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Significance of Blood Cellular lxr-α Gene Aberration in Coronary Heart Disease Subjects

Author Affiliations

  • 1Dept. of Experimental Medicine and Biotechnology, P. G. Institute of Medical Education and Research, Chandigarh, INDIA
  • 2 Dept. of Cardiology, P. G. Institute of Medical Education and Research, Chandigarh, INDIA

Res. J. Recent Sci., Volume 1, Issue (1), Pages 69-72, January,2 (2012)

Abstract

Keeping in view our previous finding that unambiguously revealed a significant positive correlation between the expression of mutated Liver X Receptor (LXR)- α gene and the extent of coronary heart disease (CHD), the present study was addressed to explore whether or not this observed blood cellular LXR- α gene aberration is pathognomonic feature of CHD. To detect previously reported blood cellular LXR-α gene aberration restriction digestion was performed with TaaI endonuclease in the LXR-α ligand binding domain derived from the cDNA library of peripheral blood mononuclear cells isolated from different unrelated inflammatory disease group (Rheumatic heart disease, Diabetes, Psoriasis and Tuberculosis ) including coronary heart disease. Inheritance of reported blood cellular LXR-α gene aberration was also checked in a family having a higher risk of CHD.Results of our study revealed that LXR-α gene aberration was not only selectively and specifically observed in the blood mononuclear cells derived from CHD patients but also showed a nonmendelian epigenetic inheritance in a family having higher risk of CHD. Based upon these results we propose that blood cellular LXR-α gene aberration may have the potential to act as a noninvasive marker for the early diagnosis of subjects that are at high risk of development of CHD.

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