International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Larvicidal Potential of Biologically Synthesised Silver Nanoparticles against Aedes Albopictus

Author Affiliations

  • 1 School of Environmental Studies, Cochin University of Science and Technology, Kochi, Kerala, INDIA
  • 2 Rajiv Gandhi Chair in Contemporary Studies, Cochin University of Science and Tech., Kochi, Kerala, INDIA
  • 3 Department of Chemical Oceanography, Cochin University of Science and Technology, Kochi, Kerala INDIA
  • 4 Soil Science Department, Kerala Forest Research Institute, Peechi, Thrissur, Kerala, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011), Pages 52-56, (2012)


Understanding of biological processes at the nanoscale level is a strong driving force behind development of nanotechnology. Silver nanoaparticles have important applications in the field of biology. Stable silver nanoparticles were synthesized by biological reduction method. The objective of the present study was to evaluate the larvicidal activity of silver nanoparticles synthesized from aqueous leaf extract of Hibiscus rosasinensis against the larvae of Aedes albopictus mosquito reared in rubber plantation. The parasite larvae were exposed to varying concentrations of aqueous extract of Hibiscus rosasinensis and synthesized silver nanoparticles for 24 h as per WHO protocols. Distilled water served as control. Percentage mortality was recorded. The synthesized nanoparticles exhibited significant larvicidal activity. This method is considered as an innovative alternative approach using green nanochemistry technique to control vector parasites and is the first report on mosquito larvicidal activity of Hibiscus rosasinensis leaf mediated synthesized silver nanoparticles.


  1. Sumodan P.K., Potential of rubber plantations as breeding source of Aedes albopictus in Kerala, India. The South East Asia and Western Pacific Region, WHO, Dengue bulletin, 27, 206-7 (2003)
  2. Liu N., Xu Q., Zhu F., Zhang L., Pyrethroid resistance in mosquitoes, Insect. Sci., 13, 159–166 (2006)
  3. Sareen Sarah John and John K.C., Lagenidium giganteum, an effective microbial control agent for Aedes mosquitoes in the rubber plantations, Proceedings of the 98th Indian Science Congress January 2011, Environmental Sciences, 93 (2010)
  4. Pillai Raji K., Singh Bishwajeet, Punj Deep., Kailasnath M., Chandramohanakumar N., Green synthesis and characterization of silver nanoparticles using the leaf and flower extract of Hibiscus rosasinensis, Proceedings of International Conference on Nanostructured Materials, France, 306 (2010)
  5. Lounibos L.P., Invasions by insect vectors of human disease, Annu Rev Entomol, 47, 233-266 (2002)
  6. Passos R.A., Marques Gram, Voltolini J.C. and Condino M.L.F., Dominance of Aedes aegypti over Aedes albopictus in the southeastern coast of Brazil, Rev Saude Publica, 37, 729-734 (2003)
  7. Juliano S.A., Lounibos L.P. and O’Meara G.F., A field test for competitive effects of Aedes albopictus on A. aegypti in South Florida, Differences between sites of coexistence and exclusion, Oecologia., 139, 583-593 (2004)
  8. Honorio N.A., Cabello P.H., Codeo C.T. and Lourenco-de-Oliveira R., Preliminary data on the performance of Aedes aegypti and Aedes albopictus immatures developing in water-filled tires in Rio de Janeiro, Mem Inst Oswaldo Cruz., 101, 225-228 (2006)
  9. Hawley W.A., The biology of Aedes albopictus, J Am Mosq Control Assoc., 4, 1-39 (1988)
  10. Philippe Parola, Xavier L., Jacus J., Clarisse R. and Remi N.C., Novel Chikungunya virus variant in travellers returning from Indian Ocean Islands, Emerging infectious diseases, 12(10), 1493-1498 (2006)
  11. Mashhadani A.L., Davidson H. M. and Curtis G.C., A genetic study of the susceptibility of Anopheles gambiae to Plasmodium bergei, Trans. Roy. Soc. of Tropical Med. and Hygiene, 749(5), 585 – 594 (1980)
  12. Anosike J.C. and Onwuliri C.O.E., Experiment on Wuchereria bancrofti infection of Culex quinquefasciatus and Aedes aegypti, Angew Parasitology, 33, 139 – 142 (1992)
  13. WHO - Report of the WHO informal consultation on the evaluation on the testing of insecticides CTD/WHO PES/IC/., 96.1, 69 (1996)
  14. Abbott W.S., A method of computing the effectiveness of an insecticide, Journal of Economic Entomol., 18, 265-267 (1925)
  15. Rai M., Yadav A. and Gade A., Silver nanoparticles as a new generation of antimicrobials, Biotechnol.Adv., 27, 76-83 (2009)