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Protein contents and activity of enzymes in the mod gut homogenate of fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) fed with herbal drug(Kho-go) treated mulberry leaves

Author Affiliations

  • 1Department of Zoology, Shardabai Pawar Mahila College, Shardanagar Tal – Baramati, Dist. Pune–413115, MH, INDIA
  • 2 Department of Zoology, T.C. College, Baramati, Dist. Pune, MH, INDIA

Res. J. Recent Sci., Volume 1, Issue (2), Pages 49-55, February,2 (2012)

Abstract

The aqueous solution of herbal drug: kho-go of various concentrations (5.0 ppm; 10.0 ppm; 20.0 ppm and 50.0 ppm) was used to treat the leaves of mulberry and fed to the fifth instar larvae of polyvoltine, crossbreed silkworm, Bombyx mori (L) for first three days, second day and third day and third day (only). The larvae fed with untreated and water treated leaves were also maintained. Bioassays of proteins (S.P. and T.P.) and enzymes (protease and amylase) were carried out on fifth day through the use of mid gut homogenate. Treating the mulberry leaves with herbal drug: kho-go and feeding them to fifth instar larvae was found reflected into significant improvement in the levels of proteins (S.P. and T.P.) and velocities of biochemical reactions catalyzed by protease and amylase. The pattern of increase in soluble proteins and total proteins in the mid gut tissue were 32.147 to 90.074 percent and 5.657 to 39.052 percent respectively. The activities of mid gut protease and amylase were increased by 21.444 to 83.706 percent and 14.54 to 52.257 percent respectively. The nutrient contents of herbal drug: kho-go serve to improve the digestibility and exert the influence of efficient metabolism in the fifth instar larvae of silkworm, Bombyx mori (L). The herbal drug treatment may gear overall biochemical constituency of silkworm larvae, through mid gut enzymes.

References

  1. Bowers W.S., Fales V.M., Thompson M.J. and Uebel B., Juvenile and gonadotropic activity of 10, 11 epoxyfranesoic acid methyl ester, Life Science,, 2323-2331 (1966)
  2. Murugan K. and George A. (Sr.), Feedings and nutritional influence on growth and reproduction of Daphnia near (L), Insect Physiol., 38, 961-969 (1992)
  3. Ito H., Effect of sugars on feeding the larvae of silkworm, Bombyx mori (L), J. Insect. Physiol.,5, 95-107 (1960)
  4. Ito T., Nutrition of silkworm, Bombyx mori (L). Proc. Jpn. Acad. Sci. 43, 57-61 (1961)
  5. Nayar J.K. and Frankel G., Journal of Insect Physiology,, 505 (1962)
  6. Ito T., Kwashima K., Nakhara M., Nakanshi K. and Terahara A., Metabolism in the mid gut of silkworm, Bombyx mori (L), Insect Physiol.,10, 225-228 (1964)
  7. Murali K., Effect of leaf carbohydrate reserves on the growthand excretory pattern of silkworm, Bombyx mori (L), M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati, India (1992)
  8. Subramanyam Reddy C., Studies on distribution of digestive enzymes in the digestive tract of silkworm, Bombyx mori (L), M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati India (1992)
  9. Slansky F. and Scriber J.M. Food consumption and Utilization, In: comprehensive Insect physiology, Biochemistry and pharmacology, (Eds. Kerkut G.A. and Gilbert L.I.) Pergamon Press, Oxford, 4, 639 (1985)
  10. Horie Y., Tanaka M. and Ito T., Proteolytic enzyme of digestive juice of mid gut in silkworm, Bombyx mori (L). J. Setricult. Sci., Japan, 32, 8-15 (1963)
  11. Yamafuji, I. and Yonezawa(1935)
  12. Lipases in silkworm, Bombyx mori (L).Insect. Biochem. 1: 102-112 Vitthalrao B. Khyade and Jyoti Kulkarni, Effect of digoxin treated mulberry leaves on protein profiles in fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR). Res. J. Chem. Sci., 1(1): 2-7 (www.isca.in) (2011)
  13. Desai V.A., Pawar V.V. and Sawant R.T., Influence of herbal drug: kho-go on the fifth instar larvae of silkworm, Bombyx mori (L). Dissertation in the partial fulfillment of M.Sc. (Microbiology), Shardabai Pawar Mahila College, Shardanagar (Baramati), (Pune University, Pune) (2011)
  14. Krishnaswami S., Narasimhna M.N., Suryanarayana S.K. and Kumararaj S., Sericulture Manual-II Silkworm Rearing: FAO. United Nations Rome (1978)
  15. Vitthalrao Khyade and Jeevan P. Sarawade, Protein profiles in the fifth instar larvaeof silkworm, Bombyx mori (L)(PM xCSR) fed with digoxin treated mulberry leaves. The Bioscan, (1), 41-44 (2009)
  16. Khyade V.B., Influence of juvenoids on silkworm, Bombyx mori (L), Ph.D. thesis, Shivaji University, Kolhapur (2004)
  17. Brik Y., Harpaz J., Ishaya and Bhondi A., Studies on proteolytic activity of beetle, Tenebrio molitor (L), J. Insect Physiol., 8, 417-429 (1962)
  18. Ishaya I. and Swirski E., Trehalase invertase and amylase activities in the larvae of Egyptian cotton worm, Spodoptera littoralis (L), J. Insect Physiol.,17, 945-953 (1976)
  19. Chougale A.C., Influence of magnetic energy on silkworm Bombyx mori (L). Ph.D. Thesis, Shivaji University, Kolhapur (1992)
  20. Bernfeld P., Amylase, a and b. In: Methods of Enzymology, Vol. I (Ed. Clockwik and Kalpin). Academic Press, New York, (1955)
  21. Ishaaya I., Moore I. and Joseph B., Protease and amylase activity in the larvae of Egyption cotton worm, Spodoptera littoralis (L), J. Insect physiol.17, 945-953 (1971)
  22. Gaikwad A.R., Biology of some dung beetles of South Western Maharashtra. Ph.D. thesis, Shivaji University, Kolhapur (1998)
  23. Norman T.J. and Baily Statistical methods in Biology (1955)
  24. Applebaum S.W., Biochemistry of digestion. In: Comprehensive insect physiology (Ed. Kerkut, G.A. and Gilbert (I), 4:297-307, Pergamon Press New York, (1985)
  25. Ghantaloo U.S. Influence of digoxin on silkworm, Bombyx mori (L). M.Phil. Thesis, Algappa University, Karaikudi (Tamil Nadu), India (2007)
  26. Horie Y., Physiological studies on the limentary canal of silkworm, Bombyx mori (L), III. Absoroption and utilization of carbohydrates, Bull. Sericult. Exp. Sta., Japan, 16, 287-309 (1961)
  27. Jagtap S.G., Effect of plant juvenoids on consumption and utlizationof mulberry leaves by silkworm, Bombyx mori (L), M.Phil. Thesis, Algappa University, Karaikudi, Tamil Nadu, India (2007)
  28. Lowery O.H., Rosenbrough N.J., Far A.L. and Randall R.J. Protein measurement with folin phenol reagent,J. Biol. Chem., 193: 265-275 (1951)