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Biodiversity of Actinomycetes in Hypersaline soils of Kolhapur district and their screening as potential antibiotic producer, India

Author Affiliations

  • 1Dept. of microbiology, govt. Rajaram college,vidyanagar, Kolhapur, MS, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011),

Abstract

Hyper saline Soil samples from different sites in Kolhapur district of Maharashtra were analysed over a period of one year for actinomycetes population. Microscopic, cultural, biochemical characters, pigmentation, cell wall analysis and spore morphology and other criteria were used to identify the Actinomycetes isolates. Members of genus Streptomyces were identified by MICRO-IS software1. Isolates other than Streptomyces genus were identified using Bergeys manual of systematic bacteriologyy2. The actinomycetes species identified are Streptomyces (37),Streptoverticillum (20),Micromonospora (15),Nocardia (13),Microbispora (08),Actinoplanes (5),Planomonospora(04),Kitasatosporia (3).Isolates were screened for antibacterial and antifungal activity by Agar overlay technique using two bacterial and four fungal species.Results indicated that the hyper saline soils are rich in biodiversity of actinomycetes, 4 species showed antifungal activity and 10 showed antibacterial activity.Present study indicated that the halophillic actinomycetes have a great potential to be an antibiotic producer and can be explored for antibiotic production.

References

  1. Richards L.A., Diagnosis and improvement of Salinealkali soil, USDA Handbook No.60, Wasington, 69-82(1954)
  2. Kushner D.J., the Halobacteriaceae. In the Bacteria, atreatise on structure and function, C.R. Woese and R.S.Wolfe, Academic press, 8, 171-214 (1985)
  3. Rodriguez Valera F., Characteristics and Microbialecology of Hyper saline environment, In HalophilicBacteria, (ed. F. Rodriguez-Valera, CRC press). BocaRaton (Florida), III, 30, (1986)
  4. Smit F.B., An investigation on a taint in ribbons ofbacon, the determination of halophillic Vibrios, (n.sp.)Proc.R.Coc.Queensland,49, 29-53 (1938)
  5. Quesda E.A., Ventosa F., Rodriguez Valera And A.Ramos-cormenza, Types and properties of some bacteriaisolated from hypersaline soils, J.Appl.Bacteriol, 53,155-161 (1982)
  6. Quesda E.A., Ventosa F., Rodriguez-valera , Megias I.and Ramos-cormenza A., Numerical taxonomy ofmoderately halophilic bacteria isolated from hypersalinesoils, J.Gen. Microbio, 129, 2649-57, (1983)
  7. Quesda E., Valdermma M., Bejar V., VentosaA., Rulzberra quero F. And Ramos ormenza, A. Syst.Appl.Microbiol, 9,132-137, (1987)
  8. Collins C.H., Lyne P.M. and Granje J.M., Microbiological methods, Butterworth and Heinemannpub, (1995)
  9. Becker B., Lechevalier M.P. and Lechevalier H.A., Chemical composition of cell wall preparation fromstrains of various form-genera of aerobic actinomycetes, Appl.Microbiol, 13,236-243, (1965)
  10. Staneck J.A. and Roberts G.D., Simplified approach toidentification of aerobic actinomycetes by thin layerchromatography, J.Appl.Microbiol, 28, 226-23, (1974)
  11. Gibbs B.M. and Shapten D.A., Identification methodsfor microbiologists part, B.Academic press, New York,(1968)
  12. Portyrata D.A. and Krichevsky M.I., MICRO-IS, Amicrobiological database management and analysissystem, Binary, 4, 31-36 (1992)
  13. Williams S.T.,Sharpe M.E. and Holt J.G., Bergeysmanual of systematic bacteriology, Vol. 4,The Williamsand Wilkins co.Baltimore (1989)Erikson D., studies onsome lake mud strains of Micromonospora, J. Bacteriol,41, 227-300 (1941)
  14. Jiang C.L. and Xu L.H., Diversity of acquaticactinomycetes in lakes of middle plateau,Yunan, China,Appl.Env.Microbiol, 62, 249-253 (1996)
  15. Silvey J.K.G. and Roach A.W., The taste and odourproducing actinomycetes, Crit.Rev.En.control, 5, 233-273, (1975)
  16. Cross T. and Al-Diwany L.J., Streptomycetes withsubstrate mycelia spores, the genus Elytrosporangium.Zentrablatt ,Fur baktriologie, Microbiologie andHypiene, 1, Abteilung, Supplement, 11, 59-65 (1981)
  17. Mickovski M., Microflora of saline soils in Republic ofMacedonia Ann, Fac. Agri. Skopje, 14, 277-298 (1961)