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Estimation of Levetiracetam in Bulk and Pharmaceutical Dosage Form with a Newly Developed and Validated RP-HPLC Method

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Author Affiliations

  • 1Mohi Ud Din Islamic Institute of Pharmaceutical sciences, Mirpur, Azad Jammu and Kashmir, PAKISTAN
  • 2 Department of Chemistry, Gomal University, Dera Ismail Khan, PAKISTAN
  • 3 PIMS, Islamabad, PAKISTAN
  • 4 Faculty of Pharmacy; Hamdard University, Karachi, PAKISTAN

Res. J. Recent Sci., Volume 2, Issue (5), Pages 68-70, May,2 (2013)

Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Levetiracetam in bulk and tablets dosage forms. The separation was achieved on C18 analytical column (250 mm × 4.6 mm i.d., 5.0 µm) using acetonitrile and distilled water in the ratio 80:20 v/v as mobile phase and at a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 210nm. The total chromatographic analysis time per sample was about 5.0min with Levetiracetam eluting at retention time of about 3.5min. The method is accurate (99.51% - 100.45%), and the standard curve was linear over the concentration range of 25-150µg/mL with R2 close to one (0.999) and Y-intercept of 0.022. The limit of detection (LOD) and limit of Quantitation (LOQ) obtained for Levetiracetam were 0.01µg/mL and 0.09µg/mL, respectively. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the determination of Levetiracetam in tablets dosage form.

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