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Influence of Permeation enhancer on Ethosomes bearing Lamivudine for Transdermal Drug Delivery

Author Affiliations

  • 1Smriti College of Pharmaceutical Education, Indore, INDIA
  • 2 Department of Pharmaceutics, NGSMIPS, Mangalore, INDIA

Res. J. Recent Sci., Volume 3, Issue (IVC-2014), Pages 155-160,(2014)

Abstract

The application of medicinal substances to the skin is a concept undoubtedly as old as humanity, the Papyrus records of ancient Egypt describes a variety of such medication for external use. Innovative techniques for drug delivery have been explored in human medicine in recent years. Transdermal drug delivery has gained much vital impact in dosage form; it has been impending as asubstitute to oral delivery. Several tactics for improving transdermal drug delivery were researched; Ethosome are ethanol derivatives of liposomes. Permeation enhancer has the ability to cause the perturbation of the skin and help in drug penetration. The ethanol effect of ethosome and permeation enhancer will synergistically enhance the drug penetration to deeper skin and systemic circulation. Lamivudineethosomal formulations were prepared and characterized for entrapment efficiency, fluorescence skin study and ex-vivo study. Propylene glycols (PG), oleic acid are penetration enhancer for lamivudine into skin. Ethosomes were prepared with propylene glycol (ETP) and linoleic acid (ETL) respectively. Ethosomes bearing propylene glycol and oleic acid has shown good flux values 1.5 fold and 2 fold than ethosomes (ET) respectively. The highest entrapment efficiency of ETP-2 is 64.6±1.5% and ETO-2 formulation is 68.2±4.1%. Increases in the concentration of both permeation enhancers decrease the entrapment efficiency. The fluorescence skin ex-vivo study of the ethosomes formulation reveals that oleic acid ethosomes has deeper penetration in the skin compared to ethosomes containing propylene glycol. The release of the ETP-2 and ETO-2 has both shown sustain release pattern for 12 hrs with reduced in lag time. Ethosomes containing oleic acid has better entrapment, enhancement of lamivudine penetration to deeper skin compares ethosomes containing propylene glycol.

