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Studies on Isolation and Characterization of Lactase Produced from Soil Bacteria

Author Affiliations

  • 1School of Community Science and Technology, Bengal Engineering and Science University, Shibpur-711 103, Howrah, West Bengal, INDIA

Res. J. Recent Sci., Volume 2, Issue (8), Pages 92-94, August,2 (2013)


The enzyme β-galactosidase has many applications in biotechnology, pharmaceuticals and food processing industries. The present study was conducted to screen the soils from cattle shed near Kolkata for the isolation of lactase producing bacteria. The isolation was performed by plating on lactose agar medium containing the chromogenic substance X-gal. o-nitrophenyl-β-D-galactopyranoside was used as a substrate to determine the β-galactosidase activity of the two isolates BS1 and BS2 which were found to be the active strain producing a considerable amount of β-galactosidase. The bacterial isolate BS1 showed maximum β-galactosidase activity under shaking condition. Both BSI and BS2 produced extracellular and intracellular β-galactosidase activity. The optimum pH and temperature for enzyme activity were 7 and 40°C respectively.


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