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Potential of Endophytic Bacteria Isolated from Vitex negundo L. to Produce Auxin

Author Affiliations

  • 1Dept. of Microbiology, Ramnarain Ruia College, Matunga, Mumbai 400019, INDIA

Res. J. Recent Sci., Volume 3, Issue (8), Pages 38-42, August,2 (2014)


Endophytic bacteria with plant hormone producing ability were screened. Bacillus endophytes were isolated from the leaves of Vitex negundo L. (nirgundi). The ability of these strains to synthesize auxin (indole-3-acetic acid) was demonstrated. Indole-3-acetic acid (IAA) was extracted from the endophytes. IAA production by these endophytes was established by spectrophotometry (42g/ml) and rapid in situ assay. IAA production was further confirmed by thin-layer chromatography (TLC) as well as by high-performance liquid chromatography (HPLC). The released IAA increased with the increase of the initial concentration of L-tryptophan in the medium. Effect of auxin producing endophytes on plant growth was demonstrated by conducting pot assay. These endophytic strains showed plant growth promoting characteristics and also exhibited obvious promoting effects on Phaseolus vulgaris. Plant associated endophytes with the ability to produce phytohormone can be valuable biological resources and have vast agricultural potential.


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