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Isolation of Rhizobacteria from Paddy Field and Their Traits for Plant Growth Promotion

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Author Affiliations

  • 1Department of Microbiology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat, Gujarat. INDIA

Res. J. Recent Sci., Volume 4, Issue (IVC-2015), Pages 34-41, (2015)

Abstract

Rice (Oryza sativa L.) is among the three most important cereal crop of the world and its production exceeds from that of wheat. Rhizobacteria has profound effect on plant growth. Present study was carried out to know the effect of rhizobacteria on growth of paddy and specifically the availability of nutrients mainly Nitrogen, Phosphate and Potassium to the Rice. Total 63 such bacteria were isolated on various media from the rhizospheric soil, Ectorhizospheric and Endorhizospheric region along with Bulk soil of paddy field. The isolates were screened in vitro for significant traits viz., qualitative detection of their nitrogen fixing ability, phosphate solubilization and potassium solubilization, Catalase production and enzyme production viz, Protease, Amylase, Lipase Cellulase etc. The plant growth promoting hormones like Indole Acetic Acid (IAA) and Gibberellic Acid (GA) have also been tested quantitatively. Qualitatively nitrogen was fixed (42), phosphate solubilized (57) and potassium solubilized (21). Rhizobacteria also control growth of plant pathogens by producing enzymes like Protease (30), Amylase (30), Lipase (29) and Cellulase (29). Significant amount of Indole Acetic Acid ranging from 5.79 µg/ml to 43.03 µg/ml and Gibberellic Acid ranging from 118.41 µg/ml to 198.18 µg/ml were produced by the isolates. Results indicates that these rhizobacteria may be exploited further for their ability to increase availability of major nutrient and phytohormone supply to the rice and controlling plant pathogens so as to increase productivity of paddy field..

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