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Juvenoid activity of some non mulberry plant extractives through inhibition of chitin deposition in the integument of fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2)

Author Affiliations

  • 1 Department of Zoology, Shardabai Pawar Mahila College, Shardanagar, Tal. Baramati, Dist Pune, INDIA
  • 2 Department of Zoology, Arts, Commerce and Science College, Indapur, Tal. Indapur Dist. Pune, INDIA

Res. J. Recent Sci., Volume 1, Issue (ISC-2011), Pages 1-6,(2012)

Abstract

Phytophagus insects derive nutrients and growth promoting biocompounds from the variable or specific flora available for them. The plants are the richest source of juvenile hormone analogues for phytophagus insects like silkworm, Bombyx mori (L). Fraction of plant derived insects juvenoids serve to take pause in the progression of metamorphosis through arresting some of the biochemical reactions including chitin synthesis or accelerating progression through other biochemical pathways in the larval body of insects. Ten microliters of each selected concentrations of acetone extractives of selected non mulberry flora (Vitis vinifera; Alstonia scholaris; Santalum album; Lantena camera; Syzyguim cumuni and Tectona grandis) were topically applied to individual larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) at 48 hours after the fourth moult Body wall chitin of untreated control larvae; acetone treated control larvae and treated larvae was estimated at 120 hours after the fourth moult. Topical application of selected concentrations of acetone extractives of selected non mulberry plants to fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) was found effected into the reduction in the deposition of chitin in the larval body wall. This reduction in body wall chitin was found ranging from zero to ninety eight percent. The plot of concentrations of acetone extractives of plant and percent reduction in the body wall chitin was found exhibiting a characteristic S- form of displacement, which herewith titled as Punyamayee Dose Response Curve. Since the effects of juvenoids involve the inhibition of metamorphosis through reduction in chitin deposition, it is possible to express the concentration (dose) applied in terms of ID50 value. The ID50 value of juvenoid contents of selected non mulberry flora can be defined as the specific unit (microgram), which enable to chitin to deposit fifty percent less in the body wall of larvae ( In comparison with untreated control). Accordingly ,the ID50 values calculated from the Punyamayee Dose Response Curves for non mulberry plants : Vitis vinifera; Alstonia scholaris; Santalum album; Lantena camera; Syzyguim cumuni and Tectona grandis were found measured 1.27; 1.40; 2.325; 2.86;3.60 and 4.04 micrograms respectively. The variation in the ID50 values among the non mulberry flora for the fifth instar larvae of silkworm, Bombyx mori (L) (Race : PM x CSR2) in the study may be concerned with quantity of acetone soluble juvenoid contents of the plants. Acetone soluble juvenoid content of non mulberry flora may be utilized efficiently for the fortified development of fifth instars of silkworm, Bombyx mori (L) and thereby, the cocoon quality. Sigmoid (S-form) Punyamayee Dose Response Curve may help for quantitative estimation of juvenoid contents of various plants.

