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In vitro Antifungal activities of Essential oils extracted from Fresh Leaves of Cinnamomum zeylanicum and Ocimum gratissimum against Foodborne pathogens for their use as Traditional Cheese Wagashi conservatives

Author Affiliations

  • 1Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, BENIN
  • 2 Laboratoire d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, 01 BP 2009 Cotonou, BENIN
  • 3 Laboratoire de Microbiologie et de Biotechnologie Alimentaire, Faculté des Sciences Agronomiques, 01 BP 526 Cotonou, BENIN

Res. J. Recent Sci., Volume 1, Issue (9), Pages 67-73, September,2 (2012)


Alternative natural additives are needed in order to guarantee food safety in preservation against foodborne pathogens. Aromatic plants are traditionally employed for seasoning and prolongation of shelf life of food. The majority of their properties are due to the essential oils produced by their secondary metabolism. The objective of this study was to assess in vitro antifungal activity of essential oils extracted from fresh leaves of Cinnamomum zeylanicum and Ocimum gratissimum obtained by hydrodistillation with Clevenger apparatus against spoilage and pathogens moulds Aspergillus terreus, Aspergillus ustus, Aspergillus niger, Aspergillus aculeatus, Penicillium brevicompactum and Scopulariopsis brevicaulis both isolated from wagashi. The screening of their antifungal activity was carried out by determination of antifungal activity parameters as mycelial growth inhibition, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Results obtained from this study showed that Ocimum gratissimum essential oil was the most effective as antifungal agent among the essential oils tested due probably to its prominent concentration in phenolic compound thymol with MIC ranged from 600 to 800 mg/L. Scopulariopsis brevicaulis and Aspergillus terreus were the most sensible strains with minimum fungicidal concentration (MFC) varying from 600 to 1000 mg/L while Aspergillus aculeatus was the most resistant mould of all to essential oil of cinnamon. With the rise of this study, it is shown that Cinnamomum zeylanicum and especially Ocimum gratissimum essential oils could be regarded as a very promising preservative for wagashi in order to prevent the mycelia growth responsible of its deterioration.


