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A Cytogenetic Study on Some Perennial Trigonella (Fenugreek) in Iran

Author Affiliations

  • 1 Fars Research Center for Agriculture and Natural Resources, Shiraz, IRAN

Res. J. Recent Sci., Volume 4, Issue (5), Pages 10-13, May,2 (2015)


Trigonella (Fenugreek) is a plant in the family of Papilionaceae. Its origin is Iran and west Asia and then, it has been taken to other parts of the world. At present, it is grown in most European, Asian and African countries. The research cytogenetically studied some perennial trigonella (fenugreek). It was assessed as a case study in Iran. After germination of seeds, the root apical meristem was used for karyotypic studies. Then, it was stabilized, hydrolyzed and stained. At the next stage, the microscopic sample was provided and the morphology of chromosomes was studied. The results showed that all assessed genotypes were diploid with 2n=2x=16 and the chromosomal basis of x=8. At the next stage, genomes including length of long and short arms of chromosome, the ratio of long arm to short arm and the ratio of short arm to long arm were analyzed. Then, the results were studied through Levan’s (et al.) table, the types of chromosomes were identified and Karyotypic symmetry of species was assessed according to the Stebbins’ method. According to the Stebbins’ bilateral table, genotypes of 460 (T.elliptica, Zanjan, Iran), 770 (T.elliptica, Avaj-Qazvin, Iran) and 5310 (T.elliptica, Shurjestan-Fars, Iran) were classified as group 1A (IA), the genotype of 162 (T.elliptica, Hossein Abad-Fars, Iran) was classified as group 2A, and finally, the genotype of 76 (T.elliptica, Gazal Park-Tehran, Iran) was classified as group1B. Genotypes plot showed similar results regarding the parameters of A1 and A2 and Stebbins’ table symmetry classes. At the end, the proximity and remoteness of studied species was chromosomally identified and the results were presented as dendrogram.


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