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Determination of Diafenthiuron Residue in Orange pulp using a matrix Solid-Phase Dispersion Method Coupled to High-Performance Liquid Chromatography with Ultraviolet Detection

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Author Affiliations

  • 1Department of Engineering Chemistry, Andhra University, Visakhapatnam, AP, INDIA
  • 2 SV University, Tirupathi, AP, INDIA
  • 3 Department of Engineering Chemistry, GITAM University, Hyderabad, AP, INDIA

Res. J. Recent Sci., Volume 1, Issue (10), Pages 55-58, October,2 (2012)

Abstract

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of diafenthiuron in orange pulp. The evaluated parameters included the type and amount of sorbent (silica gel and celite) and the nature of eluent (n-hexane, Acetonitrile and saturated aqueous sodium chloride solution). The best results were obtained using 1.0 g of orange pulp sample, 1.0 g of silica gel as sorbet and 20 mL of n-hexane - acetonitrile - saturated aqueous sodium chloride solution (1:1:1), (v/v)). The method was validated using orange pulp samples spiked with diafenthiuron at different concentration levels (0.03 and 0.3 µg/mL). Average recoveries (using each concentration six replicates) ranged 88-94%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg/mL and 0.03 µg/mL respectively.

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