  1. Barkat M. and Bouguerra A., Study of the antifungal activity of essential oil extracted from seeds of Foeniculum vulgare Mill. for its use as food conservative, Afr. J. Food Sci., 6(9), 239-244 (2012)
  2. Agbangnan D.C.P., Tachon C., Dangou J., Chrostowska A., Fouquet E. and Sohounhloue K.C.D., Optimization of the Extraction of Sorghum's Polyphenols for Industrial Production by Membrane Processes, Res. J. Recent Sci., 1(4), 1-8 (2012)
  3. Kèkè M., Yèhouénou B., Dahouénon E., Dossou J. and Sohounhloué K.C.D., Contribution à l’amélioration de la technologie de fabrication et de conservation du fromage peulh waragashi par injection de Lactobacillus plantarum, Ann. Sci. Agron.Bénin.,10(1)73-86 (2008)
  4. Aïssi V.M., Soumanou M.M., Bankolé H., Toukourou F.and de Souza C.A., Evaluation of Hygienic and Mycological Quality of Local Cheese Marketed in Benin, AJBAS., 3(3), 2397-2404 (2009)
  5. Filtenborg O., Frisvad J.C. and Thrane U., Moulds in food spoilage, Int. J. Food Microbiol., 33, 85-102 (1996)
  6. Nasser A.L., Fungal contamination of white cheese at the stage of consumption in Saudi Arabia, Pak. J. Biol. Sci., 4(6), 733-735 (2001)
  7. Abedi-Tizaki M., Askari A., Paknezhad H. and Abedi M.R., Rapid Detection Methods for Analysis of Fungi and Mycotoxins in Agriculture Products, Res. J. Recent Sci., 1(7), 90-98 (2012)
  8. Nakade Dhanraj B., Antibiotic sensitivity of Common Bacterial Pathogens against selected Quinolones, ISCA J. Biological Sci., 1(1), 77-79 (2012)
  9. Chahardehi A.M., Ibrahim D., Sulaiman S.F., Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla, Int. J. Microbiol., ID 826830, (2010)
  10. Hegde Chaitra R., Madhuri M., Swaroop T., Nishitha T., Das A., Bhattacharya S. and Rohit K.C., Evaluation of antimicrobial properties, phytochemical contents and antioxidant capacity of leaf extracts of Punica granatum L., ISCA J. Biological Sci., 1(2), 32-37 (2012)
  11. Ogbe A.O. and John P.A., Effect of Polyherbal Aqueous Extracts (Moringa oleifera, Gum arabic and wild Ganoderma lucidum) in Comparison with Antibiotic on Growth Performance and Haematological Parameters of Broiler Chickens, Res. J. Recent Sci., 1(7), 10-18 (2012)
  12. Wang H.F., Yih K.H. and Huang K.F., Comparative Study of the Antioxidant Activity of Forty-five Commonly Used Essential Oils and their Potential Active Components, J. Food Drug Anal., 18(1), 24-33 (2010)
  13. Angelini P., Pagiotti R., Menghini A. and Vianello B., Antimicrobial activities of various essential oils against foodborne pathogenic or spoilage moulds, Ann. Microbiol., 56(1)65-69 (2006)
  14. Nwinyi O.C., Chinedu N.S., Ajani O.O., Ikpo C.O. and Ogunniran K.O., Antibacterial effects of extracts of Ocimum gratissimum and piper guineense on Escherichia coli and Staphylococcus aureus, Afr. J. Food Sci., 3(1), 22-25 (2009)
  15. Matasyoh L.G., Matasyoh J.C., Wachira F.N., Kinyua M.G., Thairu Muigai A.W. and Mukiama T.K., Antimicrobial Activity of Essential Oils of Ocimum gratissimum L. from different populations of Kenya, Afr. J. Trad. CAM., 5(2), 187-193 (2008)
  16. Oussou K. R., Yolou S. F., Tue Bi B., Kanko C., Boti J. B., Ahibo C. and Casanova J., Etude chimique bio-guidée de l’huile essentielle de Ocimum gratissimum (Lamiaceae), Eur. J. Sci. Res., 40(1), 50-59 (2010)
  17. Simic A., Sokovic M. D., Ristic M., Grujic-Jovanovic S., Vukojevic J. and Marin P. D., The chemical composition of some Lauraceae essential oils and their antifungal activities, Phytother. Res., 18, 713-717 (2004)
  18. Unlu M., Ergene E., Unlu G.V., Zeytinoglu H.S. and Vural N., Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicumBlume (Lauraceae), Food Chem Toxicol. 48, 3274-3280 (2010)
  19. Sessou P., Farougou S., Alitonou G.A., Djenontin T.S., Yehouenou B., Azokpota P., Youssao I. and Sohounhloue D., Chemical composition and antifungal activity of essential oil of fresh leaves of Ocimumgratissimum from Benin against six mycotoxigenic fungi isolated from a traditional cheese wagashi, ISCA J. Biological Sci., article in press ID ISCA-JBS-093 (2012)
