Fourier Transform Infrared Spectroscopy (FTIR) Spectral Analysis of BSA Nanoparticles (BSA NPs) and Egg Albumin Nanoparticles (EA NPs)
- 1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, Sec-125, Noida,a-201303,UP. India
Res.J.chem.sci., Volume 6, Issue (2), Pages 29-36, February,18 (2016)
Advance protein based nanobiotechnology are now, known for its advanced approach to synthesize various nanomaterials by using various newly developed chemical and green technologies to carry out their applications in various fields of life-sciences. Now these days, Bovine serum albumin nanoparticles (BSA NPs) are very popular as non-toxic and nonviral vehicle systems for drug delivery used in number of therapeutic strategies to be considered in combating cancers, tumors, hormone associated and neurodegenerative disease. The albumin nanoparticles e.g. bovine serum albumin nanoparticles (BSA NPs) and egg albumin nanoparticles (EA NPs) are easily prepared by number of established emulsified and desolvation process to get desired particle size at nanosacle e.g. range between 100 to 300 nm with minimal size distribution. The size controlled BSA and egg albumin nanoparticles can be used as the standard cost effective, non-toxic, non-allergic and non-viral biocompatible nanodevice and nanovehicle carrier for loading of desired drug/ biological active ingredients/ hormones molecules/ chimeric-DNA with efficient target delivery system. As well as, fabrication of BSA NPs and EA NPs by using emulsified and desolvation method are done via glutaraldehyde coupling for loading of Cicer arietinum amylase that found to be better eco-friendly and cost-effective choice as compared to synthesis of other metal based nanoparticles. Sometimes, the metalonanoparticles or nanomaterials are found to be very costly and toxic because of their slow natural degradation in host and their chemically driven synthesis. Hence, the synthesis of bio-compatible BSA NPs and EA NPs might be used for their further consideration for their safe and non-toxic therapeutic applications in fields of biosensor technology, biomedical approaches and pharmaceutical industry for the treatment of various diseases. Hence, this designed Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to determine the purity of the prepared bovine serum albumin nanoparticles (BSA NPs) and egg albumin nanoparticles (EA NPs) and their active functional groups and chemical interactions for loading desired biological components or drugs that can might be further considered to increase their safe and cost effective therapeutic viability.
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