6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Enhancement of Secondary Metabolite Biosynthesis in Bacopa monnieri: An in vitro Study

Author Affiliations

  • 1 Life Science Department, Maharaja Ranjit Singh College of Professional Sciences, Indore-17, MP, INDIA

Res. J. Recent Sci., Volume 2, Issue (1), Pages 13-16, January,2 (2013)

Abstract

Enhanced production of total saponins was obtained from 40 day old suspension cultures of Bacopa monnieri. The callus yielded not only a 166% increased concentration of saponins but also produced two novel prominent bands of saponins compared to the natural plant system. We report here for the first time an increased potential of cell lines of B. monnieri in suspension for production of saponins; the active constituents responsible for neuropharmacological and nootropic action of the plant. Our studies can be utilised in meeting the demand and supply ratio of the drug with constant and elevated production of saponins produced in short duration.

References

  1. Gohil K.J. and Patel J.A., A review on B. monniera; Current research and future prospects, International Journal of Green Pharmacy, 4(1), 1-9 (2010)
  2. Tiwari V., Tiwari K.N. and Singh B.D., Suitability of liquid cultures for in vitro multiplication of Bacopa monniera (L.) Wettst., Phytomorph., 50, 337-342 (2000)
  3. Garay S., Mahato S.B., Ohtani K., Yamasaki K., Dammarane type triterpenoid saponins from Bacopa monnieri, Phytochem., 42, 815-820 (1996)
  4. Murthy P.B.S., Raju V.R., Ramakrisana T., Chakravarthy M.S., Kumar K.V., Kannababu S. and Subbaraju G.V., Estimation of twelve Bacopa saponins in Bacopa monnieri extracts and formulations by high performance liquid chromatography, Chem. Pharm. Bull.,54(6), 907-911 (2006)
  5. Sajc L., Grubisic D. and Vunjak-Novakovic G., Bioreactors for plant engineering: An outlook for further research, Biochem Eng ., 4, 89-99 (2000)
  6. Kieren P.M., Mac Loughlin P.F. and Malone D.M., Plant cell suspension cultures: some engineering considerations. J. Biotechnol., 5939-52 (1997)
  7. Jain M., Tiwari S., Guruprasad K.N. and Pandey G.P., Influence of different media on somatic embryogenesis of Bacopa monnieri. J. Trop. Med. Plants, 11(2), 163-167 (2010)
  8. Rahman L.U., Verma P.C., Singh D., Gupta M.M., and Banerjee S., Bacoside production by suspension cultures of Bacopa monnieri (L.) Pennell, Biotechnology Letters24, 1427-1429, (2002)
  9. Ebrahimzadeh H and Niknam V., A revised spectrophotometric method for determination of triterpenoid saponins, Indian Drugs35 (6), 379-381 (1998)
  10. Vijaya Sree N., Udayasri P.V.V., Aswani kumar Y., Ravi Babu B., Phani kumar Y., Vijay Varma M., Advancements in the Production of Secondary Metabolites, Journal of Natural Products, , 112-123 (2010)
  11. Oksman-Caldentey K.M., Inze D., Plant cell factories in the post genomic era: new ways to produce designer secondary metabolites, Trends Plant Sci., , 433-440 (2004)
  12. Rao R.S., Biotechnological production of phytopharmaceuticals,Journal of Biochemistry Molecular Biology and Biophysics, 4,73112 (2000)
  13. Mathur A. and Mathur A.K., Medicinal Plant Biotechnology, CAB International, 115-137 (2010)
  14. Mousumi D., Malik C.P., Bisen P.S., Micropropagation: A tool for the production of high quality plant-based medicines, Curr. Pharm. Biotechnol., 33-49 (2006)
  15. Zhang Q., Rich J.O., Cotterill I.C., Pantaleone D.P., Michels P.C., 14-Hydroxylation of opiates: Catalytic direct autoxidation of codeinone to 14-hydroxycodeinone, J. Am. Chem. Soc., 127, 7286-7287 (2005)