International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Isolation and Characterization of Multi drug Resistant Super Pathogens from soil Samples Collected from Hospitals

Author Affiliations

  • 1Dept. of Chemistry, D.A.V College, Kanpur, INDIA
  • 2 R and D Division, MRD Life Sciences, Lucknow, INDIA
  • 3 Integral University, Lucknow, INDIA
  • 4 Dept of Chemistry, V.S.S.D College, Kanpur, INDIA
  • 5 Dept of Chemistry, Christ Church College, Kanpur, INDIA

Res. J. Recent Sci., Volume 2, Issue (ISC-2012), Pages 124-129, February,2 (2013)


Soil samples from two different city hospitals were collected, pre-treated along with several antibiotics for primary screening of numerous microbes and were cultured after serial dilution over sterile nutrient agar plates. A total of three isolated were identified and purified from the samples, further screened for individual antibiotics at their respective varying concentrations and all the three isolates were found to be strong resistant against antibiotics selected in the study. Morphological, biochemical and physiological properties were analysed for all the isolates.


  1. Adegoke, Anthony A., Tom Mvuyo, Okoh Anthony and Jacob Steve Studies on multiple antibiotic resistant bacterial isolated from surgical site infection, Scientific Research and Essays, 5(24), 3876-3881 (2010)
  2. Dharmadhikari S.M. and Peshwe S.A. Molecular level studies on multiple and serum resistant in UTI pathogen, Indian Journal of biotechnology, 8, 40-45 (2009)
  3. Abbasi A.M., Mosaffa N.D.M., Taheri S. and Jaafari M.K., Effect of Anti Bacterial Skin Secretion of ―Rana ridibanda‖ Frog on Methycillin Resistant S. Aureus, Yakhteh Medical Journal, 9, 146–155 (2007)
  4. Rodrigues O.D., Cezrio C.R. and Filho G.P.P., Ventilator-Associated Pneumonia (VAP) caused by Multidrug-Resistant (MDR) Pseudomonas aeruginosa vs. other microorganisms at an adult clinical-surgical intensive care unit in a Brazilian University Hospital: Risk factors and outcomes, International Journal of Medicine and Medical Sciences, 1(10) 432-437(2009)
  5. Subha A., Devi R.V. and Ananthan S., AmpC b-lactamase producing multidrug resistant strains of Klebsiella spp. and Escherichia coli isolated from children under five in Chennai, Indian J Med Res., 177, 13-18 (2003)
  6. Oliveira A.C., Maluta R.P., Stella A.E., Rigobelo E.C., Jos Moacir Marin J.M. and Fernando Antonio de vila. Isolation of Pseudomonas aeruginosa strains from dental office environments and units in barretos, state of so paulo, brazil, and analysis of their susceptibility to antimicrobial drugs, Brazilian Journal of Microbiology, 39, 579-584 (2008)
  7. Kerry M., Jeanne F., Sue H., Cindy H., Nnielle H., Mary M. and Deborah A.B., An Emerging Multidrug- Resistant Pathogen in Critical Care 28(2008)
  8. Aibinu I.E., Peters R.F., Amisu K.O., Adesida S.A., Ojo M.O. and Tolu O., Multidrug Resistance in E. coli 0157 Strains and the Public Health Implication, J. American Sci., 3(3), 15-28 (2007)
  9. Bhatta D.R. and Kapadnis B.P., Production optimization and characterization of bioactive compound against Salmonella from Bacillus subtilis KBB isolated from Nepal, Scientific World, 8(8) (2010)
  10. Welch J.T., Fricke F.W., Patrick F., McDermott F.P., White G.D., Rosso L.M., Rasko D.A., Mammel K.M., Eppinger M., Rosovitz M.J., Wagner D., Rahalison, LeClerc E.J., Hinshaw M., Lindler E.L., Cebula A.T. Carniel and E. Ravel J. Multiple Antimicrobial Resistance in Plague: An Emerging Public Health Risk, PLoS ONE, 2(3), 309 (2007)
  11. Gales A.C., Jones R.N., Turnidge J., Rennie R. and Ramphal R., Characterization of Pseudomonas aeruginosa Isolates: Occurrence Rates, Antimicrobial Susceptibility Patterns and Molecular Typing in the Global SENTRY Antimicrobial Surveillance Program, 1997–1999, Clinical Infectious Diseases, 32(2),146–155(2001) , 124-129 (2013)
  12. Chiu H.L., Cheng-Hsun Chiu H.C., Horn M.Y., Chiou S.C., Lee Y.C., Yeh M.C., Chang-YouYu Y.C., Chao-Chin Chang C.C. and Chu C., Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates, BMC Microbiology, 10, 86 (2010)
  13. Mishra R.P., Singh Deepak S.P. and Anwar R., Study of culture conditions and antimicrobial drug production properties of Acinetobacter baumannii, J. Pharm. Biol. Sci., 12(16), 1-4 (2011)
  14. Davis R.D., McAlpine B.J., Pazoles J.C., Talbot K.M., Alder A.E., White C.A., Jonas M.B., Murray E.B., Weinstock M.G. and Rogers L.B., Enterococcus faecalis Multi-Drug Resistance Transporters: Application for Antibiotic Discovery, J. Mol. Microbiol. Biotechnol, 3(2) 179-184(2001)
  15. Hassan A., Usman J., Kaleem F., Khan A. and Hussain Z., In vitro activity of aminoglycosides, β lactam-β lactamases inhibitor combinations and tetracyclines against multi-drug resistant Acinetobacter baumannii, isolated from a tertiary care hospital, Journal of Microbiology and Antimicrobials, 2(4) (2010)
  16. Okonko I.O., Nkang A.O., Eyarefe O.D., Abubakar M.J., Ojezele M.O. and Amusan T.A., Incidence of Multi-Drug Resistant (MDR) Organisms in Some Poultry Feeds Sold in Calabar Metropolis, Nigeria, British Journal of Pharmacology and Toxicology, 1(1),15-28 (2010)
  17. Michalopoulos A. and Falagas M.E., Colistin and Polymyxin B in Critical Care, Crit Care Clin 24, 377–391 (2008)
  18. Megha Y.J., Alagawadi R.A. and Krishnaraj P.U., Multiple Beneficial Functions of Fluorescent Pseudomonads of Western Ghats of Uttara Kannada District, Karnataka J. Agric. Sci., 20(2), 305-309 (2006)
  19. Nino K., Juan J.Z., Ping C., Sitara N.W., Marian C.L., Charles J.G., Timothy J.T. and Patrick C.R., Loss of p53 Function Confers High-Level Multidrug Resistance in Neuroblastoma Cell Lines, Cancer Research, 61, 6185–6193 (2001)
  20. Rasool A.S., Ahmad A., Khan S. and Wahab A., Plasmid Borne Antibiotic Resistance Factors Among Indigenous Klebsiella. Pak. J. Bot., 35(2), 243-248 (2003)
  21. Stephen C.R., Joshua A.S., Megan M. and Milton C.W., Cost-Effectiveness of Treating Multidrug-Resistant Tuberculosis journal. Pmed, 10,1371 (2006)
  22. Struelens D.L., Monnet A.P., Magiorakos F., O’Connor S. and Giesecke J. New Delhi metallo-beta-lactamase 1–producing Enterobacteriaceae: emergence and response in Europe, 8(29) (2010)