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Synthesis of methyl cinnamate using immobilized lipase from B. licheniformis MTCC-10498

Author Affiliations

  • 1 Department of Biotechnology, Himachal Pradesh University, Shimla-171005, HP, INDIA

Res. J. Recent Sci., Volume 1, Issue (3), Pages 68-71, March,2 (2012)

Abstract

Methyl cinnamate was synthesized by the reaction of methyl alcohol with cinnamic acid using silica immobilizedlicheniformis MTCC-10498. The silica-bound lipase was used to perform esterification of methanol with cinnamic acid in equimolar ratio (100 mM each) in DMSO. Moreover, pure methyl cinnamate was used as internal standard. Methyl cinnamate was synthesized by manipulating various parameter i.e. relative proportions of reactants, reaction temperature, reaction timeconcentration of molecular sieves and salt ions. Finally, 64.3 mM methyl cinnamate was synthesized at 65°C using 100mM each of the reactants in 12 h in the presence of molecular sieves (100 mg/reaction volume) under shaking at 120 rpm. The immobilized lipase synthesized 50 % ester u

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