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Reverse Phase High Performance Liquid Chromatography method for Simultaneous Determination of Ofloxacin and Ornidazole in Pharmaceutical Dosage

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Author Affiliations

  • 1Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India
  • 2Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India

Res.J.chem.sci., Volume 6, Issue (8), Pages 15-19, August,18 (2016)

Abstract

For validation of ofloxacin and ornidazole from its combined pharmaceutical formulations a reverse phase high pressure liquid chromatography method is proposed. Resolution was carried out on Waters Symmetry shield C8 column. A mobile phase composed of acetonitrile and buffer (18:82% (v/v). Wavelength 280 nm was used for detection. The ICH guidelines was applied for study of different parameters. The proposed method is easily and successfully applied to analyze ofloxacin and ornidazole from pharmaceutical combined formulations.

References

  1. British Pharmacopoeia (2003)., Licensing division HMSO., Norwich, 357.
  2. United States Pharmacopoeia (2004)., United States Pharmacopoeial Convention, Inc., Rockville, 1335.
  3. European Pharmacopoeia (2005)., EDQM, Council of Europe, Strasbourg, France., 5th Ed, 2131.
  4. Bhusari K.P. and Chaple D.R. (2009)., Simultaneous Spectrophotometric Estimation of ofloxacin and ornidazole in Tablet dosage Form., Asian J. Research Chem, 2(1), 60-62.
  5. Kasture V.S., Bhagat A.D., Puro N.C., More P.S. and Bhandari N.K. (2004), Spectrophotometric method for simultaneous estimation of ofloxacin and ornidazole in tablet dosage form., Indian Drugs, 41, 51-53.
  6. Ganhimathi M., Ravi T.K. and Shukla N. (2006)., Validated high performance thin layer chromatography method for simultaneous estimation of ofloxacin and ornidazole., Indian J. Pharm. Sci., 68, 838-840.
  7. Ghosh Soumya Jyoti, Darbar Soumendra, Chowdhury Partha Pratim, Chattopadhyay Shyama Prasad, Chakraborty Matish Ranjan (2010)., Simultaneous Determination and Validation of Ofloxacin and Ornidazole in Combined Dosage Pharmaceutical Formulation., International Journal of Pharm Tech Research, 2(1), 367-374.
  8. Nalini C.N., Ramachandran S., Kavitha K. and Harikrishna (2011)., Simultaneous Determination of of loxacin and ornidazole in Tablets by spectrophotometry and reverse phase HPLC., Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(3), 693-708.
  9. Miniyar Pankaj B., Mulani Afroj, Dhange Anup A, Gawande Vandana T and Kashid Arun M (2012)., Development and Validation of RP-HPLC Method for Simultaneous Determination of Ofloxacin and Ornidazole In Infusion., Am. J. PharmTech Res, , 2(5), 382-390.
  10. Dhandapani B., Thirumoorthy N., Shaik Harun Rasheed, kotaiah M.Rama and Anjaneyalu N. (2010)., Method development and validation for the simultaneous estimation of ofloxacin and ornidazole in tablets dosage form by RP-HPLC., International Journal of Pharma Sciences and Research, (1), 78-83.
  11. Puranik Manisha, Bhawsar D.V., Rathi Prachi and Yeole P.G. (2010)., Simultaneous Determination of Ofloxacin and ornidazole in Solid Dosage Form by RP-HPLC and HPTLC Techniques., Indian J. Pharm. Sci., 72(4), 513-517.
  12. Budavari S. Eds. In. (2001)., The Merck Index., Merck & co., Inc. Whitehouse Station, NJ. 13th Ed, 1213-1229.
  13. Mehta Saumil, Singh Sukhdev, Chikhalia Kishor, Shah Vishal and Saraswat Girish (2012)., Simultaneous estimation of Ofloxacin and ornidazole in combined dosage forms by dual wavelength and ratio Spectra Derivative Methods using UV-Spectrophotometer., International Journal of PharmTech Research, 4(3), 975-985.