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Protein contents and activity of enzymes in the mod gut homogenate of fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) fed with herbal drug(Kho-go) treated mulberry leaves

Author Affiliations

  • 1Department of Zoology, Shardabai Pawar Mahila College, Shardanagar Tal – Baramati, Dist. Pune–413115, MH, INDIA
  • 2 Department of Zoology, T.C. College, Baramati, Dist. Pune, MH, INDIA

Res. J. Recent Sci., Volume 1, Issue (2), Pages 49-55, February,2 (2012)


The aqueous solution of herbal drug: kho-go of various concentrations (5.0 ppm; 10.0 ppm; 20.0 ppm and 50.0 ppm) was used to treat the leaves of mulberry and fed to the fifth instar larvae of polyvoltine, crossbreed silkworm, Bombyx mori (L) for first three days, second day and third day and third day (only). The larvae fed with untreated and water treated leaves were also maintained. Bioassays of proteins (S.P. and T.P.) and enzymes (protease and amylase) were carried out on fifth day through the use of mid gut homogenate. Treating the mulberry leaves with herbal drug: kho-go and feeding them to fifth instar larvae was found reflected into significant improvement in the levels of proteins (S.P. and T.P.) and velocities of biochemical reactions catalyzed by protease and amylase. The pattern of increase in soluble proteins and total proteins in the mid gut tissue were 32.147 to 90.074 percent and 5.657 to 39.052 percent respectively. The activities of mid gut protease and amylase were increased by 21.444 to 83.706 percent and 14.54 to 52.257 percent respectively. The nutrient contents of herbal drug: kho-go serve to improve the digestibility and exert the influence of efficient metabolism in the fifth instar larvae of silkworm, Bombyx mori (L). The herbal drug treatment may gear overall biochemical constituency of silkworm larvae, through mid gut enzymes.


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