, 2, Academic, 29–40 (2003)
  29. Raval A.A. and Desai P.B., Rhizobacteria from Rhizosphere of Sunflower (Helianthus annuus L.) and their effect on Plant Growth, Res. J. Recent Sci., 1(6), 58-61 (2012)