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Equilibrium study and Stability constants of mixed Ligand complexes of Bio-molecules and Amino acids with Metal ions by Potentiometric method

Author Affiliations

  • 1Department of Chemistry, University of Allahabad, Allahabad-211002, U.P., INDIA

Res.J.chem.sci., Volume 5, Issue (3), Pages 42-48, March,18 (2015)


Research of metal complexes with biologically active ligand is one of high scientific and practical interest. Formation of binary and ternary complexes of metal ions such as Cu(II), Co(II), Pb(II), Zn(II) and Cd(II) with biologically important ligand were investigated. Where nucleic acid Adenine (A) used as primary ligand and amino acid Histidine (B) used as secondary ligand. Potentiometric technique was applied for determination of complexation behavior of binary and ternary species in aqueous media at 371 C under ionic strength I = 0.1 M NaNO. Stability constant have been determined through the method suggested by Irving & Rossetti and further refined through SCOGS computer program. The mixed ligand ternary complexes were occurring simultaneously in most cases generally at high pH with gradually declination of binary complexes of particular metal complexes. The percentage of ternary species of 56 at 6.4 pH for A-Cu(II)-B, 67.7 at 6.3 pH for A-Co(II)-A, 65.56 at 7.2 pH for A-Pb(II)-B, 48.5 at 7.4 pH for A-Zn(II)-B and 56.5 at 7.3 pH for A-Cd(II)-B. The overall stability constant of mixed ligand Adenine(A)-M(II)-Histidine(B) ternary system are obtained in the following order A-Cu(II)-B > ACo(II)-B>A -Pb(II)-B > A-Zn(II)-B> A-Cd(II)-B.


  1. Mishra G. K, Krishna V. and Prakash R., Synthesis and characterization of novel hetrobinuclear mercury (II)-DTPA M(II) complexes: Electro catalytic and sensor applications, Synt. React. Inorganic, Met.-Org. Nano-Met. Chem.,39,124-128, (2009)
  2. Singh D., Gautam R. K., Kumar R., Shukla B K., Shankar V. and Krishna V., Citric acid coated magnetic nanoparticles: Synthesis, characterization and application in removal of Cd(II) ions from aqueous solution, J. Wat. Pro. Eng., 4, 233-241,(2014)
  3. Singh D., Chandra H and Krishna V., Valine-Coated Magnetic Nanoparticles Synthesis, Characterization, and Application in Removal of Cd(II) Ions from Aqueous Solution, Sep. Sci. Technol., 50 1-9 (2015)
  4. Singh D., Chandra H. and Krishna V., Potentiometric study on mixed ligand complexes of some biometals with aspartic acid as primary ligand and thiouracil as secondary ligand, Int. Journal of Inorg. Bio. Inorg. Chem.,3(4), 65-69 (2013)
  5. Singh D., Chandra H., Shankar V. and Krishna V., Mixed ligand ternary complexes of some transition metals with aspartic acid as primary ligand and cytosine as secondary ligand: Equilibrium study, Chemical speciation and Stability constant, Chem. Environ., 4(1), 48-53 (2014)
  6. Bartaria D., Chandra P and Krishna V., A Study on Speciation and Coordination Tendency of Glutamic Acid and Uracil for Ternary Complexation towards some toxic metal ions, Chem. Sci. Rev. Lett., 1(4), 201-208 (2013)
  7. Altun Y and Koseoglu F., Stability of Copper(II), Nickel(II) and Zinc(II) Binary and ternary complexes of histidine, histamine and glycine in aqueous solution, J. Solution Chem, 34 (2), 213-231 (2005)
  8. Deschamps P., Kulkarni P.P., Gautam-Basak M., Sarkar B., The Saga of copper(II)-L-histidine, 249, 895-909 (2005)
  9. Watabe M., Kai M., Goto K., Ohmuro H., Furukawa S., Chikaraishi N., Takayama T. and Koike Y., Preparation of platinum (II) complexes with l-serine using KI. X-ray crystal structure, HPLC and 195 Pt NMR spectra, J.Inorg.Biochem., 97(2), 240- (2003)
  10. Parisi A.F. and Vallee B.L., Zinc metalloenzymes: Characteristics and significance in biology and medicine, Am J Clin Nutr., 22 12221239 (1969)
  11. Ammar R.A., Al-Mutiri E. and Abdalla M.A., Equilibrium study of the mixed complexes of copper(II) with adenine and amino acids in aqueous solution, J. Solution Chem., 39 727-737 (2010)
  12. Theo P.A. Kruck and Sarkar B., Equilibria of the Simultaneous Existing Multiple Species In the Copper(II)-L-Histidine System, Can. J.Chem., 51 3549 (1972)
  13. Kumar G., Synthesis and characterization of mixed ligand copper(ii) complexes of histidine and hippuric acid, chemistry.,20(1), 1-8 (2011)
  14. Mumthaz A.K. and Parameswaran P., Physico-chemical studies and thermal decomposition kinetics of some metal complexes of Dibenzoylmethane L-histidine, Asian J. Chem.,22(1) 61-65 (2010)
  15. Sinha S., Shukla P, Singh P. P and Krishna V., Chemical distribution and structure of quaternary metal chelates in aqueous solution involving asparagine and uracil, Chem. Sci. Trans.,3(2), 576-581 (2014)
  16. Carrie A. M., Walker M. D and Williams D. R, J. Chem. Soc. Dalton Trans., 1012-1015 (1976)
  17. Ramteke A and Narwadeb M., Studies of stability constants of the complexes of chlorosubstituted pyrazoles and pyrazoline with Cu(II),Ni(II),Co(II) and Nd(III) metal ions in 70% dioxane-water mixture at 0.1 M ionic strength, Arch. Appl. Sci. Res., 5(1) 231-237 (2013)
  18. Bartaria D., Shukla V.P., and Krishna V., Chemical speciation and thermodynamic stability of quaternary mixed chelates of bio-metals involving lysine, proline and uracil, J. Indian Chem. Soc.,86, 9-13 (2009)
  19. Tripathi N.K. and Krishna V., Speciation of nickel (II) and cobalt (II) quaternary complexes of L-Aspartic acid and Uracil. Ultra Chem., 9(1) 83-86 (2013)
  20. Kayande Abdulbaset D.D., Zaid A., Pradhan V. and Farooqui M., Potentiometric study on stability of binary and ternary complexes of nicotinamide in aqueous solution with copper (ii) metal ion, International, J. Sci. Nat., 3(2) 416-419 (2012)
  21. Kumar K. B., Naik B., Kumar A., Raju S., and Rao G.N., Speciation studies of l-histidine complexes of Pb(II), Cd(II), and Hg(II) in dmso- water mixtures, Int. J. Inorg. Chem.,2012 1-9 (2012)
  22. Shukla V.P, Sinha S and Krishna K., Multiple equilibria and chemical distribution of some bio metals with amide -aminosuccinate and- aminoisoverate as primary ligand and 5-methyl 2, 4 dioxopyrimidine as secondary ligand, J. Appl. Chem.,4(6) 21-26 (2013)
  23. Kumar K., Dwivedi D. K., Equilibrium Based Computer Studies of Hetrobinuclear Complexes of Toxic Metal Ions Involving Biologically Significant Ligands, Int. J. Inno. Res. Sci., Eng. Tech.,3(7)(2014)