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Biodiversity of Actinomycetes in Hypersaline soils of Kolhapur district and their screening as potential antibiotic producer, India

Author Affiliations

  • 1Dept. of microbiology, govt. Rajaram college,vidyanagar, Kolhapur, MS, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011),


Hyper saline Soil samples from different sites in Kolhapur district of Maharashtra were analysed over a period of one year for actinomycetes population. Microscopic, cultural, biochemical characters, pigmentation, cell wall analysis and spore morphology and other criteria were used to identify the Actinomycetes isolates. Members of genus Streptomyces were identified by MICRO-IS software1. Isolates other than Streptomyces genus were identified using Bergeys manual of systematic bacteriologyy2. The actinomycetes species identified are Streptomyces (37),Streptoverticillum (20),Micromonospora (15),Nocardia (13),Microbispora (08),Actinoplanes (5),Planomonospora(04),Kitasatosporia (3).Isolates were screened for antibacterial and antifungal activity by Agar overlay technique using two bacterial and four fungal species.Results indicated that the hyper saline soils are rich in biodiversity of actinomycetes, 4 species showed antifungal activity and 10 showed antibacterial activity.Present study indicated that the halophillic actinomycetes have a great potential to be an antibiotic producer and can be explored for antibiotic production.


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