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In vitro enzyme inhibitory and free radical scavenging potentials of an Aegle marmelos endophytic actinomycete extract

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Author Affiliations

  • 1Department of Microbiology, Punjab Agricultural University, Ludhiana, India
  • 2Department of Microbiology, Punjab Agricultural University, Ludhiana, India

Res. J. Recent Sci., Volume 11, Issue (1), Pages 24-27, January,2 (2022)

Abstract

Actinomycetes are potential sources to discover new biologically active metabolites. While Aegle marmelos has been widely studied for the bioactive potentials of its endophytic fungi and bacteria, the endophytic actinomycetes from this plant have been far lesser exploited. An endophytic actinomycete obtained from Aegle marmelos was tested for its in vitro enzyme inhibitory capabilities. The supernatant was extracted in ethyl acetate to prepare various concentrations (100-1000µg/ml). The extract was found to exhibit 50% inhibition (IC50) of alpha-amylase and alpha-glucosidase, at concentrations of 1950.71 ± 0.11µg/ml and 391.38± 0.09µg/ml, respectively. Ethyl acetate extracts were also tested for their reducing activity and free radical scavenging (hydroxyl radical, superoxide anion, nitric oxide free radical) abilities. Total phenol contents were determined to be 42.11±1.88mg/g of the extract (catechol equivalents) and 6.47±0.95mg/g of the extract (gallic acid equivalents). New acquisitions in these fields will be fundamental in order to exploit actinomycetal strains from Aegle marmelos, which can exhibit bioactive properties.

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