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Studies for Identification of Polymorphism in Promoter Sequence of growth hormone gene from microminipig (MMP) with direct sequencing PCR

Author Affiliations

  • 1Laboratory of Molecular Cell Physiology, Department of Applied Bioresources, Faculty of Agriculture, Ehime University, Matsuyama-790-8566,JAPAN
  • 2Fuji Nojo Service, 5247-34 Kitayama, Fujinomiya, Shizuoka, 418-0012, JAPAN
  • 3Venture Business Laboratory, Ehime University, Matsuyama-790-8577, JAPAN

Res. J. Recent Sci., Volume 3, Issue (12), Pages 1-8, December,2 (2014)


Growth hormone (GH) is the main protein hormone released from anterior pituitary gland of hypothalamus region of brain in mammals. It is extensively studied in pig and it is encoded by GH gene. Microminipig (MMP) is the world’s smallest pig developed by Fuji Micra Inc, Japan. It weighs 6-7 kg at the age of 6 months. The transcription regulation is the key step in expression of eukaryotic gene expression and it depends on various cis and transacting elements. To our knowledge, the variation in sequence of GH promoter region in MMP, 1 kb upstream from 1st exon has studied first time. All consensus sites are conserved in MMPs except TATA box sequence which has another version such as TATATAAA. The Variations at -934, -713, -626, -574 and -499 sites are observed only in MMPs. These variations of MMPs could be used as possible genetic markers for pig production.


