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Histochemical analysis of the digestive tract of larvae of Potato tuber moth (Phthorimaea operculella zell) on the basis of their food habits

Author Affiliations

  • 1Dr. B.R. Ambedkar University, Agra, India

Res. J. Recent Sci., Volume 11, Issue (1), Pages 20-23, January,2 (2022)

Abstract

In this study the potato tuber moth (Phthorimaea operculella zell), one of the most common pest of potato crops (solanaceous sp.) was investigated to determine the impact of food habit on the histochemical analysis of the digestive tract of its larval form. Histochemical observations were made on amino acids (tyrosine and tryptophane), lipids (neutral lipids and phospholipids), enzymes (alkaline phosphatase and acid phosphatase) and basic proteins (fibrin and reticulin). These histochemical observations reveal a very significant correlation to the food habit of the moth.

References

  1. Bansley, R.R and Gersh, I. (1933)., Studies on all structure by freezing drying method, nature of mitochondria in hepatic cell of Amblyostoma., Anat. Rec., 57-217.
  2. Adams, A. (1957)., A P-dymethylaminobezaldehyde nitrite method for the histochemical demonstration of Tryptophane and related compounds., J. Clin. Pathol., 10, 56-62.
  3. McManus, J. F. A. (1946)., The demonstration of certain fatty substances in paraffin sections., The Journal of pathology and bacteriology, 58(1), 93-95.
  4. Backer J.R. (1946)., The histochemical recognition of lipid., Quart. J. Micr. Sci., 97, 441-443
  5. Gomori, G (1952)., Microscopic histochemistry principles practice., The university of Chicago press. Chicago Illinois p. 312-314.
  6. Mallory, F B (1938)., Pathological techniques., Saunders, Philadelphia.
  7. Gorden, H & Sweets, H H Jr. (1967)., In Carleton’s hostological technique., Oxford University, Press Toronto (1967)
  8. Gautam, R.K. & Masih, S. (2002)., Intestinal amino acids alters due to varied food habits in moth larvae., J. Natcon. 14(2), 269-272.
  9. Gautam R.K. & Sharma S.B. (1993)., Histochemical and Biochemical studies of digestive tract of three Acridid Grashoppers (Orthoptera: Acrididae) with different food habits., Ph.D. Thesis, Agra University, Agra.
  10. Hiromu, A. (1969)., Ultrastructure localization of phosphatases in midgut of silkworm, Bombyxmori., J. Insect Physiol, 15, 1623.
  11. Furquhar, M.G., J.J.M. Bergersson & G. E. Palade (1974)., Cytochemistry of golgi fractions prepared from rat liver., J. Cell. Biol., 60, 8.
  12. Thorsteinson, A J (1960)., Host Selection in phytophagus insects., Annu. Rev. Entromol., 5, 193-218.