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Genetic variability of Macrophomina phaseolina Affecting Sesame: phenotypic traits, RAPD markers and interaction with the Crop

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Author Affiliations

  • 1Departamento de Ciencias Biológicas, Decanato de Agronomía, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, VENEZUELA

Res. J. Recent Sci., Volume 2, Issue (ISC-2012), Pages 110-115, February,2 (2013)

Abstract

Macrophomina phaseolina is a fungus which affects more than 300 cultivated species. It is one of the most important biotic stresses on sesame (Sesamum indicum L.). A successful control strategy, especially plant resistance management, depends on comprehensive knowledge about genetic variability for both fungus and plant. To evaluate genetic diversity of M. phaseolina affecting sesame in the most important crop production region of Venezuela, seven isolates were characterized by means of phenotypic traits, and RAPD markers. Four of these isolates were used for evaluating the interaction with four sesame genotypes in two ways: interaction in vivo by inoculation, and effect of root and stem extracts on fungus growth. Variability for growth velocity (optical density ranged between 1.69 to 2.32 at 96 hours of growing) (P≤0.05) and microsclerotia production (18-56 in 100 μL) (P≤0.05), was observed. Ten primers used were able to amplify the DNA, generating 81 bands (100% polymorphic). Ordination of the seven isolates by means of principal coordinates analysis based on RAPD did not show a consistent relationship with phenotypic attributes or geographical origin. After inoculation, length lesion produced by the four isolates did not show statistical differences, but germination percentage did (P≤0.01). One of the fungus isolates reduced up to almost 70% average germination of the four sesame genotypes. Mycelial growth of four isolates was inhibited in 17-32 % as compared to the control. These results indicate it is difficult to manage charcoal rot by means of obtaining resistant cultivars because of the fungus variability found in all the levels evaluated..

References

  1. Wyllie T. Charcoal rot, In: Compendium of soybean diseases. Third edition. J.B. Sinclair and P.A. Backman (eds). Pages 30-33. American Phytopathological Society, St. Paul, MN, USA, (1989)
  2. Pineda J. Enfermedades del ajonjolí: algunas medidas de control. II Curso sobre producción de ajonjolí, soya y otras leguminosas, 114 pp. ASOPORTUGUESA, UCLA and FONAIAP, Venezuela (2002)
  3. Odvody G. and Dunkle L., Charcoal stalk rot of sorghum: effect of environment in host-parasite relations, Phytopathology, 126, 343-352 (1979)
  4. Pineda J. and Avila J., Alternativas para el control de Macrophomina phaseolina y Fusarium oxysporum patógenos del ajonjolí (Sesamum indicum L.), Agronomía Tropical, 38, 79-84 (1988)
  5. Pineda J., Nass H. and Rodríguez H. Efecto de la densidad de inóculo de (Macrophomina phaseolina) en la infección de plántulas de ajonjolí, Agronomia Tropica, 35, 133-138 (1985)
  6. Weiland J. and Sundsbak J. Differenciation and detection of sugar beet fungal pathogens using PCR amplification of actin coding sequences and the ITS Region of the Rrna. Gene, 84, 475-482 (2000)
  7. Laurentin H. Genética Agrícola. Editorial Académica Española. Saarbrücken, Germany (2011) , 110-115 (2013)
  8. Karp A., Kresovich S., Bhat K., Ayady W. and Hodgkin T., Molecular tools in plant genetic resources conservation: a guide to the technology, IPGRI Technical Bulletin No. 2. International Plant Genetic Resources Institute, Rome, Italy (1997)
  9. El-Bramawy M. Field resistance of crosses of sesame (Sesamum indicum L.) to charcoal root rot caused by Macrophomina phaseolina (Tassi) Goid, Plant Protection Science, 42, 66-72 (2006)
  10. Simosa N. and Delgado M. Virulence of four isolates of Macrophomina phaseolina on four sesame (Sesamum indicum) cultivars, Fitopatología Venezolana, 4, 20-23 (1991)
  11. De Lucca A., Cleveland T. and Wedge D. Plant-derived antifungal proteins and peptides, Canadian Journal of Microbiology, 51, 1001-1014 (2005)
  12. Khan S., Ayub N. and Ahmad I., Inhibitory effect of extracts of plant parts of sunflower hybrids on sclerotia production of Macrophomina phaseolina, Pak. J. Phytopathol, 19, 150-154 (2007)
  13. Csöndes I., Cseh A and Taller J. Genetic diversity and effect of temperature and pH on the growth of Macrophomina phaseolina isolates from sunflower fields in Hungary, Molecular Biology Reporter, DOI 10.1007/s11033-011-1094-6 (2011)
  14. Dellaporta S., Wood J. and Hicks J., A plant DNA minipreparation: versión II, Plant Mol. Biol. Rep., 1, 19-21 (1983)
  15. Beas-Fernández R., De Santiago A., Hernández-Delgado S. and Mayec-Pérez N., Characterization of Mexican and non-Mexican isolates of Macrophomina phaseolina based on morphological characteristics, pathogenicity on bean seeds and endoglucanase genes, Journal of Plant Pathology, 88, 53-60 (2006)
  16. Mahdizadeh V., Safaie N. and Goltapeh E., Diversity of Macrophomina phaseolina based on morphological and genotypic characteristics in Iran, Plant Pathology Journal, 27, 128-137 (2011)
  17. Almeida A., Abdelnoor R., Arrabal C., Carvalho V., Jacoud D., Marin L., Benato M. and Carvalho C. Genotypic diversity among Brazilian isolates of Macrophomina phaseolina revealed by RAPD, Fitopatología Brasileira, 28, 279-285 (2003)
  18. Jana T., Sharma T., Prasad R. and Arora D., Molecular characterization of Macrophomina phaseolina and Fusarium species by a single primer RAPD technique, Microbiological Research, 158, 249-257 (2003)