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Determination of Diafenthiuron Residue in Orange pulp using a matrix Solid-Phase Dispersion Method Coupled to High-Performance Liquid Chromatography with Ultraviolet Detection

Author Affiliations

  • 1Department of Engineering Chemistry, Andhra University, Visakhapatnam, AP, INDIA
  • 2 SV University, Tirupathi, AP, INDIA
  • 3 Department of Engineering Chemistry, GITAM University, Hyderabad, AP, INDIA

Res. J. Recent Sci., Volume 1, Issue (10), Pages 55-58, October,2 (2012)


A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of diafenthiuron in orange pulp. The evaluated parameters included the type and amount of sorbent (silica gel and celite) and the nature of eluent (n-hexane, Acetonitrile and saturated aqueous sodium chloride solution). The best results were obtained using 1.0 g of orange pulp sample, 1.0 g of silica gel as sorbet and 20 mL of n-hexane - acetonitrile - saturated aqueous sodium chloride solution (1:1:1), (v/v)). The method was validated using orange pulp samples spiked with diafenthiuron at different concentration levels (0.03 and 0.3 g/mL). Average recoveries (using each concentration six replicates) ranged 88-94%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-2.0 g/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 g/mL and 0.03 g/mL respectively.


  1. Barker S.A., Long A.R., and Short C.R., Isolation of Drug residues from tissues by solid phase dispersion, J. Chromatogr., 475, 363-361 (1989)
  2. N.M. Brito., et al., Determination of pesticide residues in coconut water by liquid-liquid extraction and gas chromatography with electron-capture plus thermionic specific detection and solid-phase extraction and high-performance liquid chromatography with ultraviolet detection, Journal of Chromatography A,957, 201-209 (2002)
  3. Ho W. and Hsieh S.J., Solid phase micro extraction associated with microwave assisted extraction of organ chlorine pesticides in medicinal plants, Anal. Chim. Acta., 428111 (2001)
  4. Wang L., Zhao P., Zhang F., Du F., Pan C., Difenthiuron residue and decline in pakchoi and soil under field application, Ecotoxicol Environ saf., 78, 75-79 (2012)
  5. Zuin V.G., Yariwake J.H., Langas F.M., Analysis of pesticide residues in Brazilian plants, Braz.Chem.Soc, 14, 304-309 (2003)
  6. Inam., Reca I., Tekalp and Funda., Square wave volumetric determination of diafenthiuron and its application to water, soil and insecticide formulation, International journal of environmental and analytical chemistry., 92, 85-95 (2012)
  7. Navickiene. S., Aquino A., Bezerra. DS., A matrix solid-phase dispersion method for the extraction of seven pesticides from mango and papaya, J Chromatogr Sci.,48, 750-754 (2010)
  8. Abedi-Tizaki Mostafa., Askari Armin., Paknezhad Hamid and Abedi Mohammad Reza., Rapid detection methods for analysis of fungi and mycotoxins in agriculture products, Research journal of recent science., 1, 90-98 (2012)
  9. Rohilla S.K and Salar R.K., Isolation and characterization of various fungal strains from agricultural soil contaminated with pesticides, Research journal of recent science., 1(ISC-2011), 90-98 (2012)
  10. SANCO Guidelines., Method validation and quality control procedures for pesticide residues analysis in food and feed, Document NO. SANCO/10684/2009, (2009)