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Quantitative Determination of Selenium and Development of Chemical Sensing Indicator Plates via New Synthesized Dye and its Application in Water and Plant Samples

Author Affiliations

  • 1Department of Chemistry BUIT, Barkatullah University Bhopal, MP, INDIA
  • 2 Department of Chemistry, Sarojini Naidu Govt. Girls PG College, Bhopal, MP, INDIA
  • 3 Department of Chemistry BUIT, Barkatullah University, Bhopal, MP, INDIA

Res. J. Recent Sci., Volume 1, Issue (10), Pages 51-54, October,2 (2012)


A selective, simple, inexpensive and a new reagent for determination of selenium is proposed. This method is based on oxidation of hydroxylamine hydrochloride with selenite ions to nitrous acid, which in turn diazotizes sulfanilic acid which subsequently couples with NEDA to form magenta colored azo dye. The dye thus formed shows a maximum absorbance at 550 nm. The method obeys Beer’s law in the range of 0.05 to 0.26 ppm of Se. Its molar absorptivity, Sandell’s sensitivity, standard deviation and relative standard deviation were found 9.04x103, 8.73x10-3, 0.002, and 0.87% respectively. All the reaction parameters have been optimized. Interferences between the azo reaction and non targeted ions often present in environmental samples were investigated. The method has been successfully applied to the analysis of waste water and plant material. Chemical sensing strips were also been prepared and were successfully applied for detection of selenium in air and semi quantitative determination in water. The advantages of method are its high sensitivity, reproducibility, and the fact that measurement is simple, rapid and low cost.


  1. Murhekar G.H., Trace Metals Contamination of Surface Water Samples in and Around Akota City in Maharashtra, India, Res.J.Recent Sci., 1(7), 5-9(2012)
  2. Choudhary R., Heavy Metal Analysis of Water of Kaliasote Dam of Bhopal, MP, India, Res.J.Recent Sci., 1 (ISC-2011) , 352-353 (2012)
  3. Vaishnav V., Daga K., Chandra S. and Lal M., Adsorption Studies of Zn (II) ions from Wastewater using calotropis procera as an Adsorbent, Res.J.Recent.Sci., 1(ISC-2011) 160-165 (2012)
  4. Shapira J.R., in organic selenium compounds, Their Chemistry and Biology, Klayman D.L. and Gunther W.H., Wiley Interscience, New York, 701 (1971)
  5. Sitting M.I., Toxic metal, Pollution control and Worker Protection, Noyes Data Corporation, Park Ridge, USA (1976)
  6. Nwajei G.E., Okwagi P., Nwajei R.I. and Obi-Iyeke G.E., Analytical Assessment of Trace Elements in Soils, Tomato Leaves and Fruits in the Vicinity of Paint Industry, Nigeria,Res.J.Recent Sci.,1(4), 22-26 (2012)
  7. Patty F.A., Industrial Hygiene and forecasting, Wiley Interscience New York, , 886 (1962)
  8. Adhkins R.L., Walsh N., Edmunds M. and Trafford J.M., Inductively-Coupled Plasma-Atomic Emission Spectrometric Analysis of Low-Levels of Selenium in Natural-Waters, Analyst, 120, 1433-1436 (1995)
  9. Norheim G., Determination of Selenium in Biological Material using Automated Wet Digestion and an Automated Hydride Generation Atomic Absorption System,Application Note,Agilent Technologies © Agilent Technologies, Inc., 1988, November 1, (2010)
  10. Ramachandran K.N., Kaveeshwar R., and Gupta V.K., Spectrophotometric determination of selenium with 6amino-1-naphthol-3-sulphonic acid (J-acid) and its application in trace analysis,Talanta,40, 781 (1993)
  11. Johansson K., Luo X. and Olin A., Rapid one-step derivatization of Se(VI) to a piazselenol for the spectrofluorimetric determination of selenium in biological material,Talanta, 42, 1978 (1995)
  12. Ramanchandran K.N. and Kumar G.S., Modified spectrophotometric method for the determination of selenium in environmental and mineral mixtures using 2,3-diaminonaphthalene,Talanta,43(10), 1711-1715 (1996)
  13. Pursynska K.,Spectrophotometric Determination of Selenium with 1-Naphthyloamine-7-sulfonic Acid, Analytical Science,13(4), 629-632 (1997)
  14. Krishniah L.K., Kumar S., Suvardhan K. and Chiranjeevi P., Simple Spectrophotometric Determination of Traces of Selenium in Environmental Samples,3rd International Conference on Environment and Health, Chennai, York Uni, 217-225,15-17 Dec (2003)
  15. Subrahmanyam P., Krishnapriya B., Suvardhan K., Rekha D., Murali Krishna P., Rao G.C., Lingappa Y., Venkata Reddy B.C., Jayaraj B. and Chiranjeevi P., Spectrophotometric Determination of Se (IV) in Environmental Samples Using a Novel Oxidative Coupling Reagent, Environmental Monitoring and Assessment, 136(1-3), 1-7 (2005)
  16. Matamoros A. and Benning L.G., Spectrophotometric Determination of Low Level Concentrations of selenium in Aqueous Solutions, Mineralogical Magazine, 72, 451-454 (2008)
  17. Afkhami Aand Madrakian T., Kinetic–spectrophotometric determination of selenium in natural water after preconcentration of elemental selenium on activated carbon, Talanta, 58, 311-317 (2002)