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In Silico Charecterization of Keratitis Causing Herpes Simplex Virus (HSV 1) Membrane Proteins using Computational Tools and Servers

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Author Affiliations

  • 1Center for Advanced Computing and Bioinformatics, CRD PRIST University, Thanjavur, Tamilnadu, INDIA
  • 2 Department of Biotechnology, FASC, MITS, Laxmangarh, Rajasthan, INDIA

Res. J. Recent Sci., Volume 1, Issue (11), Pages 27-31, November,2 (2012)

Abstract

Herpes viruses plays important role in the viral keratitis ocular infection almost all the HSV’s carrying the same virion morphology; icosahedral capsids containing the viral genome are surrounded by an amorphous layer of tegument, and this is encased in a lipid bilayer containing about a dozen different viral glycoprotein’s, 3D structure of proteins makes a pathways towards a drug designing and studies of drug interaction, they may have similar in sequences but differently in biological functions specially in case of the diseases. Present study focused on the characterization viral envelope proteins such as P04290, P06477, P04486 which having a great importance in the keratitis disease caused by HSV. Primary structure analysis shows that proteins are having high leucine residues with some cystein residues; Expasy Protparam studies inferred that all are unstable in nature; Secondary structure shows that some are predominate alpha helices with random coils. Transmembrane region prediction by SOSUI server predicted that P04290 and contain only one transmembrane region while P06477 soluble protein. Four transmembrane regions found in P04486 protein all predicted regions were analyzed by the helical plots using EMBOSS pepwheel 6.1, 3D structure identification done by using Swiss Model and structure validation has been done by PROCHECK and WHAT IF, such a modelled structures provides basic knowledge and good functional analysis for experimentally derived structures.

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