  1. Evans H.M. and Long J. A., Characteristic effects upon growth, oestrus and ovulation induced by the intraperitoneal administration of fresh anterior hypophyseal substance, Anatomical Record,23, 19 (1922a)
  2. Evans H.M. and Long J.A., Characteristic effects upon growth, oestrus and ovulation induced by the intraperitoneal administration of fresh anterior hypophyseal substance, Proceedings of the National Academy of Sciences USA, , 38–39 (1922b)
  3. Evans H.M. and Simpson M.E., Hormones of the anterior hypophysis, American Journal of Physiology,98, 511–546 (1931)
  4. Lee M.O. and Schaffer N.K., Anterior pituitary growth hormone and the composition of growth, The Journal of Nutrition,7, 337–363 (1934)
  5. Li C.H., Evans H.M. and Simpson M.E., Isolation and properties of the anterior hypophyseal growth hormone, Journal of Biological Chemistry, 159, 353–366 (1945)
  6. Kostyo J.L., Rapid effects of growth hormone on amino acid transport and protein synthesis, Annals of the New York Academy of Sciences, 148, 389–407 (1968)
  7. Scanlon M.F., Issa B.G. and Dieguez C., Regulation of growth hormone secretion, Hormone Research, 46, 149-154 (1996)
  8. Yerle M., Mansais Y., Thomsen PD. and Gellin J., Localization of the porcine growth hormone gene to chromosome 12p1.2--�p1.5. Animal Genetics,24, 129-131 (1993)
  9. Vize P.D. and Wells J.R., Spacer alterations which increase the expression of porcine growth hormone in E. coli., FEBS Letters, 213, 155-158 (1987)
  10. Kaneko N., Itoh K., Sugiyama A. and Izumi Y., Microminipig, a non-rodent experimental animal optimized for life science research : Preface. 2011, Journal of Pharmacological Sciences, 115, 112–114 (2011)
  11. Becker P.B., Ruppert S. and Schutz G., Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors, Cell, 51, 435-443 (1987)
  12. Baeuerle P.A. and Baltimore D., Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor, Cell, 53, 211-217 (1988)
  13. Schirm S., Jiricny J. and Schaffner W., The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity, Genes and Development, , 65-74 (1987)
  14. Ondek B., Shepard A. and Herr W., Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities, EMBO (European Molecular Biology Organization) Journal, , 1017-25 (1987)
  15. Serfling E., Jasin M. and Schaffner W., Enhancers and eukaryotic gene transcription, Trends in Genetics,, 224-230 (1985)
  16. Dynan W.S. and Tjian R., Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins, Nature 316, 774-778 (1985)
  17. Maniatis T., Goodbourn S. and Fischer J.A., Regulation of inducible and tissue-specific gene expression, Science,236, 1237-1245 (1987)
  18. Ptashne M., How eukaryotic transcriptional activators work, Nature, 35, 683-689 (1988)
  19. Lillie J.W. and Green M.R., Gene transcription : Activator's target in sight, Nature,341, 279-280 (1989)
  20. Ma J. and Ptashne M., Converting a eukaryotic transcriptional inhibitor into an activator, Cell, 55, 443-446 (1988)
  21. Sturm S., Tanaka M. and Herr W., The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16, Nature,341, 624-630 (1989)
  22. Theill L.E. and Karin M., Transcriptional control of GH expression and anterior pituitary development, Endocrine Reviews,14, 670-689 (1993)
  23. Faisst S. and Meyer S., Compilation of vertebrate-encoded transcription factors, Nucleic Acids Research, 20, 3-26 (1992)
  24. Kim J.L., Nikolov D.B. and Burley S.K., Co-crystal structure of TBP recognizing the minor groove of a TATA element, Nature, 365, 520-527 (1993)
  25. Kim Y., Geiger J.H., Hanh S. and Sigler P.B., Crystal structure of a yeast TBP/TATA-box complex, Nature,365, 512-520 (1993)
  26. Kirkpatrick B.W., Huff B.M. and Casas-Carrillo E., Double-strand DNA conformation polymorphisms as a source of highly polymorphic genetic markers, Animal Genetics, 24, 155- 161 (1993)
  27. Castrillo J.L., Theill L.E. and Karin M., Function of the homeodomain protein GHF1 in pituitary cell proliferation, Science, 253, 197–199 (1991)
  28. Mangalam H.J., Albert V.R., Ingraham H.A., Kapiloff M., Wilson L., Nelson C., Elsholtz H. and Rosenfeld M.G., A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally, Genes and Development, , 946–958 (1989)
  29. McCormick A., Brady H., Fukushima J. and Karin M., The pituitary-specific regulatory gene GHF1 contains a minimal cell type-specific promoter centered around its TATA box, Genes and Development,, 1490-1503 (1991)
  30. Lemaigre F.P., Courtois S.J., Lafontaine D.A. and Rousseau G.G., Evidence that the upstream stimulatory factor and the Spl transcription factor bind in vitro to the promoter of the human-growth-hormone gene, European Journal of Biochemistry,181, 555-561 (1989)
  31. Larsen N.J. and Nielsen V.H., DNA sequence variation in the porcine growth hormone promoter region from Danish and exotic pigs, Animal Biology, , 151-166 (1997)
  32. Schaufele F., West B.L. and Reudelhuber T.L., Overlapping Pit-1 and Spl binding sites are both essential to full rat growth hormone gene promoter activity despite mutually exclusive Pit-1 and Spl binding, The Journal of Biological Chemistry, 265, 17189-17196 (1990)
  33. Lipkin S.M., Naar A.M., Kalla K.A., Sack R.A. and Rosenfeld M.G., Identification of a novel zinc finger protein binding a conserved element critical for Pit-1-dependent growth hormone gene expression, Genes and Development, 1674-1687 (1993)
  34. Courtois S.J., Lafontaine DA, Lemaigre FP, Durviaux SM. and Rousseau GG., Nuclear factor-I and activator protein-2 bind in a mutually exclusive way to overlapping promoter sequences and trans-activate the human growth hormone gene, Nucleic Acids Research, 18, 57-64 (1990)
  35. Schaufele F., Cassill J.A., West B.L. and Reudelhuber T., Resolution by diagonal gel mobility shift assays of multisubunit complexes binding to a functionally important element of the rat growth hormone gene promoter, The Journal of Biological Chemistry, 265,14592-14598 (1990)