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Antimitotic activity of a New Compound Isolated from the Flower of Prosopis juliflora

Author Affiliations

  • 1 Center for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, INDIA

Res. J. Recent Sci., Volume 1, Issue (6), Pages 22-26, June,2 (2012)

Abstract

A new compound was isolated from the flower of Prosopis juliflora and identified by mass, IR and NMR techniques. Its antimitotic activity was evaluated with the help of allium test. Onion root tips were subjected with 4 and 8 mg/ml concentrations of the compound, up to 48 hours for studying its effect on root mitosis. The roots were examined in permanent root tip squash preparations stained by the aceto-carmine. The results obtained confirmed that the compound have various effects on chromosomes and induced different mitotic abnormalities and structural aberration of chromosomes. Various chromosomal aberrations such as clumping and stickiness, fragmentation, C-mitotic effect, anaphase bridge were observed, which clearly showed the clastogenic, antimitotic and cytotoxic effect.

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