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Levels of Glutathione S - Transferase In Different Larval Tissues of Papilio Demoleus

Author Affiliations

  • 1Department of Zoology, Shankarlal Khandelwal College, Akola, M.S., INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011), Pages 313-316, (2012)

Abstract

Papilio species are of economic importance, as their larvae are important defoliator of citrus known from almost all the citrus growing areas of the world. As a result, the overall vigour and vitality of the citrus plant is reduced considerably. The aim of the present article is to study the glutathione S-transferase in different larval tissues of Papilio demoleus. CDNB - conjugation activity was compared in various fifth instar larval tissues of laboratory reared and field collected Papilio demoleus using CDNB (1-chloro-2, 4-dinitrobenzene) as substrate. Different tissues estimated for GST include the midgut, fat bodies, cuticle, haemolymph and the whole body of two days old fifth instar larvae. The GST activity was found highest (648.93 ± 0.08 ΅M mg protein-1min-1) in fat bodies of field-collected larvae while the corresponding value in the laboratory-reared larvae was 80.19 ± 0.05 ΅M mg protein-1min-1, where it showed 8.09 fold increases in GST activity. The fat bodies showed highest GST activity suggesting that the fat body is the major site of metabolism of insecticide, especially the organophosphate class, whiles the gut, cuticle and haemolymph also plays an important role in the metabolism of insecticide.

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