Reverse Phase High Performance Liquid Chromatography method for Simultaneous Determination of Ofloxacin and Ornidazole in Pharmaceutical Dosage
- 1Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India
- 2Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India
Res.J.chem.sci., Volume 6, Issue (8), Pages 15-19, August,18 (2016)
For validation of ofloxacin and ornidazole from its combined pharmaceutical formulations a reverse phase high pressure liquid chromatography method is proposed. Resolution was carried out on Waters Symmetry shield C8 column. A mobile phase composed of acetonitrile and buffer (18:82% (v/v). Wavelength 280 nm was used for detection. The ICH guidelines was applied for study of different parameters. The proposed method is easily and successfully applied to analyze ofloxacin and ornidazole from pharmaceutical combined formulations.
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