International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

HPLC phytochemical analysis and antiproliferative activity of n-butanol fraction of Solanum aethiopicum L. (Solanaceae) from Côte d’Ivoire

Author Affiliations

  • 1Laboratoire de Chimie Bio Organique et de Substances Naturelles (LCBOSN), UFR-SFA, Universit Nangui Abrogoua, 02 BP 0801 Abidjan 02,COTE DIVOIRE
  • 2 Biomedical Laboratory Science and Technology, Department of Biomedical Sciences, Health and Society, Malm University, SE-205 06 Malm,SWEDEN

Res. J. Recent Sci., Volume 4, Issue (12), Pages 81-87, December,2 (2015)


Solanum aethiopicum L. (Solanaceae) commonly called Aubergine, is a popular plant and one of the most eaten as a leaf and vegetable. Due to the lack of phytochemical and biological information on the species of Cte d'Ivoire, the goal of our previous investigations was mainly a qualitative analysis by TLC and anti-hypertensive activity of organic extracts from the flower stalk. This research has highlighted the richness of the plant in different secondary metabolites and an efficient in vivo hypotensive activity. The aim of the present work was to certify by HPLC analyzes, the phytochemical composition of n- butanol fraction (F4) of S. aethiopicum flower stalk obtained from the methanol extract and study its anticancer activity on Jurkat cell lines. The results have confirmed our previous findings in relation to the diversity of bioactive phytocompounds contained in S. aethiopicum flower stalk; on one hand, Quercetin identified in F4 at a retention time of 2.65 min and on the other hand, F4 induces in vitro antiproliferative and apoptotic activities on Jurkat cells with IC50= 11.77 2.4 mg/ml.


  1. Stphane Q., Deffieux D., Douat-Casassus C. and Laurent P., Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis, Angew. Chem., (50), 586-621 (2011)
  2. Lock K., Pomerleau J., Causer L., Altman D.R. and McKee M., The global burden of disease attributable to low consumption of fruit and vegetables: implications for the global strategy on diet, Bull World Health Organ., (83), 100-108 (2005)
  3. Fraga C.G., Plant polyphenols: how to translate their in vitro antioxidant actions to in vivo conditions, IUBMB Life, (59), 308-315 (2007)
  4. Daunay M.C., Eggplant In Handbook of Plant Breeding; Prohens, J., Nuez, F., Springer, N Y.(2), 163-220 (2008)
  5. Yekeen T.A., Studies on the proximate analysis of Solanum aethiopicum, Lactuca taraxacifolia and Talinum triangulare and potential cytotoxic effects of their aqueous extract using Allium cepa assay, Annals of Biological Research, 2(5), 696-706 (2011)
  6. Shalom N.C., Abayomi C.O., Okwuchukwu K.E., Opeyemi C.E., Olajumoke K.A. and Damilola I.D., Proximate and Phytochemical Analyses of Solanum aethiopicum L. and Solanum macrocarpon L. Fruits. Res. J. Chem. Sci., 1(3), 63-71 (2011)
  7. Mamyrbekova-Bekro J.A., Boua B.B., K.K. Christian, Bkro Y.A., Qualitative and pharmacological analysis of 2 antihypertensive plants used at Ngramanssabo in Cte dIvoire, Nature & Technologie, 11 (2012)
  8. Ladiguina E.Y., Safronitch L.N., Otriachenkova V.E., Balandina I.A. and Grinkevitch N.I., Chemical analysis of medicinal plants, Edition Masson, Paris (France), 1879 (1983)
  9. Bkro YA., Mamyrbekova-Bekro J.A., Boua B.B., Tra bi F.H. and Ehile E.E., Ethnobotanic and phytochemical screening studies of Caesalpinia benthamiana (Baill.) Herend. & Zarucchi (Caesalpiniaceae), Sciences & Nature, (4), 217-225 (2007)
  10. Zahra A., Ramesh M. and Sima Y.,Cytotoxic Effect of Aqueous and Alcoholic Extracts of Pterocarya fraxinifolia Leaves on K562 Cell Line, Zahedan J Res Med Sci., 16(3), 1-5 (2014)
  11. Hae J. W., Chang H. H., Young H. K., Hyun J. K., Byung W.K., Jae S. C. and Kwang H.K., Induction of apoptosis in human acute leukemia Jurkat T cells by Albizzia julibrissin extract is mediated via mitochondria-dependent caspase-3 activation, J. of Ethnopharmacology, 106(3), 383-389 (2006)
  12. HwangYJ., LeeEJ., KimHR andHwangKA.In vitro antioxidant and anticancer effects of solventfractions fromprunella vulgarisvar. lilacina, BMC Comp. and Alter. Medicine, (13), 310 (2013)
  13. Niranga H. N., Alex J., Rao J.V., Mathew J.A. and Udupa N., In vitro anti-cancer and anti-oxidant activity of different fractions of Diospyros peregrine unripe fruit extract. Free Rad. and Antioxidants, 12(4), 45-49 (2012)
  14. Sebastian B., Johan E., Krister T. and Emma S., A water gradient can be used to regulate drug transport across skin, Journal of Controlled Release, (143), 191-200 (2010)
  15. Schneider U., Schwenk H., Bornkamm G., "Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma", Int J. Cancer, 19(5), 621- 6 (1977)
  16. Shanmugam R., Gowthamarajan K., Priyanka D.L., Madhuri K., Hemnath E. and Dhanabal S.P., Development and Validation of a RP- UFLC Method for Simultaneous Estimation of Quercetin and Rutin, Hygeia.J.D.Med., 5 (1),113-120 (2013)
  17. Jelena S.S., Dejan Z.M. and Jelena B.Z., Benzophenone Suppression of Quercetin Antioxidant Activity towards Lipids under UV-B Irradiation Regime: Detection by HPLC Chromatography, Journal of Chemistry, 9 (2013)
  18. Timbola A.K., Souza D.C., Giacomelli C., Spinell A., Electrochemical Oxidation of Quercetin in Hydro-Alcoholic Solution, J. Braz. Chem. Soc., 17(1), 139-148 (2006)
  19. umari A., Yadav S.K., Pakade Y.B., Singh B., Yadav S.C., Development of biodegradable nanoparticles for delivery of quercetin, Colloids Surf B Biointerfaces, 80(2),184 -192 (2010)
  20. El-Sayed S., Hameed A., Bazaid S.A. and Salman S.M., Characterization of the Phytochemical Constituents of Taif Rose and Its Antioxidant and Anticancer Activities, Bio Med Res. Inter.,13 (2013)
  21. Akowuah G.A., Sadikun A. and Mariam A., Flavonoid Identification and Hypoglycaemic Studies of the Butanol Fraction from Gynura procumbens, Pharmaceutical Biology, 40(6), 405-410 (2002)