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Solubilisation of Uganda low grade Rock Phosphate by Pseudomonas fluorescence

Author Affiliations

  • 1PG Dept of Environmental Sciences, Sambalpur University, Jyotivihar, Odisha, INDIA
  • 2 Environment Cell, R and D, Hindalco Industries Limited, Hirakud, Sambalpur, Odisha, INDIAI
  • 3 nstitute of Minerals $ Materials Technology(CSIR), Bhubaneswar, Odisha, INDIA

Res. J. Recent Sci., Volume 2, Issue (ISC-2012), Pages 250-254, February,2 (2013)

Abstract

Most agricultural soils contain large reserves of phosphorus (P), a considerable part of which accumulates as a consequence of regular applications of P fertilizers. However, a greater part of soil phosphorus, approximately 95–99% is present in the form of insoluble phosphates and hence cannot be utilized by the plants. In the present study phosphate solubilising activity of Pseudomonas fluorescence against three types of rock phosphate (RP) were studied with respect to different parameters like temperature, incubation period, pulp density and effect of different carbon and nitrogen sources. Results indicated that the lower the concentration of the phosphate in the leaching broth the greater was the dissolution percentage of P2O5. A maximum of 44.70, 48.84 and 56.17(mg%) of P2O5 solubilisation were obtained after 15 days of incubation at 35ēC from West valley, North valley and South valley rock phosphate, respectively at 0.5 % pulp density. Acidic pH medium was favourable for phosphate solubilisation in all the experiments. Among the carbon sources glucose followed by maltose and sucrose supported the maximum RP solubilization in the presence of 0.5% pulp density as the optimum concentration. Nitrogen in the form of ammonium was very effective in solubilizing rock phosphates by P. fluorescence.

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