References

  1. Zaoral M. and Slama K., Peptides with juvenile hormone activity, Science, 170, 92-93 (1970)
  2. Gopakumar, B., Ambika B. and Prabhu V.K.K., Juvenmimetic activity in some south Indian plants and their probable cause of this activity in Morus alba (L), Entomon, 2, 259 – 261 (1977)
  3. Slama K., Insect Hormones and anti-hormones in plants. Herbivores, their interaction with secondary plant metabolites (Eds. G.A. Rosenthal and D. H. Janzen; Academic Press, New York) 683-700 (1979)
  4. Ajami, A. M. and Riddiford, L.M., Comparative metabolism of the cecropia juvenile hormone, J. Insect Physiol., 19, 635 – 646 (1973)
  5. Grenier and Grenier, Fenoxycarb a fairlynew growth regulator: a review of its effects on insects, Ann. App. Biol., 122, 369–403 (1983)
  6. Khyade V.B., Patil S.B., Khyade S.V. and Bhawane G.P., Influence of acetone maceratives of Vitis vinifera on the larval parameters of silk worm, Bombyx mori (L), Indian Journal of Comparative Animal Physiology, 20, 14-18 (2002)
  7. Kamimura M. and Kiguchi M., Effect of juvenile hormone analogue on fifth stadium larvae of silk worm, Bombyx mori (L) (Lepidoptera: Bombycidae), Appl. Entomol. Zool. 33 (2), 333–338 (1980)
  8. Baishya, R.L. and Hazarika, L.R., Effect of methoprene and diflubenzuron on water, lipid, protein and chitin of Dicladispa armigera (Coleoptera, Chrysomelidae), Entomon, 21 91(1), 7-11, (1996)
  9. Calvez B., Hirn, M.and Reggi M., Progress of development programme during the last larval instar of Bombyx mori (L). Relationship with food intake, ecdyosteroids and juvenile hormone. Journal of Insect Physiology, 24 (4), 233–239, (1976)
  10. Jadhav G. and Kallapur V.L., Contribution of tissue protein to the cocoon shell in the fifth instar silk worm, Bombyx mori (L) (1989)
  11. Khyade V.B., Patil S.B., Khyade S.V. and Bhawane G.P., Influence of acetone maceratives of Vitis vinifera on the economic parameters of silk worm, Bombyx mori (L), Indian Journal of Comparative Animal Physiology, 21, 28–32, (2003)
  12. Khyade V.B., Influence of juvenoids on silk worm, Bombyx mori (L). Ph.D. Thesis, Shivaji University, Kolhapur, India, (2004)
  13. Ratnasen, How does juvenile hormone cause more silk yield, Indian Silk, 21-22, (1988)
  14. Mamatha D.N., Nagalakshmma K. and Rajeshwara Rao M., Impact of selected Juvenile Hormone Mimics on the organic constituents of silk worm, Bombyx mori (L), (1999)
  15. Khade V.B. and Ganga V. Mhamane, Vividh Vanaspati Arkancha Tuti Reshim Kitak Sangopanasathi Upyojana, Krishi Vdnyan, 4, 18-22 (2005)
  16. Khyade V.B., Poonam B., Patil M., Jaybhay Rasika R., Gaikwad Ghantaloo U.S., Vandana D. Shinde, Kavita H., Nimbalkar and Sarwade J.P., Use of digoxin for improvement of economic parameters in silk worm, Bombyx mori (L), Bioinfrmatics (Zoological Society of India), (2007)
  17. Krishnaswami S., Narasimhana M.N., Suryanarayana S.K. and Kumaraj S., Sericulture Manual–11 : Silk worm Rearing. F A O, United Nation’s Rome, 131 (1978)
  18. Riddiford L.M., Hormone action at cellular level. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, G. A. Kerkut and L.I. Gilbert (Eds.), 8, 37-64 (1985)
  19. Riddiford L.M., Cellular and molecular actions of Juvenile hormone: General consideration and premeta- morphic actions, J. Adv. Insect Physiology, 24, 213-214 (1994)
  20. Sehnal F. and Rambold H., Brain stimulation and juvenile hormone production in insect larvae. Experentia. 44, 684-685 (1985)
  21. Norman, T. J. and Baily (1955) Statistical Methods
  22. Omana Joy and Shyamala M.B., Non-spinning syndrome in silk worm: Occurrence and Pathology. Research paper presented for National Seminar on silk research and development, Banglore, India, (1983)
  23. Prabhu V.K.K., John M. and Ambika B., Juvenile hormone activity in some south Indian plants, Current Science, 42, 72-726 (1973)
  24. Slama K., Plant as source material with insect hormone activity. Ent. Exp. Appl., 12, 721-728 (1969)
  25. Slama K., Insect juvenile hormone analogues. Ann. Rev. Biochem. 40, 1079-1102 (1971)
  26. Slama K., Romanuk M. and Sorm F., Insect hormones and Bioanalogues. Springer Verlag, Wein and New York, (1974)
  27. Slama K., Pharmacology of Insect Juvenile Hormones. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, (Eds. G. A. Kerkut and L. I. Gilbert). 11, 357-394 (1985)
  28. Williams C.M., The Juvenile Hormone of Insects, Nature, 178, 212-213 (1956)