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Evaluation of one Rapid Method for Diagnosis of Malaria The Optional and Better Replacement of Microscopy

Author Affiliations

  • 1Dolat Usha Institute of Applied Sciences, Valsad; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA
  • 2Shree Ram Krishna Institute of Comp. Edu. and Applied Sciences; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011), Pages 361-364,(2012)


Microscopy has been the most trustable technique for the diagnosis of malaria in India. Reduction of morbidity and mortality rate of malaria highly influenced by earlier and proper diagnosis. This study was carried out at Valsad, Gujarat. It involved use of microscopy i.e. field’s stain and detection of Plasmodium falciparum - HRP II antigen, Plasmodium vivax - pLDH antigen detection by one rapid diagnostic Test (RDT) SD Bioline. Present study was carried out from 966 EDTA anticoagulated samples collected from clinical laboratories and hospitals of Valsad. Microscopic examinations of stained thick and thin films, shows 8.39%, 13.97%, 0.21% were detected as Plasmodium falciparum, P. vivax and mix respectively. Whereas with Rapid Diagnostic test using HRP II, p-LDH antigens 9.05% and 13.87% were detected as P. falciparum, P. vivax respectively. The study shows reasonable harmony between microscopy and RDT. Among two methods RDT was found to have high sensitivity (97.70%) and specificity (98.93%) compared to microscopy. Though the microscopic method is cost effective but laborious and needs an expertise. The RDT results were highly accurate and can be used where microscopy is inaccurate or in case of unavailability of expert.


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