9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Optimization of production conditions of lipase from B. licheniformis MTCC 10498

Author Affiliations

  • 1 Department of Biotechnology, Himachal Pradesh University, Shimla-171 005, INDIA

Res. J. Recent Sci., Volume 1, Issue (7), Pages 25-32, July,2 (2012)

Abstract

The initial broth containing 0.3 (%; w/v) yeast extract, 0.1% sodium nitrate (w/v) etc. were calibrated to a final pH of 7.5 to determine cumulative effect of all the selected components on lipase production by B. licheniformis MTCC 10498. The MB broth was autoclaved at 1.1 bar for 20 min. at 121C. This broth was inoculated with 10% (v/v) of 36 h old seed culture and incubated under shaking at 55C for 72 h. The inoculated MB broth was harvested at 72 h by centrifugation (10, 000 X g for 20 min. at 4C; Sigma 3K30, Germany). The supernatant was filtered through Whatman filter paper no. 1. This enzyme preparation was termed as crude lipase. The lipase produced by B. Licheniformis MTCC-10498 in various batches was recorded. The pH was adjusted to 8.0 0.2 and the final volume was made to 1000 ml with sterile distilled water. This crude lipase was subjected to lipase assay and finally 2.0 U/ml activity were recorded.

References

  1. Treichel H., Olivera D.D., Mazutti M.A., Luccio M.D. and Olivera J.M., A Review on Microbial Lipases Production Food. Bioproc. Technol.,, 182-196 (2010)
  2. Sangeetha R., Arulpandi I. and Geetha A., Bacterial Lipases as Potential Industrial Biocatalysts: An Overview, Res. J. Microbiol.,, 1-24 (2011)
  3. Rani C. and Panneerselvam A., Influence of Environmental and Nutritional Parameters on Lipase Production, J Agric. Biol. Sci.,4(5), 39-43 (2009)
  4. Winkler U.K. and Stuckmann M., Glucogen, Hyaluronate and Some Other Polysaccharides Greatly Enhance the Formation of Exolipase by Serratia marcescens, J. Bacteriol.,138, 663-670 (1979)
  5. Wetlaufer, D.B., Ultra Violet Spectra of Proteins and Amino Acids, Adv. Prot. Chem., 17, 303-391(1962)
  6. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the Folin-Phenol reagents, J. Biol. Chem.,193, 265 (1951)
  7. Lau H.L., Arif A., Woo K.K., Ling T.C. and Hii S.L., Production and Optimization of Alkalostable Lipase by Alkalophilic Burkholderia cenocepacia ST-8, Afr. J. Biotechnol.,10, 7002-7009 (2011)
  8. Kanwar S.S., Ghazi I.A. and Chimni S.S., Purification and Properties of a Noble Extra-cellular Thermotolerant Metallolipase of Bacilluscoagulans MTCC-6375 Isolate. Protein. Expr. Purif. 46, 421-428 (2006)
  9. Wang Y., Srivastava K.C., Shen G-J., and Wang H.Y., Thermostable Alkaline Lipase from A Newly Isolated Thermophilic Bacillus Strain A30-1 (ATCC 53841), J. Ferment. Bioeng.,79, 433-438 (1995)
  10. Ghanem E.H., Al-Sayeed H.A., and Saleh K.M., An Alkalophilic Thermostable Lipase Produced by A New Isolate of Bacillusalcalophilus,World. J. Microbiol. Biotechnol.,16, 459-464 (2000)
  11. Lanser A.C., Manthey L.K. and Hou C.T., Regioselectivity of New Bacterial Lipases Determined By Hydrolysis of Triolein, Curr. Microbiol.,44, 336-340 (2002)
  12. Sharma R., Soni S.K., Vohra R.M., Gupta L.K. and Gupta J.K., Purification and Characterization of A Thermostable Alkaline Lipase from A New Thermophilic Bacillus sp. RSJ-1, Process Biochem, 37, 1075-1084 (2002)
  13. Gupta R., Gupta N. and Rathi P., Bacterial lipases: An Overview of Production, Purification and Biochemical Properties, Appl. Microb. Biotechnol.,64, 763-781. (2004)
  14. Papaparaskevas D., Christakopoulos P., Kekos D., Macris B.J., Optimizing Production of Extracellular Lipase from Rhodotorula glutinis,Biotechnol. Lett.,14, 397402 (1992)
  15. Vakhlu J and Kaur A., Yeast Lipases: Enzyme Purification, Biochemical Properties and Gene Cloning, Electron, J. Biotechnol.,, 69-85 (2006)
  16. Sharma C.K., Kanwar, S.S. and Chauhan G.S., Synthesis of medically important ester ethyl cinnamate by porcein pancreatic lipase immobilized on Poly (AAc-co-HPMA-cl-EGDMA) hydrogel, J. Appl. Polym. Sci., 121, 26742679 (2011)
  17. Verma M.L., Azmi W. and Kanwar S.S., Microbial lipases: At the interface of aqueous and non-aqueous media-A review, Acta Microbiol Immunol Hung,55, 265-293, (2008)
  18. Gupta N., Shai V. and Gupta R., Alkaline Lipase from A Novel Strain Burkholderia multivorans: Statistical Medium Optimization and Production in a Bioreactor, Proc. Biochem.,42, 518-526 (2007)
  19. Kulkarni N. and Gadre R.V., Production and Properties of An Alkaline, Thermophilic Lipase from Pseudomonas fluorescens NS2W, J. Ind. Food. Microbiol.,28, 344-348 (2002)
  20. Wei G., Yu J., Zhu Y., Chen W. and Wang L., Characterization of Phenol Degradation by Rhizobium sp. CCNWTB 701 Isolated from Astragalus chrysopteru in Mining Tailing Region, J. Hazard. Mater.,151, 111-117 (2008)
  21. Kiran G.S., Shanmughapriya S., Jayalakshmi J., Selvin J., Gandhimathi R., Sivaramakrishnan S., Arunkumar M., Thangavelu T. and Natarajaseenivasan K., Optimization of Extracellular Psychrophilic Alkaline Lipase Produced by Marine Pseudomonas sp. (MSI057), Bioprocess Biosyst. Eng.,31, 483-492 (2007)
  22. Bradoo S., Saxena R.K. and Gupta R., Two acidothermotolerant lipases from new variants of Bacillusspp. , World. J. Microbiol. Biotechnol.,15, 87-91 (1999)
  23. Lithauer D., Ginster A., Skein E., Pseudomonas luteola lipase: A New Member of the 320 Residue Pseudomonas Lipase Family, Enz. Microbial Technol., 30, 209-215 (2002)
  24. Lin S.F., Chiou C.M. and Tsai Y.C., Effect of Triton X-100 on Alkaline Lipase Production by Pseudomonaspseudoalkaligenes F-111, Biotechnol Lett17, 959-962 (1995)
  25. Patkar S.A. and Bjorkling F., Lipase Inhibitors. In: Woolley P, Petersen SB (eds) Lipases-Their Structure, Biochemistry and Application, Cambridge University Press, Cambridge, 207-224 (1994)
  26. Wang C.S., Dashti A. and Downs D., Bile Salt-Activated Lipase, Meth. Mol. Biol., 109, 7179 (1999)
  27. Kanwar S.S., Sharma C.K., et al., Short Chain Ester Synthesis by Transesterification Employing Poly (MAc-co-DMA-cl-MBA) Hydrogel Bound Lipase of Bacillus coagulans MTCC-6375, J. Appl. Polym. Sci., 109, 1063-1071 (2008)