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Evaluation of Lenticular Reduced Glutathione, Ascorbic Acid and Lipid Peroxidation Levels among Cataractous Men

Author Affiliations

  • 1 Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641 043, Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 4, Issue (4), Pages 61-65, April,2 (2015)

Abstract

Reactive oxygen species which are produced through plethora of reactions during oxidative stress in the human lens may play an important role in the formation of cataract. Lipid peroxidation may cause exacerbation of lens tissue. Glutathione and ascorbic acid are among the vital lens antioxidants that are involved in scavenging free radicals. The rationale of the present research was to assess the levels of reduced glutathione, ascorbic acid and lipid peroxidation in the eye lens among cataractous subjects. The lens samples were obtained from the ophthalmic centres in and around Coimbatore. They were categorised into apparently normal cataract men (n=15), diabetic cataract men (n=15) and hypertensive cataract men (n=15). All the parameters were analysed spectrophotometrically. The data interpretation was performed by Kruskal Wallis test. The outcome of the study showed a significant difference (p0.05) in the levels of ascorbic acid and lipid peroxidation among apparently cataract men and diabetic cataract men. However, these groups did not show significant difference albeit they showed variations in the levels of glutathione. The levels of glutathione, ascorbic acid and lipid peroxidation showed no significant difference among diabetic cataract men and hypertensive cataract men. The levels of ascorbic acid were found to have significant difference (p0.05) among apparently normal cataract men and hypertensive cataract men whereas similar trend was not observed in the levels of glutathione and lipid peroxidation.

References

  1. Thylefors B. and Negrel A.D., Pararajasegaram R. and Dadzie K.Y., Global data on blindness, Bull. World. Health. Organ., 73(1), 115–121 (1995)
  2. Knudtson M.D., Klein B.E. and Klein R., Age - related eye disease, visual impairment and survival: the Beaver Dam Eye Study, Arch. Ophthalmol.,124(2), 243–249 (2006)
  3. Forooghian F., Agrón E., Clemons T.E., Ferris F.L.3rdand Chew E.Y., Visual acuity outcomes after cataract surgery in patients with age-related macular degeneration: age-related eye disease study report no. 27, Ophthalmol., 116(11), 2093–2100 (2009)
  4. Syam P.P., Eleftheriadis H., Casswell A.G., Brittain G.P., McLeod B.K. and Liu C.S., Clinical outcome following cataract surgery in very elderly patients, Eye., 18(1), 59–62 (2004)
  5. Tabin G., Chen M. and Espandar L., Cataract surgery for the developing world, Curr. Opin. Ophthalmol., 19(1), 55–59 (2008)
  6. Lu M., Cho E., Taylor A., Hankinson S.E., Willett W.C. and Jacques P.F., Prospective study of dietary fat and risk of cataract extraction among US women, Am. J. Epidemiol., 161(10), 948–959 (2005)
  7. Bojarskiene F., Cerniauskiene L.R., Paunksnis A. and Luksiene D.I., Association of metabolic syndrome components with cataract, Medicina (Kaunas).,42(2), 115–122 (2006)
  8. Sacca S.C, Roszkowska A.M. and Izzotti A., Environmental light and endogenous antioxidants as the main determinants of non-cancer ocular diseases, Mutat. Res., 752(2), 153–171 (2013)
  9. Williams D.L., Oxidation, antioxidants and cataract formation: a literature review, Vet. Ophthalmol., 9(5), 292-298 (2006)
  10. Fletcher A.E., Free radicals, antioxidants and eye diseases: evidence from epidemiological studies on cataract and age-related macular degeneration, Ophthalmic. Res., 44(3), 191-198 (2010)
  11. Spector A., Oxidative stress-induced cataract: mechanism of action, FASEB. J., 9(12), 1173–1182 (1995)
  12. Sacca S.C., Bolognesi C., Battistella A., Bagnis A. and Izzotti A., Gene-environment interactions in ocular diseases, Mutat. Res.,667(1-2), 98–117 (2009)
  13. Babizhayev M.A., Biomarkers and special features of oxidative stress in the anterior segment of the eye linked to lens cataract and the trabecular meshwork injury in primary open-angle glaucoma: challenges of dual combination therapy with N-acetylcarnosine lubricant eye drops and oral formulation of nonhydrolyzed carnosine, Fundam. Clin. Pharmacol.,26(1), 86–117 (2012)
  14. Moron M.S., Depierre J.W. and Mannervik B., Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver, Biochim. Biophys. Acta., 582(1), 67-78 (1979)
  15. Omaye S.T, Turnbull J.D. and Sauberlich H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids, Methods. Enzymol., 62, 3-11 (1979)
  16. Niehaus W.G Jr. and Samuelsson B., Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation, Eur. J. Biochem.,6(1), 126-130 (1968)
  17. Xie P.Y., Kanai A., Nakajima A., Kitahara S., Ohtsu A. and Fujii K., Glutathione and glutathione-related enzymes in human cataractous lenses, Ophthalmic. Res., 23(3), 133–140 (1991)
  18. Linetsky M., Shipova E., Cheng R. and Ortwerth B.J., Glycation by ascorbic acid oxidation products leads to the aggregation of lens proteins, Biochim. Biophys. Acta., 1782(1), 22–34 (2008)
  19. Tarwadi V.K., Chiplonkar S.A. and Agte V, Dietary and nutritional biomarkers of lens degeneration, oxidative stress and micronutrient inadequacies in Indian cataract patients, Clin. Nutr., 27(3), 464–472 (2008)
  20. Varma S.D. and Richards R.D., Ascorbic acid and the eye lens, Ophthalmic Res.,20(3), 164–173 (1988)
  21. Varma S.D., Srivastava V.K. and Richards R.D., Photoperoxidation in lens and cataract formation: preventive role of superoxide dismutase, catalase and vitamin C, Ophthalmic Res., 14(3), 167–175 (1982)
  22. Bevan R.J., Mistry N., Patel P.R., Halligan E.P., Dove R. and Lunec J., Can vitamin C induce nucleotide excision repair? Support from in vitro evidence, Br. J. Nutr., 103(5), 686–695 (2010)
  23. Tessier F., Moreaux V., Birlouez-Aragon I., Junes P. and Mondon H., Decrease in vitamin C concentration in human lenses during cataract progression., Int. J. Vitam. Nutr. Res., 68(5), 309–315 (1998)
  24. Barnett M., Lin D., Akoyev V., Willard L. and Takemoto D., Protein kinase C epsilon activates lens mitochondrial cytochrome c oxidase subunit IV during hypoxia, Exp. Eye. Res., 86(2), 226–234 (2008)
  25. Shui Y.B. and Beebe D.C., Age-dependent control of lens growth by hypoxia, Invest. Ophthalmol. Vis. Sci., 49(3), 1023–1029 (2008)