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The effects of IBA and 2ip on callogenesis and shoot formatting of Cymbidium orchid var "Red Tiffani"

Author Affiliations

  • 1 Young Researchers Club, Bandar Anzali Branch, Islamic Azad Univercity, Bandar Anzali, IRAN

Res. J. Recent Sci., Volume 1, Issue (8), Pages 70-72, August,2 (2012)


Cymbidium orchid var “Red Tiffani” is one commercial orchid flower that propagated with micropropagation method. For study on effect of IBA and 2ip on micropropagation of Cymbidium orchid var “Red tiffani”, four Concentrations of IBA (0, 1, 2 and 4 mg l-1) and four Concentration of 2ip (0, 1, 2 and 4 mg l-1) in three replications were examined. Explants selected on young leaf and after sterilization, for callus induction transport in MS medium with IBA and 2ip. After 5 week, calli transport in new same medium for shoot induction. In this study traits such as days of callus initiation, percent of callus, number of shoot per explants, shoots fresh weight, shoot length were examined. Result showed that the IBA and 2ip effect of callus and shoot traits respectively.


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