International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Vulvovaginal Candidiasis: Isolation and Identification of Candida from Reproductive Age group Woman

    ,

Author Affiliations

  • 1 Shree Ramkrishna Institute of Computer Education and Applied Sciences, Veer Narmad South Gujarat University, Surat-395 001, Gujarat, INDIA

Res. J. Recent Sci., Volume 3, Issue (IVC-2014), Pages 30-33,(2014)

Abstract

Vulvovaginal candidiasis (VVC) is an oppertunistic mucosal infection caused by varies candida species that affects large number of healthy women of childbearing age. It is estimated that 75% of women will experience at least one episode in their lifetime. candida albicans is a dimorphic commensal organism of urogenital tract and has been identified as main pathogenic agent in VVC, accounting for approximately 85-90% of patient with positive cultures. A prospective study of female genital swabs collected from gynecological clinic and analysed for microscopy, culture, speciallized test. Most common VVC caused by candida albicans and non albican species were also detected like candida tropicalis, candida glabrata in 50 samples collected from pregnant and non pregnant women. Microscopic analysis was performed by 10% KOH wet preperation technique and gram staining. Sabouraud dextrose broth was used as primary growth medium and than candida species were identified by growth on HiCrome candida speciallized medium. further identification was done by special test- germ tube formation, chlamydospore production test, carbohydrate assimilation and fermentation test.

References

  1. J.D. Sobel, Vaginitis, New England Journal of Medicine, 337(26), 1896–1903 (1997)
  2. J.D. Sobel, Candida vulvovaginitis, Clinical Obstetrics and Gynecology, 36, 153–212, (1997)
  3. A.M. Tortorano, J. Peman and H. Bernhardt et al., Epidemiology of candidaemia in Europe: results of 28-month European Confederation of Medical Mycology (ECMM) hospital-based surveillance study, European Journal of Clinical Microbiologyand Infectious Diseases, 23(4), 317–322, (2004)
  4. M.Y¨ucesoy and S. Marol, Performance of CHROMAGAR candida and BIGGY agar for identification of yeast species, Annals of Clinical Microbiology and Antimicrobials, article 8, (2003)
  5. B. Sendid, N. Franc¸ois and A. Standaert et al., Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species, Journal of Medical Microbiology, 56(4), 495–499 (2007)
  6. Soll D.R., High-frequency switching in Candida albicans,” Clinical Microbiology Reviews, 5(2), 183–203, (1992)
  7. Williamson M.I., Samaranayake L.P. and MacFarlane T.W., Biotypes of oral Candida albicans and Candida tropicalis isolates, Journal of Medical and Veterinary Mycology, 24(1), 81–84 (2001)
  8. Zdolsek B., Hellberg D. and Froman G., et al., Culture and wet smear microscopy in the diagnosis of low-symptomatic vulvovaginal candidosis, Eur J Obstet Gynecol Reproduct Biol, 58, 47–51 (1995)