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Genetic and Phytochemical analysis of Cluster bean (Cyamopsis tetragonaloba (L.) Taub) by RAPD and HPLC

Author Affiliations

  • 1Biochemistry (ICAR), Vivekanand Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, INDIA
  • 2 Department of Bioscience & Biotechnology, Banasthali University, Banasthali-304022, Rajasthan, INDIA

Res. J. Recent Sci., Volume 2, Issue (2), Pages 1- 9, February,2 (2013)

Abstract

In recent years chemoprofiling and molecular phylogenetic studies have received considerable attention and used simultaneously in higher plants to characterise the plant species. Guar or cluster bean, (Cyamopsis tetragonoloba (L.) Taub) is a drought-tolerant annual legume crop. India is the world-leader for cluster bean production as it contributes 80% shares of its total production. The aim of the present work is to determine and evaluate the degree of polymorphism in cultivars grown in Rajasthan using RAPDs and to explore the correlation among RAPD and chemical markers in five varieties of guar RGC-936, 1002, 1003, 1031 and 1017 were taken. Phenolic acids such as sinapic acid, cholorogenic acid, caffeic acid and gallic acids were detected among all cultivars. Whereas flavanoids i.e. kaempferol and myricetin were showing variations among all cultivars. Polyphenols are powerful antioxidant and flavonoids play role in the prevention of degenerative diseases such as cancer and cardiovascular diseases is emerging. The phytochemical analysis of guar may expand its nutraceutical and pharmaceutical utilization and information from this study will be useful to breeding programmes for improving guar seed quality.

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