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Estimation of Levetiracetam in Bulk and Pharmaceutical Dosage Form with a Newly Developed and Validated RP-HPLC Method

Author Affiliations

  • 1Mohi Ud Din Islamic Institute of Pharmaceutical sciences, Mirpur, Azad Jammu and Kashmir, PAKISTAN
  • 2 Department of Chemistry, Gomal University, Dera Ismail Khan, PAKISTAN
  • 3 PIMS, Islamabad, PAKISTAN
  • 4 Faculty of Pharmacy; Hamdard University, Karachi, PAKISTAN

Res. J. Recent Sci., Volume 2, Issue (5), Pages 68-70, May,2 (2013)

Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Levetiracetam in bulk and tablets dosage forms. The separation was achieved on C18 analytical column (250 mm 4.6 mm i.d., 5.0 m) using acetonitrile and distilled water in the ratio 80:20 v/v as mobile phase and at a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 210nm. The total chromatographic analysis time per sample was about 5.0min with Levetiracetam eluting at retention time of about 3.5min. The method is accurate (99.51% - 100.45%), and the standard curve was linear over the concentration range of 25-150g/mL with R2 close to one (0.999) and Y-intercept of 0.022. The limit of detection (LOD) and limit of Quantitation (LOQ) obtained for Levetiracetam were 0.01g/mL and 0.09g/mL, respectively. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the determination of Levetiracetam in tablets dosage form.

References

  1. Tripathi K.D., Essential of Medical Pharmacology, 6th ed., 410 (2010)
  2. The Merck Index, 13th Edn., Merck and Co., Inc., Whitehouse Station, NJ, 978 (2001)
  3. Farooq M.U., Bhatt A., Majid A., Gupta R., Khasnis A., Kassab M.Y., Levetiracetam for managing neurologic and psychiatric disorders, Am J Health Syst Pharm, (66)6, 54161 (2009)
  4. ICH, Q2 (A), Validation of analytical procedures: text and methodology International Conference on Harmonization. Geneva, 1- 13 (2005)
  5. International Conference on Harmonization, Guideline on Validation of Analytical Procedure-Methodology, Geneva, Switzerland (1996)