  20. Sessou P., Farougou S., Noudogbessi J.P., Yehouenou B., Azokpota P., Youssao I., Sohounhloue D., Chemical composition and antifungal activity of essential oil of fresh leaves of Cinnamomum zeylanicum from Benin against six mycotoxigenic fungi isolated from a traditional cheese wagashi., Elixir J. AppliedBiol., article in press ID Elixir_2012061378 (2012)
  21. Yèhouenou B., Noudogbèssi J.P., Sessou P., Avlessi F. and Sohounhloué D., Etude chimique et activités antimicrobiennes d’extraits volatils des feuilles et fruits de Xylopia aethiopica (DUNAL) A. Rich. contre les pathogènes des denrées alimentaires, J. Soc. Ouest Afr. Chim., 29, 19-27 (2010)
  22. ISO 21527-1 : 2008 (F), Microbiologie des aliments-Méthode horizontale pour le dénombrement des levures et moisissures, Partie 1 : technique par comptage des colonies dans les produits à activité d’eau supérieure à 0,95, 1èreédition (2008)
  23. Samson A., Hoekstra E., Frisvad J.C., Filtenborg O., Introduction to food-borne fungi. Copyright 1995 Centraalbureau voor Schimmelcultures ISBN 90-70351-27-7, 322 (1995)
  24. Pitt J.I., Hocking A.D., Fungi and Food spoilage, Springer Dordrecht Heidelberg London New York, USA, 518 (2009)
  25. Tatsadjieu N.L., Dongmo Jazet P.M., Ngassoum M.B., Etoa F-X. and Mbofung C. M. F.,Investigations on the essential oil of Lippia rugosa from Cameroun for its potential use as antifungal agent against Aspergillus flavus Link ex Fries, Food Control,20, 161-166 (2009)
  26. Statistica, Statistica version 2010 monoposte 6.0 (2010)
  27. Lopez-Reyes J.G., Spadaro D., Gullino M.L. and Garibaldi A., Efficacy of plant essential oils on postharvest control of rot caused by fungi on four cultivars of apples in vivo, Flavour Fragr. J., 25, 171-177 (2010)
  28. Burt S., Essential oils: their antibacterial properties and potential applications in food-areview, Int. J. Food Microbiol., 94, 223-253 (2004)
  29. Murray P.R., Baron E.J., Jorgensen J.H., Pealler A.M. and Yolken R. H., Clinical Microbiology 8th Edition, (2003)
  30. Lambert R.J.W., Skandamis P.N., Coote P. and Nychas G. J.E., A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol, J. Appl. Microbiol., , 453-462 (2001)
  31. Mangale Sapana M., Chonde Sonal G., Raut P.D., Use of Moringa Oleifera (Drumstick) seed as Natural Absorbent and an Antimicrobial agent for Ground water Treatment, Res. J. Recent Sci.,1(3), 31-40(2012)
  32. Ahonkhai I., Ayinde B.A., Edogun O. and Uhuwmangho M.U., Antimicrobial activities of the volatile oils of Ocimum bacilicum L. and Ocimum gratissimum L. (Lamiaceae) against some aerobic dental isolates, Pak. J. Pharm. Sci., 22(4), 405-409 (2009)
  33. Prakash B., Shukla R., Singh P., Mishra P.K., Dubey N.K. and Kharwar R.N., Efficacy of chemically characterized Ocimum gratissimum L. essential oil as an antioxidant and a safe plant based antimicrobial against fungal and aflatoxin contamination of spices, Food Res. Int., 44, 385-390 (2011)
  34. Nguefack J., Lekagne Dongmo J.B., Dakole C.D., Leth V., Vismer H.F., Torp J., Guemdjom E.F.N., Mbeffo M., Tamgue O., Fotio D., Amvam Zollo P.H. and Nguefack A. E., Food preservative potential of essential oils and fractions from cymbopogon citratus, Ocimum gratissimumand Thymus vulgaris against mycotoxigenic fungi, Int. J. Food Microbiol., 131, 151-156 (2009)
  35. Faria T.J., Ferreira R.S., Yassumoto L., Pinto de Souza J. R., Ishikawa N. K. and Barbosa A. M., Antifungal activity of essential oil Isolated from Ocimum gratissimum L. (eugenol chemotype) against Phytopathogenic Fungi, Braz. Arch. Biol. Technol., 49(6), 867-871 (2006)
  36. Ooi L.S. M., Li Y.L., Kam S.L., Wang H., Wong E.Y.L. and Ooi V.E.C., Antimicrobial activities of Cinnamom oil and cinnamaldehyde from the Chinese medical herb Cinnamomum cassia Blume, Am. J. Chin. Med., 34(3), 51-522 (2006)
  37. Shahverdi A.R., Monsef-Esfahani H.R., Tavasoli F., Zaheri A. and Mirjani R., Trans-cinnamaldehyde from Cinnamomum zeylanicum bark essential oil reduces the clindamycin resistance of Clostridium difficile in vitro, J. Food Sci., 72(1), 555-558 (2007)
  38. Diallo D., Ethnopharmacological survey of medicinal plants in mali and phytochemical study of four of them: Glinus oppositifolius (Aizoaceae), Diospyros abyssinica(Ebenaeceae), Entada Africana (Minosaceae), Trichilia emetic (Meliaceae), These of doctorat, University of Lausanne, Suisse, 221 (2000)