International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Beneficiary Effect of Arbuscular Mycorrhiza to Trigonella Foenum-Graceum in Contaminated Soil by Heavy Metal

Author Affiliations

  • 1 Environmental Sciences Research Laboratory, Department of Botany, Wilson College, Mumbai, INDIA

Res. J. Recent Sci., Volume 2, Issue (ISC-2012), Pages 29-32, February,2 (2013)


Because of industrialization and urbanization, there is no much land is available for urban farming in and around Mumbai. Wherever the small lands are available as open space, unused lands, barren lands etc are contaminated by heavy metals which come through industrial waste disposal. Such lands can be mycoremediated by use of mycorrhizal fungi to a certain extent and can be utilized for urban farming of leafy vegetables. Present investigation was carried out in the form of pot experiment to check the response of Glomus mosseae to Trigonell foenum-graceum which was grown in soil contaminated with heavy metal Arsenic. During these experiments, soils with different concentrations of arsenic with and without mycorrhizal inoculums were tested in Trigonella foenum – graceum. The response of mycorrhiza in T. foenum-graceum was determined in terms of percentage germination of seeds, sustainability of seedlings, fresh weight and dry weight of plants etc. It was observed that in the pot with soil contaminated with arsenic and no mycorrhizal inoculum, performance was very bad in terms of all aspects of growth, whereas in the pot where mycorrhizal inoculum was added along with contaminated soil, the performance of the plant was better. The pot showing no contaminated soil with arsenic but the inoculum of mycorrhiza was showing best results in terms of percentage germination of seeds, sustainability of seedlings, fresh weight and dry weight of plants.


  1. Ghosh M. and Singh S.P., A review on phytoremediation of heavy metals and utilization of its by-products Applied Ecological Environmental Research, 3(1), 1–8 (2005)
  2. Smith S.E. and D.J. Read, Mycorrhizal Symbiosis Academic press, San Diego, USA (1997)
  3. Gaur A. and Adholeya A. Prospect of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils, Current Science, 86, 528 (2004)
  4. Fayuan Wang, Xiangui Lin and Rui Yin Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil, Plant and Soil Sciences, 269, 225-232 (2005)
  5. Ultra V. U. Jr., E. S. Tanaka, E. K. Sakurai and E. K. Iwasaki Effects of arbuscular mycorrhiza and phosphorus application on arsenic toxicity in sunflower (Helianthus annuus L.) and on the transformation of arsenic in the rhizosphere, Plant soil, 290, 29–41 (2007)
  6. Weissenhorn I., Leyval C., Belgy G. and Berthelin J., Arbuscular mycorrhizal contribution to heavy metal uptake by maize (Zea mays L.) in pot culture with contaminated soil. Mycorrhiza 5, 245–251(1995)
  7. Gerdeman J.W. and T.H., Nicolson Spores of mycorrhizal Endogene sp. extracted from soil by Wet-sieving and decanting, Trans. Br. Mycol. Soc., 46(2), 235-244 (1963)
  8. Koske R.E., A modified procedure for staining roots to detect VAM fungi, Mycol. Res, 92(4), 486-488l (1989)
  9. Carol Grace and D.P. Stribley A safer procedure for routine staining of vesicular – arbuscular mycorhizal fungi, Mycol. Research, 95 (10), 1160-1162 (1991)
  10. Nicolson Nicolson’s formula Mycorrhiza News, 12(2), July 2000 (1955)