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Study on Antimicrobial Resistance of Escherichia Coli o157:H7/NM isolated from Raw Bovine, Camel, Water Buffalo, Caprine and Ovine milk

Author Affiliations

  • 1School of Veterinary Medicine, Shiraz University, Shiraz, IRAN
  • 2 College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, IRAN

Res. J. Recent Sci., Volume 4, Issue (4), Pages 20-22, April,2 (2015)


Escherichia coli O157:H7 has appeared as a food-producing pathogen in public health. Previously the various patterns of E.coli‘s transition in bovine cattle have been mentioned such as long-term migration or transient cycle in host of bovine. The recognition of animals breeding E.coli, is difficult because some adult cattle do not appear signs of illness and shedding of it and they scatter diseases in everywhere. This study is aimed at determining antimicrobial resistance of Escherichia coli o157:H7/NM isolated from raw milk. The study was conducted on bovine, camel, water buffalo, carbine and ovine in Iran. According to Clinical and Laboratory Standards Institute (CLSI), 32 isolated milk experienced for susceptibility to antimicrobial drugs by Kirby-Bauer disk diffusion test using Muller-Hinton agar. Overall 26 cases of 32 E.coli isolates were resistant to one or more antimicrobial agents. Resistance to ampicillin was the most common finding (46.6%), and after that resistance to erythromycin (31.1%), gentamycin (9.4%), nalidixic acid (12.5%), doxycyclin (904%), streptomycine (9.4%), kanamycin(904%), tetracycline (9.4%), chlorampenicol (603%), and amoxicilin( 3.1%). All E. coli isolates were sensitiveness to cefuroxime.


  1. Abongo BO and Momba MNB., Prevalence and characterization of Escherichia coli O157:H7 isolates from meat and meat products sold in Amathole District, Eastern Cape Province of South Africa, Int J. Food. Microbiol., 26, 173-176 (2009)
  2. Byrne C, Erol I, Call J, Buege D, Kaspar CW, Hiemke C, Fedorka Cray P, Hermosillo J, Ball T, Wallace M, Handy M and Luchansky JB., Characterization of Escherichia coli O157:H7 from downer and healthy dairy cattle in the upper Midwest region of the United States, Appl. Environ. Microbiol, 69, 4683-4688 (2003)
  3. Caro I, Fernandez-Barata VM, Alonso-Liamazares A and Garcia-Armesto MR., Detection, occurrence and characterization of Escherichia coli O157: H7 from raw ewe’s milk in Spain, J. Food Prot., 69, 920-924 (2006)
  4. Chapman PA, Cerdan Malo AT, Ellin M, Ashton R and Harkin MA., Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK, Int J. Food. Microbiol.64, 139-150 (2001)
  5. Cízek A, Dolejská M, Novotná R, Haas D and Vyskocil M., Survey of Shiga toxigenic Escherichia coli O157 and drug-resistant coliform bacteria from in-line milk filters on dairy farms in the Czech Republic, J. Appl. Microbiol., 104, 852-860 (2007)
  6. Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Disk Susceptibility Testing. 9th ed. Approved Standard (Document M2-A9). Clinical and Laboratory Standards Institute, Wayne, USA, (2006)
  7. Dontorou C, Papadopoulou C, Filiousis G, Economou V, Apostolou U, Zakkas G, Salamoura A, Kansouzidou A and Levidiotou S., Isolation of Escherichia coli O157:H7 from foods in Greece, Int J. Food. Microbiol., 82, 273-279 (2003)
  8. Ferens WA and Hovde CJ., Escherichia coli O157:H7: animal reservoir and sources of human infection, Food borne Pathog. Dis., 8, 465-485 (2011)
  9. Franco A, Lovari S, Cordaro G, Di Matteo P, Sorbara L, Lurescia M, Donati V, Buccella C and Battisti A.,Prevalence and concentration of verotoxigenic Escherichia coli O157:H7 in adult sheep at slaughter from Italy, Zoonoses Public Health., 56, 215-220 (2009)
  10. Gannon VPJ, D’Souza S, Graham T, King RK, Rahn K and Read S., Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains, J. Clin. Microbiol., 35, 656-662 (1997)
  11. Goncuoglu M, Erol I, Ayaz ND, Ormanci FSB and Kaspar CW., Isolation and genomic characterization of Escherichia coli O157:H7 in bile of cattle, Ann. Microbiol., 60, 293-297 (2010)
  12. Jeong KC, Kang MY, Heimke C, Shere JA, Erol I and Kaspar CW., Isolation of Escherichia coli O157:H7 from the gall bladder of inoculated and naturally-infected cattle, Vet Microbiol., 119, 339-345 (2007)
  13. Lanz R, Kuhnert P and Boerlin P., Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland, Vet. Microbiol. 91, 73-84 (2003)
  14. Mead PS and Griffin PM., Escherichia coli O157:H7, Lancet., 352, 1207-1212 (1998)
  15. Oliver SP, Murinda SE and Jayarao BM., Impact of antibiotic use in adult dairy cows on antimicrobial resistance of veterinary and human pathogens: A comprehensive review, Food born Pathog. Dis., 8, 337-355 (2011)
  16. Paton AW and Paton JC., Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA , rfbO111, and rfbO157, J. Clin. Microbiol.,36, 598-602 (1998)
  17. Picozzi C, Foschiion R, Heuvelink A and Beumer R., Phenotypic and genotypic characterization of sorbitol-negative of slow-fermenting (suspected O157) Escherichia coli isolated from milk samples in Lombardy region, Lett. Appl. Microbiol., 40, 491-496 (2005)
  18. Schroeder CM, Zhao C, DebRoy C, Torcolini J, Zhao S, White DG, Wagner DD, McDermott PF, Walker RD and Meng J., Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine and food, Appl. Environ. Microbiol.,68, 576-581 (2002)
  19. Van Baale MJ, Sargeant JM, Gnad DP, DeBey BM, Lechtenberg KF and Nagaraja TG., Effect of forage or grain diets with or without monensin on ruminal persistence and fecal Escherichia coli O157:H7 in cattle, Appl. Environ. Microbiol., 70, 5336-5342 (2004)
  20. Walsh C, Duffy G, O'Mahony R, Fanning S, Blair IS and McDowell DA., Antimicrobial resistance in Irish isolates of verocytotoxigenic Escherichia coli (E. coli)-VTEC, Int J. Food.Microbiol., 109, 173-178 (2006)
  21. Wong CS, Jelacis S, Habeeb RL, Watkins SL and Tarr PL., The risk of the hemolytic-uremic syndrome after antibiotic treatment of Eschrichia coli O157:H7 infections, N. Engl. J. Med., 342, 1930-1936 (2000)