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Screening of Raw Buffalo's Milk from Karnataka for Potential Probiotic Strains

Author Affiliations

  • 1insNannu Shafakatullah* and M. Chandra Dept. of Bio-Sciences, Mangalore University, Mangalagangothri-574199, Mangalore, Karnataka, INDIA

Res. J. Recent Sci., Volume 3, Issue (9), Pages 73-78, September,2 (2014)


Buffalo’s milk was cultured with appropriate dilution on MRS media for the isolation of potential probiotics and pure cultures were obtained by sub-culturing. Purification of cultures were confirmed by Gram’s staining and catalase test and identified based on morphological, cultural, physiological and different biochemical characteristics as presented in Bergey’s Manual of Systematic Bacteriology. During lactic acid bacterial (LAB) transit through the gastrointestinal tract, ingested microorganisms were exposed to successive stress factors, including low pH in the human stomach and bile salt. These isolates were examined for survival in bile salt, acidic pH, different NaCl concentrations, anti pathogenic activity, as well as survival at different storage temperatures. These stress factors can be used as criteria for the evaluation of probiotic strains. Isolated strains of Lactobacilli spp. and Bifidobacterium spp. showed satisfactory probiotic potentials.


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