6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

The effects of IBA and 2ip on callogenesis and shoot formatting of Cymbidium orchid var "Red Tiffani"

Author Affiliations

  • 1 Young Researchers Club, Bandar Anzali Branch, Islamic Azad Univercity, Bandar Anzali, IRAN

Res. J. Recent Sci., Volume 1, Issue (8), Pages 70-72, August,2 (2012)

Abstract

Cymbidium orchid var “Red Tiffani” is one commercial orchid flower that propagated with micropropagation method. For study on effect of IBA and 2ip on micropropagation of Cymbidium orchid var “Red tiffani”, four Concentrations of IBA (0, 1, 2 and 4 mg l-1) and four Concentration of 2ip (0, 1, 2 and 4 mg l-1) in three replications were examined. Explants selected on young leaf and after sterilization, for callus induction transport in MS medium with IBA and 2ip. After 5 week, calli transport in new same medium for shoot induction. In this study traits such as days of callus initiation, percent of callus, number of shoot per explants, shoots fresh weight, shoot length were examined. Result showed that the IBA and 2ip effect of callus and shoot traits respectively.

References

  1. Hossain M.M., Sharma M. Teixeira da Silva J.A. and Pathak P., Seed germination and tissue culture of Cymbidium giganteum Wall, ex Lindl, Sci. Hortic, 123, 479-487 (2010)
  2. Nick W.A., Arathoon S., Collettc V.E. Schwinn K.E.,Jameson P.E., Lewis D.H., Zhang H. and Davies K.M.,Activation of anthocyanin synthesis in Cymbidium orchids: variability between known regulators, Plant Cell Tiss Organ Cult, 100(3), 355- 360 (2010)
  3. Silvia A., Pinheiro Moreira F. and Mary dos Santos Isaias R., Comparative anatomy of the absorption roots of terrestrial and epiphytic orchids, Brazilian Archives of Biology and Technology, 51(1), 83-93 (2008)
  4. Dearnaley J., Further advances in orchid mycorrhizal research, Mycorrhiza, 17(6), 475-486 (2007)
  5. Partomihardjo T., Colonisation of orchids on the Krakatau Islands,Telopea, 10(1), 299-310 (2003)
  6. Kostenvuk I., Oh B.J. and So I.S., Induction of early flowering in Cymbidium niveo-marginatum mak in vitro,Plant Cell Reports, 19(1), 1-5 (1999)
  7. Buyun L., Lavrentyeva A., Kovalska L. and Ivannikov R., In vitro germination of seeds of some rare tropical Orchids, Acta Universitatis Latviensis, Biology, 676, 159–162 (2004)
  8. Kumar U., Methods in plant tissue culture, Second Edition, Agrobios Jodhpur, India (2003)
  9. Vujanovic V., St-Arnaud M., Barabea D. and Ve Thibeault G., Viability testing of orchid seed and the promotion of colouration and germination, Annals of Botany,86, 79-86 (2000)
  10. Batygina T.B., Bragina E.A. and Vasilyeva V.E., The reproductive system and germination in orchids, Acta Biologica Cracoviensia Series Botanica,45(2), 21–34 (2003)
  11. Chen T., In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies, Acta Physiologiae Plantarum, Online (2012)
  12. Chyuam-Yih Ng and Mohd Saleh, In vitro propagation of Paphiopedilum orchid through formation of protocormIike bodies, Plant Cell Tiss Organ Cult., 105,193-202 (2011)
  13. Panwar D., Ram1 K., Harish N. and Shekhawat S., In vitropropagation of Eulophia nuda Lindl., an endangered orchid, Scientia Horticulturae, 139, 46-59 (2012)
  14. Shasany A.K., Khanuja S.P.S., Dhawan S., Yadav V., Sharma S. and Kumar S., High regenerative nature of Mentha arvensis internodes, J. Biosci, 23, 641-646 (1998)
  15. Lim W.L. and Loh C.S., Endopolyploidy in Vanda miss joaquim (Orchidaceae), New Phytol, 159(9), 279-287 (2003)
  16. Gow W.P., Chen J.T. and Chang W.C., Influence of growth regulators on direct embryo formation from leaf explants of Phalaenopsis orchids,Acta Physiologiae Plantarum, 30(4), 507-512 (2008)
  17. Jahan M.T., Islam M.R. Khan R., Mamun A.N.K. Ahmed G. and Hakim L. In vitro clonal propagation of anthurium Anthurium andraeanum L.) using callus culture, Plant Tissue Cult. & Biotech, 19(1), 61-69 (2009)
  18. Gopalakrishnan M., Janarthananm B., Lakshmi Sai G. and Sekar T., Plant regeneration from leaf explants of Plumbago rosea L., Plant Tissue Cult and Biotech, 19(1), 79-87 (2009)
  19. Juan L., Lihua W., jing L. and Junhui W., Effect of different plant growth regulators on callus induction in Catalpa bungei,African Journal of Agricultural Research, 5(19), 2699-2704 (2010)
  20. Lim W.L. and Loh C.S., Endopolyploidy in Vanda missjoaquim (Orchidaceae), New Phytol, 159(9), 279-287 (2003)
  21. Behera K.K. and Sahoo S., Rapid invitro micropropogation of sugarcane (Saccharum officinarum) through callus culture, Nature and Science,, 146-147 (2009)
  22. Yew C.K., Balakrishnan B., Sundasekaran J. and Subramaniam S., The effect of cytokinins on in vitro shoot length and multiplication of Hymenocallislittoralis,Journal of Medicinal Plants Research, 4(24), 2641-2646 (2010)
  23. Eman A.A., Initiation of Pharmaceutical Factories depending on more Application of Biotechnology on some Medicinal Plants Review Article, Research Journal of Recent Sciences, 1, 398-404 (2012)
  24. Guang-jie Z., Zhan-bin W. and Dan W. In vitropropagation and ex vitro rooting of blueberry plantlets, Plant Tissue Cult and Biotech, 18(1), 187-195 (2008)
  25. Al-Sulaiman M.A. and Barakat M.N., In vitro shoot multiplication of Ziziphus spina-christi by shoot tip culture, African Journal of Biotechnology, 9(6), 850-857 (2010)
  26. Rasmussen H., Terrestrial Orchids: from Seed to Mycotrophic Plant, Cambridge University Press, Cambridge, (2005)
  27. Rout G.R., Samantaray S. and Das P., In vitromanipulation and propagation of medicinal plants, Biotechnology Adv, 18, 91-120 (2000)