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Some growth studies on hyper EPS producing synechococcus spp. From different Industrial and coastal regions of Gujarat, India

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Author Affiliations

  • 1 Singhania University, Pacheri Bari, Jhunjhunu, Rajasthan, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011), Pages 33-40,(2012)

Abstract

Cyanobacteria are simplest form of algae, a representing of plant kingdom. They are widely distributed over land, water and often in environments where no other vegetation can exists. They've also distinction of being the oldest known fossils and account almost 8% of total population of bacteria in water bodies. Polysaccharides are renewable resources representing an important class of polymeric materials of biotechnological interest, offering a wide variety of potentially useful products to mankind. Extracellular polysaccharide of microbial origin have a novel functionality, reproducible physico-chemical properties, stable cost and supply, and so are better alternative to polysaccharides of plant and higher algal origin. Cyanobacteria are better suited than macro algae or higher plants, since they exhibit high growth rate and are more amenable to manipulation of conditions for enhancing growth or EPS production. Synechococcus spp. was isolated from different coastal and industrial regions of Gujarat and its growth kinetics were studied by using internationally recommended medium at various light intensities and production of the Exopolysaccharides was carried out under controlled growth conditions. Qualitative and quantitative estimations of total carbohydrates and proteins were carried out using Duboi’s method and Folin-lowrey’s method respectively. Total carbohydrates and proteins concentrations were measured at regular time interval of 5 days and gradual increase in concentration (Ranging from 539 µg/l to 588 µg/l ) of carbohydrates and (Ranging from 72 µg/l to 109 µg/l ) of proteins were found between 25 to 38 days. SDS-PAGE was carried out for further identification of proteins and two moieties of proteins were found having molecular weight of 65 kDa and 30 kDa respectively.

References

  1. De philippis R., Margheri M.C., Materassi R. and Vincenzini M., Potential of unicellular cyanobacteria from saline environments as exopolysaccharide producers. Appl. Environ. Microbiology, 64:1130-1332 (1998)
  2. Desikachary T. V., ‘Cyanophyta’, Indian council of agriculture Research, New Delhi (1989)
  3. Castenholtz R.W., Phylum BX. Cyanobacteria Oxygenic Photosynthetic Bacteria, In Bergey’s Manual of Systematic Bacteriology. Volume 1 The Archaea and the Deeply Branching and Phototropic Bacteria. Second Edition. G. Garrity, D. R. Boone, and R. W. Castenholtz (eds.) Springer-Verlag, New York (2001)
  4. Castenholtz, R. W. Species usage, concept, and evolution in the cyanobacteria (blue-green algae). Journal of Phycology 28,737-745 (1992)
  5. Allen M.M., Simple conditions for growth of unicellular blue green algae on plates. J. Phycol, 4,1-4 (1968)
  6. Allen M.M. and R.Y. Stanier, Selective isolation of blue-green algae from water and soil. J. Gen. Microbial, 51, 203-209, 26 (1968)
  7. Shah V., Garg, N., Madamwar, D., Exopolysaccharide production by a marine cyanobacterium cyanothece spp Application in dye removal by its gelation phenomenon, Applied Biochem. Biotechnology, 82, 81-90 (1999)
  8. B.D. Kaushik, 2011 Cyanobacterial Biotechnology, BTBT-(2011)
  9. Shah V., Ray, A. and Madamwar D., Characterization of the extracellular polysaccharide produced by a marine cyanobacterium, cyanothecea spp. ATCC 51142 and its exploitation toward metal removal from solutions. Current Microbial. 40: 274-278 (2000)
  10. Shah V., Garg N. and Madamwar D., ‘record of the cyanobacteria present in the Hamisar pond of Bhujm India’, Act. Bot. Mala, 25: 175-180 (2000)
  11. Stanier R.Y. and Cohen-Bazire G., Phototrophic Prokaryotes: The Cyanobacteria. Annual Review of Microbiology 31:225-274 (1977)
  12. A.P.H.A., Standard methods for the Examination of Water and Wastewater’s, American Public Health Association, New York, USA, 19th Edition
  13. Dubois M., Gilles K.A., Hamilton J.K., Rebers, P.A., smith F., Colorimetric method for determination of sugars and related substances, Anal. Chem. 28, 350-356 (1956)