References

  1. John D. Mullins., Medicated Application, Remington Pharm Sci, 16th edition, Mack publishing Company Eastern, Pennsylvania,(87),1518-1519 (1980)
  2. Sudhakar C.K., Upadhyay N. Jain S. and Charyulu R.N., Ethosomes as Non-invasive Loom for Transdermal Drug Delivery In: Sebastian M., Ninan N., Haghi A.K. editors Nanomedicine and Drug Delivery. San Diego, Apple Academic Press, (1), 1-16 (2012)
  3. Evangeline D.D., Reddy Y.R., Kumar A.B., Formulation and evaluation of topical paste with honey mixture,Int J Pharm Pharm Sci, 3(5), 449-451 (2011)
  4. Prausnitz M.R., Langer R., Transdermal Drug Delivery, Nat Biotechnol,26(11), 1261–1268 (2008)
  5. Khalili M., Akbarzadeh A., Chiani M. and Torabi S., The effect of Nanoliposomal and PEGylated Nanoliposomal Forms of 6-Gingerol on Breast Cancer Cells. Res. J. Recent Sci., 2(5), 29-33, (2013)
  6. Ariponnammal S., Spectroscopic Analysis of Siddha Medicine NandukkalParpam, Res.J.Recent Sci., 1(5), 59-61, (2012)
  7. Guy R.H, Hadgraft J., New York: Marcel Dekker; Transdermal Drug Delivery (2003)
  8. Williams A., London: Pharmaceutical Press; Transdermal and Topical Drug Delivery(2003)
  9. Prausnitz M.R., Mitragotri S. and Langer R., Current status and future potential of transdermal drug delivery, Nat Rev Drug Discov, 3, 115–124 (2004)
  10. Mudigoudra B.S., Masti S.P. and Chougale R.B., Thermal Behavior of Poly (vinyl alcohol)/ Poly (vinyl pyrrolidone)/ Chitosan Ternary Polymer Blend Films, Res.J.Recent Sci., 1(9), 83-86 (2012)
  11. Bronaugh R.L. and Maibach H.I., editors. Edn. 4th. New York: Marcel Dekker; Percutaneous Absorption. (2005)
  12. Honeywell-Nguyen L.P. and Bouwstra J.A., Vesicles as a tool for transdermal and dermal delivery, Drug Discovery Today: Technologies, 2(1) 67-74, (2005)
  13. Verma P. and Pathak K., Therapeutic and cosmeceutical potential of ethosomes: An overview, J Adv Pharm Technol Res; 1(3): 274–282.(2010)
  14. Elsayed M.A., Abdallah Y.O., Naggar F.V. and Khalafallah N.M., Lipids vesicles for skin delivery of drugs: Reviewing three decades of research, Int J Pharm332,1–16, (2006)
  15. Kalbitz J., Neubert R. and Wohlrab W., Modulation of drug penetration in the skin, Pharmazie,51, 619–637, (1996)
  16. Trommer H. and Neubert R.H., Overcoming the Stratum Corneum: The Modulation of Skin Penetration. Skin Pharmacol Physiol, 19, 106–121, (2006)
  17. Hadgraft J., Modulation of the barrier functions of the skin, Skin PharmacolAppl Skin Physiol,14(suppl 1), 72–81, (2001), 155-160 (2014)
  18. Naik A., Pechtold L., Potts O. and Guy R.H., Mechanism of oleic acid-induced skin penetration enhancement in vivo in humans, J Control Release, 37, 299-06, (1995)
  19. Coderch L., Lopez O., Maza A. and Parra J.L., Ceramides and skin function, Am J Clin Dermatol, 4(2), 107-29 (2003)
  20. Wartewig S., Neubert R., Rettig W., Hesse K., Structure of stratum corneum lipids characterized by FT-Raman spectroscopy and DSC. IV. Mixtures of ceramides and oleic acid, Chem. Phys. Lipids, 91, 145–152, (1998)
  21. Rowat A.C., Kitson N. and Thewalt J.L., Interactions of oleic acid and model stratum corneum membranes as seen by H NMR, Int J Pharm, 307(2), 225-3, (2006)
  22. Phillips C.A., Michniak B.B., Transdermal delivery of drugs with differing lipophilicities using azone analogs as dermal penetration enhancers, Journal of Pharmaceutical Sciences, 84(12), 1427-1433,(1995)
  23. Williams A.C. and Barry B.W., Penetration enhancers, Adv. Drug Deliv.Rev., 56, 603–618, (2004)
  24. Genina E.A., Bashkatov A.N., Larin K.V. and Tuchin V.V., Light–Tissue Interaction at Optical Clearing, in Laser Imaging and Manipulation in Cell Biology (ed F. S. Pavone), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 115-164, (2010)
  25. Jain S., Tiwary A.K., Sapra B. and Jain N.K., Formulation and evaluation of ethosomes for transdermal delivery of lamivudine, AAPS Pharm Sci Tech, 8(4), E111, (2007)
  26. Raffat S.K., Siddiqui M.S., Siddiq M., Shaikh Z.A. and Memon A.R.., HBVO: Human Biological Viruses Ontology, Res.J.Recent Sci., 1(10), 45-50, (2012)
  27. Upgade A., Bhaskar A., Issar S. and Senthamarai S.V., In Silico Charecterization of Keratitis Causing Herpes Simplex Virus (HSV-1) Membrane Proteins using Computational Tools and Servers, Res.J.Recent Sci., 1(11),27-31, (2012)
  28. Touitou E., Dayan N., Bergelson L., Godin B. and Eliaz M., Ethosomes-novel vesicular carrier for enhanced delivery characterization and skin penetration properties, J. cont Release, 65, 403-18, (2000)
  29. Chandran S.C., Shirwaikar A., Kuriakose M.R. and Sabna N., Development and evaluation of ethosomes for transdermal delivery of Fluconazole, Journal of Chemical, Biological and Physical Sciences, 2(1), 254-260, (2012)
  30. Panwar P., Pandey B., Lakhera P.C. and Singh K.P., Preparation, characterization, and in vitro release study of albendazole-encapsulated nanosize liposomes, Int J Nanomedicine, 5, 101-8 (2010)
  31. Aliasagar S. and Misra A., Studies in topical application of niosomally entrapped Nimesulide, J Pharm pharmaceut Sci., 5(3), 220-225 (2005)
  32. Jain S., Sapre R., Tiwary A.K. and Jain N.K., Proultraflexible Lipid Vesicles for Effective Transdermal Delivery of Levonorgestrel: Development, Characterization and Performance Evaluation, AAPS Pharm Sci Tech, 6 (3), E513-E522, (2005)
  33. Naik A., Pechtold L.A., Potts R.O. and Guy R.H., Mechanism of oleic acid-induced skin penetration enhancement in vivo in humans, J Control Release, 37,299-06 (1995)
  34. Vlietinch A.J., Bruyne T.D, Berghe D.V., Plant substances As Antiviral agents/ In; Hostettmann, Current Organic chemistry, 1(4), 330-331, (1997)
  35. http://www.lipoid.com/en/phospholipids Accessed on June 10, (2014)
  36. Mast R., Function of glycerine in cosmetics, Inedited by Jungermann E, Sontag NOV. Glycerine: a key cosmetic ingredient. Marcel Dekker, New York, 260-261, (1991)
  37. Alam E.A., Initiation of Pharmaceutical Factories depending on more Application of Biotechnology on some Medicinal Plants Review Article (In Vitro Production of some Antioxidant, Analgesic, Antibacterial, Antidiabetic agents), Res.J.Recent.Sci., 1(ISC-2011), 398-404, (2012)
  38. Annapureddy M.R., Nelavalli S., Ramadoss K. and Srinivasababu P., Dendritic Architechture for the Delivery of Levofloxacin against Resistant Producing Strains, Res. J. Pharmaceutical Sci, 1(1), 6-9, (2012)